Biorepository and Tissue Technology Shared Resource (BTTSR) The BTTSR provides for the acquisition, multi-modality processing, and archiving of well-annotated human biospecimens that is seamlessly integrated with histology and tissue analysis services. Three major activities are provided by this shared resource: 1) the biorepository for human tumor specimens that are acquired during diagnostic and therapeutic procedures from consented subjects with associated blood, urine (when applicable), and CSF (when applicable), and that are linkable to clinical and diagnostic and made available to MCC investigators;2) tissue histology and technology for state-of-the-art molecular morphology/biomarker applications, and 3) histopathologic expertise for validation of diagnostic parameters on biorepository specimens, and assistance in the interpretation of human and mouse tissue pathology. Each major activity is coordinated by an expert faculty co-director. Dr. Vandenberg (STT), an experienced pathologist, is overall Director of the BTTSR, Dr. Schwab (STT) oversees the biorepository clinical affairs and sample collection and distribution, and Dr. Varki (STT) oversees the histology and tissue technology applications in human samples and animal models. The BTTSR assists in the formulation of targeted prospective collections for clinical trials or for translational research projects directed at biospecimens that need specific parameters, in addition to routine tissue collection during diagnostic or therapeutic procedures. Tissue technology includes multi-label immunohistochemistry, multiplexing immunofluorescence, and in situ hybridization in addition to analytical microscopy for molecular morphology, including spectral imaging. The state-of-the-art laser-capture microscopy in the tissue technology resource provides an important resource for performing genomic/expression profiling and proteomics on tissue sections with complex tumor cell heterogeneity and complements the biomarker discovery capabilities performed in this facility. During 2012, 40 MCC investigators representing five programs used Histology services and 11 investigators used the Biorepository accounting for 97% of utilization, and the combined resources received 10% of their funding from the CCSG. This is a new shared resource created from a merger of the previous Histology and Biorepository shared resources.
The availability of the Biorepository and Tissue Technology Shared Resource enables our researchers to access annotated, optimally preserved human biospecimens for translational research. This Shared Resource also facilitates both routine histologic analysis and multi-modality detection of biomarkers in complex tumor tissue and animal models, and assists in the histopathologic interpretation of these biomarkers.
|Singh, Siddharth; Loomba, Rohit (2018) Role of two-dimensional shear wave elastography in the assessment of chronic liver diseases. Hepatology 67:13-15|
|Hartman, Sheri J; Nelson, Sandahl H; Myers, Emily et al. (2018) Randomized controlled trial of increasing physical activity on objectively measured and self-reported cognitive functioning among breast cancer survivors: The memory & motion study. Cancer 124:192-202|
|Hoffmann, Hanne M; Gong, Ping; Tamrazian, Anika et al. (2018) Transcriptional interaction between cFOS and the homeodomain-binding transcription factor VAX1 on the GnRH promoter controls Gnrh1 expression levels in a GnRH neuron maturation specific manner. Mol Cell Endocrinol 461:143-154|
|Liu, Xuxiang; Cao, Minghui; Palomares, Melanie et al. (2018) Metastatic breast cancer cells overexpress and secrete miR-218 to regulate type I collagen deposition by osteoblasts. Breast Cancer Res 20:127|
|Huang, Justin K; Carlin, Daniel E; Yu, Michael Ku et al. (2018) Systematic Evaluation of Molecular Networks for Discovery of Disease Genes. Cell Syst 6:484-495.e5|
|Kalyanaraman, Hema; Schwaerzer, Gerburg; Ramdani, Ghania et al. (2018) Protein Kinase G Activation Reverses Oxidative Stress and Restores Osteoblast Function and Bone Formation in Male Mice With Type 1 Diabetes. Diabetes 67:607-623|
|Hartman, Sheri J; Marinac, Catherine R; Cadmus-Bertram, Lisa et al. (2018) Sedentary Behaviors and Biomarkers Among Breast Cancer Survivors. J Phys Act Health 15:1-6|
|Wu, Yan; Tamayo, Pablo; Zhang, Kun (2018) Visualizing and Interpreting Single-Cell Gene Expression Datasets with Similarity Weighted Nonnegative Embedding. Cell Syst 7:656-666.e4|
|Dow, Michelle; Pyke, Rachel M; Tsui, Brian Y et al. (2018) Integrative genomic analysis of mouse and human hepatocellular carcinoma. Proc Natl Acad Sci U S A 115:E9879-E9888|
|Que, Xuchu; Hung, Ming-Yow; Yeang, Calvin et al. (2018) Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. Nature 558:301-306|
Showing the most recent 10 out of 862 publications