The Flow Cytometry Shared Resource offers Cancer Center users multi-parametric evaluation and high speed enrichment of phenotypically distinct cell populations using flow cytometry. For analytical flow cytometry, investigators have a choice of full-service analysis, or receiving training and then independent use of the two analytical flow cytometers (BD FACSort and BD FACSCanto). Investigator training and consulting is also provided on analytical software including CellQuest, FlowJo, and ModFitLT. For high-speed cell sorting, the facility provides experimental planning and full-service cell sorting by an expert operator, utilizing a BD FACSVantageSE DiVa. This instrument was recently equipped with a custom biosafety cabinet to enable sorting of cells infected with viral vectors, better protecting both the operator and the samples. The experienced Facility Director, Mr. Altman, provides investigators with extensive flow cytometry guidance, from experimental set-up, selection of reagents, setting gating or sorting parameters, data interpretation, and development of new methods. In the previous funding period. Flow Cytometry (previously the major part of the "High Throughput Cell Analysis" Shared Resource) performed key experiments described in at least 83 publications form Cancer Center members. The Resource is widely used, as in the past year, services were provided for 32 Cancer Center members, representing all four Programs. Plans for future development of the Resource include upgrading the current Flow Cytometers, and acquisition of a low-speed microfluidic sorter that can sort much lower numbers of cells and apply substantially lower shear forces than current instruments. This will be particularly useful for small biopsy samples or sorting for rare slow-growing tumor-initiating cells. In general, experiments performed in the Shared Resource are becoming more operator intensive, and the facility has reached the point where a second staff member is needed for sufficient capacity and optimal support for a diverse and growing base of users. Thus, partial salary support is requested for this technician. $109,057 in CCSG support is requested in the first year, representing 27.3% of the total annual operating budget of the Flow Cytometry Shared Resource.

Public Health Relevance

Flow cytometry enables the analysis of the constituents in complex populations of cells, or the sorting and isolation of particular cells that can shed light on the mechanisms of normal and aberrant cell growth. Centralized and shared flow cytometers under expert supervision can efficiently support a wide range of Cancer Center research.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA030199-32
Application #
8473806
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
32
Fiscal Year
2013
Total Cost
$202,036
Indirect Cost
$100,416
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Gong, Xiao-Min; Ding, Yi; Yu, Jinghua et al. (2015) Structure of the Na,K-ATPase regulatory protein FXYD2b in micelles: implications for membrane-water interfacial arginines. Biochim Biophys Acta 1848:299-306
Brun, S N; Markant, S L; Esparza, L A et al. (2015) Survivin as a therapeutic target in Sonic hedgehog-driven medulloblastoma. Oncogene 34:3770-9
You, Weon-Kyoo; Yotsumoto, Fusanori; Sakimura, Kenji et al. (2014) NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function. Angiogenesis 17:61-76
Vargas, Lina M; Leal, Nancy; Estrada, Lisbell D et al. (2014) EphA4 activation of c-Abl mediates synaptic loss and LTP blockade caused by amyloid-? oligomers. PLoS One 9:e92309
Volkmann, Niels; Page, Christopher; Li, Rong et al. (2014) Three-dimensional reconstructions of actin filaments capped by Arp2/3 complex. Eur J Cell Biol 93:179-83
Bailey, Ann M; Zhan, Le; Maru, Dipen et al. (2014) FXR silencing in human colon cancer by DNA methylation and KRAS signaling. Am J Physiol Gastrointest Liver Physiol 306:G48-58
Valencia, Tania; Kim, Ji Young; Abu-Baker, Shadi et al. (2014) Metabolic reprogramming of stromal fibroblasts through p62-mTORC1 signaling promotes inflammation and tumorigenesis. Cancer Cell 26:121-35
Northcott, Paul A; Lee, Catherine; Zichner, Thomas et al. (2014) Enhancer hijacking activates GFI1 family oncogenes in medulloblastoma. Nature 511:428-34
Kim, H; Claps, G; Moller, A et al. (2014) Siah2 regulates tight junction integrity and cell polarity through control of ASPP2 stability. Oncogene 33:2004-10
Finlay, Darren; Vamos, Mitchell; Gonzalez-Lopez, Marcos et al. (2014) Small-molecule IAP antagonists sensitize cancer cells to TRAIL-induced apoptosis: roles of XIAP and cIAPs. Mol Cancer Ther 13:5-15

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