Observations of molecular changes in patient tissues or caused by genetic or experimental manipulations in mice and cancer cells in culture critically depend on histological and immunohistological analyses in cells and tissues through advanced imaging techniques. The mission of the Cell Imaging and Histopathology Shared Resource is to provide state-of-the-art imaging and histopathology tools, training, and services to Cancer Center researchers. The Shared Resource is divided into two adjacent Facilities: 1) The Cell /mag/ng facility houses four confocal microscopes and five additional fluorescence microscopes. All the microscopes are equipped with advanced digital cameras and image analysis software. Available systems include laser scanning confocal microscopes with single and multi-photon lasers, as well as a spinning disk confocal microscope for extended live cell imaging. Time-lapse imaging systems are available to study cell motility, proliferation, and differentiation, with FRET and TIRF systems to image intracellular dynamic interactions. A full range of wide-field imaging approaches are also supported. The facility transmission electron microscope service provides analysis of cell and tissue ultrastructure. Facility staff maintains the imaging equipment and provides investigator training and support for imaging and image analysis, as well as imaging service, and active development of new imaging tools. 2) The Histopathology facility offers the preparation, sectioning, staining and analysis of tissue samples for histological and histopathological analyses and probe-specific detection of cellular and molecular changes in tissue sections. For analysis of identified cells or cell groups within tissue sections, the facility offers Laser Capture Microdissection services. An automated slide scanning system affords efficient histological data acquisition, image archiving, analysis and quantitation. A staff pathologist with expertise in both human and mouse specimens provides consultation, as well as assistance with tissue microarrays. The Facility staff provides consultation, user training, development and dissemination of imaging-, histology- and immunohistochemical protocols, and assistance with instrumentation for efficient histology data documentation, analysis and storage. The Shared Resource supported the studies of 35 Cancer Center Members in the past year, and is essential to advance the understanding of molecular and cellular events leading to cancer development and spreading. Overall, $185,234 in CCSG support is requested in the first year, representing 18.4% of the total projected annual operating budget.

Public Health Relevance

Imaging and Histopathology resources are essential for understanding the molecular, cellular, and tissue components of cancers. The advanced shared microscopes provide cost-effective access to well maintained shared equipment, and the expertise of the staff, including a pathologist, provide important support for research.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA030199-32
Application #
8473822
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
32
Fiscal Year
2013
Total Cost
$218,635
Indirect Cost
$100,414
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Fujita, Yu; Khateb, Ali; Li, Yan et al. (2018) Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis. Cell Rep 24:3296-3311.e6
Scully, Kathleen M; Lahmy, Reyhaneh; Signaevskaia, Lia et al. (2018) E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53. Cell Mol Gastroenterol Hepatol 6:181-198
Borlido, Joana; Sakuma, Stephen; Raices, Marcela et al. (2018) Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol 19:594-605
Follis, Ariele Viacava; Llambi, Fabien; Kalkavan, Halime et al. (2018) Regulation of apoptosis by an intrinsically disordered region of Bcl-xL. Nat Chem Biol 14:458-465
Pathria, Gaurav; Scott, David A; Feng, Yongmei et al. (2018) Targeting the Warburg effect via LDHA inhibition engages ATF4 signaling for cancer cell survival. EMBO J 37:
Sun, Younguk; Chen, Bo-Rui; Deshpande, Aniruddha (2018) Epigenetic Regulators in the Development, Maintenance, and Therapeutic Targeting of Acute Myeloid Leukemia. Front Oncol 8:41
Ekanayake, Vindana; Nisan, Danielle; Ryzhov, Pavel et al. (2018) Lipoprotein Particle Formation by Proapoptotic tBid. Biophys J 115:533-542
Diez-Cuñado, Marta; Wei, Ke; Bushway, Paul J et al. (2018) miRNAs that Induce Human Cardiomyocyte Proliferation Converge on the Hippo Pathway. Cell Rep 23:2168-2174
Wang, Yang; Li, Yue; Yue, Minghui et al. (2018) N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci 21:195-206
Lundquist, Mark R; Goncalves, Marcus D; Loughran, Ryan M et al. (2018) Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating Autophagy. Mol Cell 70:531-544.e9

Showing the most recent 10 out of 599 publications