The Norris Cotton Cancer Center (NCCC) Molecular Biology Shared Resource Laboratory (MBSR) has been operating since 1995 as a core facility. This core initially was developed within the Cancer Center, but was quickly expanded to provide services to other laboratories with the addition of other sources of support. It has operated as a university cost center since 1997, but remains a central and important shared resource for NCCC investigators, who represent a major proportion of its user base. In 2000, proteomics services were added to this shared resource. In 2007 these were split off as the new Proteomics Shared Resource (PRSR) described separately elsewhere. The MBSR provides state-of-the-art molecular biology support including DMA and RNA oligonucleotide synthesis, DMA sequencing, fragment analysis, traditional and real-time PCR services, and various imaging support services for the NCCC membership. The MBSR also collaborates with the Proteomics, Bioinformatics, Genomics, and Biostatistics Shared Resources in providing seamless support for the investigator who is applying these tools for genomic level analyses as part of the Integrative Biology Group (IBG). The MBSR currently provides 54 NCCC members and their laboratories with services which totaled $131 K in chargebacks for FY 2007 ($273K total chargebacks). The NCCC users represented 47% of the total laboratories using this facility at Dartmouth (116 labs total), and their usage constituted 48% of the total usage based on chargeback fees and 44% based on units of use. The total chargebacks for this core were $273 K for FY 2007, and the total operating budget was $597K, requiring $324K in subvention which was derived from the NCCC Core Grant ($57K) and other institutional resources ($267K). This shared resource is requesting a budget of $52,787 from the NCCC Core Grant for the first year of this renewal, representing a level comparable to the current year's support and representing approximately 14% of its total operating budget for FY 2009 of approximately $378K (note that the decrease in subvention support and total budget is due to the splitting of MBSR and PRSR and concomitant reduction in services, costs, and staffing for MBSR). The MBSR continues to add and upgrade instrumentation, including recent upgrades to its DNA sequencing services. It plans to add the new Solexa technology from Agilent in the near future, which will facilitate important laboratory research and allow the NCCC investigators to comprehensively analyze the genetic polymorphisms for the entire genomes of individual cancer patients and thereby provide a means of customizing individual diagnosis and treatment. The overall goal of the MBSR is to continue to provide NCCC researchers with state-of-the-art technical support and services for their cancer research at as low a rate as possible so that they can continue to successfully meet their research objectives individually and collectively.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Dartmouth College
United States
Zip Code
Zhang, Sai; Zhou, Jingtian; Hu, Hailin et al. (2016) A deep learning framework for modeling structural features of RNA-binding protein targets. Nucleic Acids Res 44:e32
Macura, Sherrill L; Lathrop, Melissa J; Gui, Jiang et al. (2016) Blocking CXCL9 Decreases HIV-1 Replication and Enhances the Activity of Prophylactic Antiretrovirals in Human Cervical Tissues. J Acquir Immune Defic Syndr 71:474-82
Whipple, Chery A; Boni, Andrea; Fisher, Jan L et al. (2016) The mitogen-activated protein kinase pathway plays a critical role in regulating immunological properties of BRAF mutant cutaneous melanoma cells. Melanoma Res 26:223-35
Sargent, Jennifer L; Li, Zhenghui; Aliprantis, Antonios O et al. (2016) Identification of Optimal Mouse Models of Systemic Sclerosis by Interspecies Comparative Genomics. Arthritis Rheumatol 68:2003-15
Hill, Courtney A; Beach, Michael; Smith, Mark C et al. (2016) Incidence of and Factors Associated With Hypogeusia in Healthy Children. JAMA Otolaryngol Head Neck Surg 142:229-33
Kim, Jung-Sik; He, Xiaoyuan; Orr, Bernardo et al. (2016) Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet 12:e1005865
Yang, Wei; Hosford, Sarah R; Dillon, Lloye M et al. (2016) Strategically Timing Inhibition of Phosphatidylinositol 3-Kinase to Maximize Therapeutic Index in Estrogen Receptor Alpha-Positive, PIK3CA-Mutant Breast Cancer. Clin Cancer Res 22:2250-60
Beach, Michael L; Cohen, Daniel M; Gallagher, Susan M et al. (2016) Major Adverse Events and Relationship to Nil per Os Status in Pediatric Sedation/Anesthesia Outside the Operating Room: A Report of the Pediatric Sedation Research Consortium. Anesthesiology 124:80-8
Allaway, Robert J; Fischer, Dawn A; de Abreu, Francine B et al. (2016) Genomic characterization of patient-derived xenograft models established from fine needle aspirate biopsies of a primary pancreatic ductal adenocarcinoma and from patient-matched metastatic sites. Oncotarget 7:17087-102
Liu, Yu-Chen; Li, Jian-Rong; Sun, Chuan-Hu et al. (2016) CircNet: a database of circular RNAs derived from transcriptome sequencing data. Nucleic Acids Res 44:D209-15

Showing the most recent 10 out of 1435 publications