Immune Monitoring & Flow Cytometry (IMFC) Immune Monitoring and Flow Cytometry were separate CCSG-funded shared resources that were combined in July 2010 into a single shared resource, the Immune Monitoring & Flow Cytometry (IMFC) Shared Resource. For ease of reference, the unit publicizes its services to clients as DartLab. Flow Cytometry is located at both the Lebanon and Hanover sites, and Immune Monitoring is located within Norris Cotton Cancer Center's (NCCC) Lebanon facilities. Equipment offered by Flow Cytometry includes a single FACSAria I cell sorter operated by a dedicated flow cytometrist at the Lebanon site, in addition to a 10-color Gallios, 7-color MACSQuant, 4-color FACSCalibur and 3-color FACScan. There is an 8-color MACSQuant VYB and a 6-color FACSCanto at the Hanover site. Equipment offered by Immune Monitoring includes a Zeiss enzyme-linked immunoSpot assay (ELISPOT) reader, two multiplex cytokine analyzers: a Bio-Plex Array Reader and a Sector Imager (MSD); two automated magnetic bead cell separators: an autoMACS and a Robosep; and an 8-color MACSQuant 10. Services, used by both clinicians and basic scientists, include peripheral blood mononuclear cell isolation and cryopreservation, plasma/serum isolation and cryopreservation, multiplex cytokine analysis, lymphoproliferation, ELISPOT, up to 10-color phenotyping, including multimers and intracellular staining; and customized immunoassays. Flow Cytometry has allowed multiple NCCC PIs to sort cell subsets, including Ernst (CM), Dmitrovsky (CM/MT), Noelle (ICI), and Wang (ICI). The use of multicolor flow cytometry has allowed Stan (CM), Dmitrovsky (CM/MT), Turk (ICI), Ernstoff (ICI), and Mullins (ICI) to subset tumor-infiltrating cells, especially regulatory T cells. Multiplex cytokine analysis has been used by multiple NCCC PIs, including Karagas (CE), Ernstoff (ICI), Sentman (ICI), Turk (ICI), Mullins (ICI), and Stan (CM). Immune Monitoring has customized assays for Lansigan (MT), Kinlaw (MT), and Noelle (ICI). The IMFC is directed by Dr Jacqueline Channon Smith, who holds a Ph.D. in innate immunity, overseeing two experienced laboratory managers, one of whom is the dedicated cell sorter cytometrist; a research associate who holds a Ph.D. in immunology, and specialists who are cross-trained in flow cytometry and immunoassay techniques. The IMFC is used by a total of 65 principal investigators across all six NCCC programs: Cancer Control (1), Cancer Epidemiology (3), Cancer Mechanisms (17), Molecular Therapeutics (20), Cancer Imaging and Radiobiology (5), and Immunology and Cancer Immunotherapy (19).

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023108-40
Application #
9616818
Study Section
Subcommittee I - Career Development (NCI)
Project Start
Project End
Budget Start
2018-12-01
Budget End
2019-11-30
Support Year
40
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
Shee, Kevin; Jiang, Amanda; Varn, Frederick S et al. (2018) Cytokine sensitivity screening highlights BMP4 pathway signaling as a therapeutic opportunity in ER+ breast cancer. FASEB J :fj201801241R
Rodriguez-Garcia, Marta; Fortier, Jared M; Barr, Fiona D et al. (2018) Aging impacts CD103+ CD8+ T cell presence and induction by dendritic cells in the genital tract. Aging Cell 17:e12733
Shajani-Yi, Zahra; de Abreu, Francine B; Peterson, Jason D et al. (2018) Frequency of Somatic TP53 Mutations in Combination with Known Pathogenic Mutations in Colon Adenocarcinoma, Non-Small Cell Lung Carcinoma, and Gliomas as Identified by Next-Generation Sequencing. Neoplasia 20:256-262
Szczepiorkowski, Zbigniew M; Burnett, Christine A; Dumont, Larry J et al. (2018) Apheresis buffy coat collection without photoactivation has no effect on apoptosis, cell proliferation, and total viability of mononuclear cells collected using photopheresis systems. Transfusion 58:943-950
Bossé, Yohan; Amos, Christopher I (2018) A Decade of GWAS Results in Lung Cancer. Cancer Epidemiol Biomarkers Prev 27:363-379
Pande, Mala; Joon, Aron; Brewster, Abenaa M et al. (2018) Genetic susceptibility markers for a breast-colorectal cancer phenotype: Exploratory results from genome-wide association studies. PLoS One 13:e0196245
Smith, T Jarrod; Sondermann, Holger; O'Toole, George A (2018) Co-opting the Lap System of Pseudomonas fluorescens To Reversibly Customize Bacterial Cell Surfaces. ACS Synth Biol 7:2612-2617
Gorlova, Olga Y; Li, Yafang; Gorlov, Ivan et al. (2018) Gene-level association analysis of systemic sclerosis: A comparison of African-Americans and White populations. PLoS One 13:e0189498
Schmit, Stephanie L; Edlund, Christopher K; Schumacher, Fredrick R et al. (2018) Novel Common Genetic Susceptibility Loci for Colorectal Cancer. J Natl Cancer Inst :
Cai, Yunliang; Wu, Shaoju; Zhao, Wei et al. (2018) Concussion classification via deep learning using whole-brain white matter fiber strains. PLoS One 13:e0197992

Showing the most recent 10 out of 1911 publications