Biology Shared Resource Group The DNA Sequencing Shared Resource (SEQ-SR) is part of the campus-wide Purdue University Genomics Facility that provides equal access to all Purdue University Center for Cancer Research (PCCR) investigators. Established in 1993, the SEQ-SR has provided PCCR members for the past 20 years with cost effective, quality services. The services provided by the DNA Sequencing Shared Resource (SEQ-SR) supported 40 Purdue Center for Cancer Research (PCCR) investigators' research projects from all four of PCCR's Research Programs in this current year. In addition to the standard DNA sample Sanger sequencing, the SEQ-SR is developing and refining next-generation services that are critical to the research needs of PCCR members' future research plans. Members rely on the SEQ-SR to sequence genes and various gene constructs, and verify sequences containing `installed' mutations, among other uses. The low-throughput laboratory service offered by the SEQ-SR facility additionally supports the Transgenic Mouse Core Facility Shared Resource (TMCF-SR). Specifically, the SEQ-SR helps ensure that the targeting and other DNA containing constructs that will be injected into rodents, are correct. Given the fairly low dollar cost of obtaining sequence data of a few samples, it is important to assess all the costs involved to researchers in utilizing a service of this type by convenience, rapid turn-around times, persistent data storage and availability of staff for consultation with the investigator.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023168-37
Application #
9369059
Study Section
Special Emphasis Panel (ZCA1)
Program Officer
Ptak, Krzysztof
Project Start
Project End
Budget Start
2017-07-01
Budget End
2018-06-30
Support Year
37
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Purdue University
Department
Type
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Alpsoy, Aktan; Dykhuizen, Emily C (2018) Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes. J Biol Chem 293:3892-3903
Larocque, Elizabeth A; Naganna, N; Opoku-Temeng, Clement et al. (2018) Alkynylnicotinamide-Based Compounds as ABL1 Inhibitors with Potent Activities against Drug-Resistant CML Harboring ABL1(T315I) Mutant Kinase. ChemMedChem 13:1172-1180
Kumari, Rashmi; Silic, Martin R; Jones-Hall, Yava L et al. (2018) Identification of RECK as an evolutionarily conserved tumor suppressor gene for zebrafish malignant peripheral nerve sheath tumors. Oncotarget 9:23494-23504
VerHeul, Ross; Sweet, Craig; Thompson, David H (2018) Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction. Biomater Sci 6:863-876
Poh, Scott; Chelvam, Venkatesh; Ayala-López, Wilfredo et al. (2018) Selective liposome targeting of folate receptor positive immune cells in inflammatory diseases. Nanomedicine 14:1033-1043
Coleman, Rachel A; Trader, Darci J (2018) A Sensitive High-Throughput Screening Method for Identifying Small Molecule Stimulators of the Core Particle of the Proteasome. Curr Protoc Chem Biol 10:e52
AlAbdi, Lama; He, Ming; Yang, Qianyi et al. (2018) The transcription factor Vezf1 represses the expression of the antiangiogenic factor Cited2 in endothelial cells. J Biol Chem 293:11109-11118
Lee, Hyeong-Min; Clark, Ellen P; Kuijer, M Bram et al. (2018) Characterization and structure-activity relationships of indenoisoquinoline-derived topoisomerase I inhibitors in unsilencing the dormant Ube3a gene associated with Angelman syndrome. Mol Autism 9:45
Liu, Yunhua; Xu, Hanchen; Van der Jeught, Kevin et al. (2018) Somatic mutation of the cohesin complex subunit confers therapeutic vulnerabilities in cancer. J Clin Invest 128:2951-2965
Hall, Hana; Ma, Jingqun; Shekhar, Sudhanshu et al. (2018) Blue light induces a neuroprotective gene expression program in Drosophila photoreceptors. BMC Neurosci 19:43

Showing the most recent 10 out of 436 publications