Biorepository and Tissue Technology Shared Resource (BTTSR) The BTTSR provides for the acquisition, multi-modality processing, and archiving of well-annotated human biospecimens that is seamlessly integrated with histology and tissue analysis services. Three major activities are provided by this shared resource: 1) the biorepository for human tumor specimens that are acquired during diagnostic and therapeutic procedures from consented subjects with associated blood, urine (when applicable), and CSF (when applicable), and that are linkable to clinical and diagnostic and made available to MCC investigators;2) tissue histology and technology for state-of-the-art molecular morphology/biomarker applications, and 3) histopathologic expertise for validation of diagnostic parameters on biorepository specimens, and assistance in the interpretation of human and mouse tissue pathology. Each major activity is coordinated by an expert faculty co-director. Dr. Vandenberg (STT), an experienced pathologist, is overall Director of the BTTSR, Dr. Schwab (STT) oversees the biorepository clinical affairs and sample collection and distribution, and Dr. Varki (STT) oversees the histology and tissue technology applications in human samples and animal models. The BTTSR assists in the formulation of targeted prospective collections for clinical trials or for translational research projects directed at biospecimens that need specific parameters, in addition to routine tissue collection during diagnostic or therapeutic procedures. Tissue technology includes multi-label immunohistochemistry, multiplexing immunofluorescence, and in situ hybridization in addition to analytical microscopy for molecular morphology, including spectral imaging. The state-of-the-art laser-capture microscopy in the tissue technology resource provides an important resource for performing genomic/expression profiling and proteomics on tissue sections with complex tumor cell heterogeneity and complements the biomarker discovery capabilities performed in this facility. During 2012, 40 MCC investigators representing five programs used Histology services and 11 investigators used the Biorepository accounting for 97% of utilization, and the combined resources received 10% of their funding from the CCSG. This is a new shared resource created from a merger of the previous Histology and Biorepository shared resources.

Public Health Relevance

The availability of the Biorepository and Tissue Technology Shared Resource enables our researchers to access annotated, optimally preserved human biospecimens for translational research. This Shared Resource also facilitates both routine histologic analysis and multi-modality detection of biomarkers in complex tumor tissue and animal models, and assists in the histopathologic interpretation of these biomarkers.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023100-28
Application #
8934940
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-07-21
Project End
2019-04-30
Budget Start
2014-07-21
Budget End
2015-04-30
Support Year
28
Fiscal Year
2014
Total Cost
$367,129
Indirect Cost
$127,914
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Akers, Johnny C; Ramakrishnan, Valya; Yang, Isaac et al. (2016) Optimizing preservation of extracellular vesicular miRNAs derived from clinical cerebrospinal fluid. Cancer Biomark 17:125-32
Hoffmann, Hanne M; Trang, Crystal; Gong, Ping et al. (2016) Deletion of Vax1 from Gonadotropin-Releasing Hormone (GnRH) Neurons Abolishes GnRH Expression and Leads to Hypogonadism and Infertility. J Neurosci 36:3506-18
Baumgartner, Joel M; Kwong, Thomas G; Ma, Grace L et al. (2016) A Novel Tool for Predicting Major Complications After Cytoreductive Surgery with Hyperthermic Intraperitoneal Chemotherapy. Ann Surg Oncol 23:1609-17
Meyskens Jr, Frank L; Mukhtar, Hasan; Rock, Cheryl L et al. (2016) Cancer Prevention: Obstacles, Challenges and the Road Ahead. J Natl Cancer Inst 108:
Hussain, Timon; Savariar, Elamprakash N; Diaz-Perez, Julio A et al. (2016) Surgical molecular navigation with ratiometric activatable cell penetrating peptide for intraoperative identification and resection of small salivary gland cancers. Head Neck 38:715-23
Singh, Alok R; Joshi, Shweta; Zulcic, Muamera et al. (2016) PI-3K Inhibitors Preferentially Target CD15+ Cancer Stem Cell Population in SHH Driven Medulloblastoma. PLoS One 11:e0150836
Liu, Lin; Messer, Karen; Baron, John A et al. (2016) A prognostic model for advanced colorectal neoplasia recurrence. Cancer Causes Control 27:1175-85
Nuyen, Brian A; Fox, Rina S; Malcarne, Vanessa L et al. (2016) Excessive Daytime Sleepiness as an Indicator of Depression in Hispanic Americans. Hisp Health Care Int 14:116-23
Ghosh, Pradipta; Aznar, Nicolas; Swanson, Lee et al. (2016) Biochemical, Biophysical and Cellular Techniques to Study the Guanine Nucleotide Exchange Factor, GIV/Girdin. Curr Protoc Chem Biol 8:265-298
Johns, Claire; Seav, Susan M; Dominick, Sally A et al. (2016) Informing hot flash treatment decisions for breast cancer survivors: a systematic review of randomized trials comparing active interventions. Breast Cancer Res Treat 156:415-26

Showing the most recent 10 out of 699 publications