The Transgenics and Genetic Constructs Shared Resource (TGCSR) during much of the current funding period provided three main services: (1) generation of transgenic mice by DNA injection in outbred, mixed inbred and inbred mouse strains;(2) culture and transfection of ES cells to generate "knockout" ES clones; and (3) production of "knockout" mice using genetically modified ES cells. On average, the TGCSR generated 17 novel mouse models per year, consisting of approximately 11 transgenic mouse lines from individual DNA constructs and 6 ES cell-derived mutant mouse lines. The TGCSR provided various other services, including rederivation of pathogen-infected transgenic mouse lines, cryopreservation of transgenic mouse embryos and sperm, assistance with husbandry and genotyping of transgenic mouse lines, and assistance with special surgeries or IV injections for experimental purposes. Recently, the TGCSR has been reorganized to maximize efficiency and expand services to Dartmouth investigators. Transgenic and Genetic Construct Shared Resource (TGCSR) provides the additional major services of genetic constructs using "recombineering" in E. coli and yeast, purifying DNA for genotyping mice or ES cells, performing PCR or Southern blotting to genotype mice or ES cells, and karyotyping ES clones. The TGCSR services start with planning a new mouse model and end with genetically modified animals, with no further labor from the investigator. These expanded, start-to-finish services will support the generation of new experimental models by Dartmouth investigators who are not molecular biologists in their labs. In order to more efficiently utilize resources and respond to current directives from the NCI requesting that cancer centers seek regional collaboration and combine utilization of shared resources whenever feasible, Morris Cotton Cancer Center has entered into an agreement with the Jackson Laboratory (JAX) to have the mouse embryo manipulation for the TGCSR done by the transgenic shared resource at JAX. All previously provided services that do not involve manipulation of mouse embryos will continue to be performed at NCCC by the TGCSR. Under this new arrangement, the TGCSR director will be the conduit for all communications between Dartmouth investigators and JAX, for providing reagents to JAX, billing investigators, and financially compensating JAX. The vast experience and resources of JAX in the area of mouse genetics will provide even better service for NCCC investigators, and, since JAX makes a direct delivery of pathogen-free mice to Dartmouth every week, the movement of transgenic mice will be efficient and convenient for all. By partnering with JAX, the world's foremost facility for mouse genetics, and by expanding our services to include genetic construct production, karyotyping, and genotyping, we will better serve NCCC investigators.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023108-35
Application #
8463396
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
2014-11-30
Budget Start
2012-12-01
Budget End
2013-11-30
Support Year
35
Fiscal Year
2013
Total Cost
$81,832
Indirect Cost
$30,039
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
Hou, Huagang; Krishnamurthy Nemani, Venkata; Du, Gaixin et al. (2015) Monitoring oxygen levels in orthotopic human glioma xenograft following carbogen inhalation and chemotherapy by implantable resonator-based oximetry. Int J Cancer 136:1688-96
Gilbert-Diamond, Diane; Li, Zhigang; Adachi-Mejia, Anna M et al. (2014) Association of a television in the bedroom with increased adiposity gain in a nationally representative sample of children and adolescents. JAMA Pediatr 168:427-34
Sheen, Mee Rie; Lizotte, Patrick H; Toraya-Brown, Seiko et al. (2014) Stimulating antitumor immunity with nanoparticles. Wiley Interdiscip Rev Nanomed Nanobiotechnol 6:496-505
Koestler, Devin C; Li, Jing; Baron, John A et al. (2014) Distinct patterns of DNA methylation in conventional adenomas involving the right and left colon. Mod Pathol 27:145-55
Soderquist, Ryan; Pletnev, Alexandre A; Danilov, Alexey V et al. (2014) The putative BH3 mimetic S1 sensitizes leukemia to ABT-737 by increasing reactive oxygen species, inducing endoplasmic reticulum stress, and upregulating the BH3-only protein NOXA. Apoptosis 19:201-9
Tang, Hongwei; Wei, Peng; Duell, Eric J et al. (2014) Axonal guidance signaling pathway interacting with smoking in modifying the risk of pancreatic cancer: a gene- and pathway-based interaction analysis of GWAS data. Carcinogenesis 35:1039-45
Toraya-Brown, Seiko; Sheen, Mee Rie; Zhang, Peisheng et al. (2014) Local hyperthermia treatment of tumors induces CD8(+) T cell-mediated resistance against distal and secondary tumors. Nanomedicine 10:1273-85
O'Connor, Megan A; Green, William R (2014) Use of IRF-3 and/or IRF-7 knockout mice to study viral pathogenesis: lessons from a murine retrovirus-induced AIDS model. J Virol 88:2349-53
Tichauer, Kenneth M; Deharvengt, Sophie J; Samkoe, Kimberley S et al. (2014) Tumor endothelial marker imaging in melanomas using dual-tracer fluorescence molecular imaging. Mol Imaging Biol 16:372-82
Busch, Alexander M; Galimberti, Fabrizio; Nehls, Kristen E et al. (2014) All-trans-retinoic acid antagonizes the Hedgehog pathway by inducing patched. Cancer Biol Ther 15:463-72

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