The Flow Cytometry Shared Resource offers Cancer Center users multi-parametric evaluation and high speed enrichment of phenotypically distinct cell populations using flow cytometry. For analytical flow cytometry, investigators have a choice of full-service analysis, or receiving training and then independent use of the two analytical flow cytometers (BD FACSort and BD FACSCanto). Investigator training and consulting is also provided on analytical software including CellQuest, FlowJo, and ModFitLT. For high-speed cell sorting, the facility provides experimental planning and full-service cell sorting by an expert operator, utilizing a BD FACSVantageSE DiVa. This instrument was recently equipped with a custom biosafety cabinet to enable sorting of cells infected with viral vectors, better protecting both the operator and the samples. The experienced Facility Director, Mr. Altman, provides investigators with extensive flow cytometry guidance, from experimental set-up, selection of reagents, setting gating or sorting parameters, data interpretation, and development of new methods. In the previous funding period. Flow Cytometry (previously the major part of the "High Throughput Cell Analysis" Shared Resource) performed key experiments described in at least 83 publications form Cancer Center members. The Resource is widely used, as in the past year, services were provided for 32 Cancer Center members, representing all four Programs. Plans for future development of the Resource include upgrading the current Flow Cytometers, and acquisition of a low-speed microfluidic sorter that can sort much lower numbers of cells and apply substantially lower shear forces than current instruments. This will be particularly useful for small biopsy samples or sorting for rare slow-growing tumor-initiating cells. In general, experiments performed in the Shared Resource are becoming more operator intensive, and the facility has reached the point where a second staff member is needed for sufficient capacity and optimal support for a diverse and growing base of users. Thus, partial salary support is requested for this technician. $109,057 in CCSG support is requested in the first year, representing 27.3% of the total annual operating budget of the Flow Cytometry Shared Resource.

Public Health Relevance

Flow cytometry enables the analysis of the constituents in complex populations of cells, or the sorting and isolation of particular cells that can shed light on the mechanisms of normal and aberrant cell growth. Centralized and shared flow cytometers under expert supervision can efficiently support a wide range of Cancer Center research.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA030199-32
Application #
8473806
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
32
Fiscal Year
2013
Total Cost
$202,036
Indirect Cost
$100,416
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Lechtenberg, Bernhard C; Rajput, Akhil; Sanishvili, Ruslan et al. (2016) Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation. Nature 529:546-50
Zhong, Zhenyu; Umemura, Atsushi; Sanchez-Lopez, Elsa et al. (2016) NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria. Cell 164:896-910
Olson, Erika J; Lechtenberg, Bernhard C; Zhao, Chunxia et al. (2016) Modifications of a Nanomolar Cyclic Peptide Antagonist for the EphA4 Receptor To Achieve High Plasma Stability. ACS Med Chem Lett 7:841-6
Tinoco, Roberto; Carrette, Florent; Barraza, Monique L et al. (2016) PSGL-1 Is an Immune Checkpoint Regulator that Promotes T Cell Exhaustion. Immunity 44:1190-203
Zhao, Wei; Mazar, Joseph; Lee, Bongyong et al. (2016) The Long Noncoding RNA SPRIGHTLY Regulates Cell Proliferation in Primary Human Melanocytes. J Invest Dermatol 136:819-28
Singec, Ilyas; Crain, Andrew M; Hou, Junjie et al. (2016) Quantitative Analysis of Human Pluripotency and Neural Specification by In-Depth (Phospho)Proteomic Profiling. Stem Cell Reports 7:527-42
McQuary, Philip R; Liao, Chen-Yu; Chang, Jessica T et al. (2016) C. elegans S6K Mutants Require a Creatine-Kinase-like Effector for Lifespan Extension. Cell Rep 14:2059-67
Moscat, Jorge; Karin, Michael; Diaz-Meco, Maria T (2016) p62 in Cancer: Signaling Adaptor Beyond Autophagy. Cell 167:606-609
Miletic, Ana V; Jellusova, Julia; Cato, Matthew H et al. (2016) Essential Role for Survivin in the Proliferative Expansion of Progenitor and Mature B Cells. J Immunol 196:2195-204
Koh, Mei Yee; Gagea, Mihai; Sargis, Timothy et al. (2016) A new HIF-1α/RANTES-driven pathway to hepatocellular carcinoma mediated by germline haploinsufficiency of SART1/HAF in mice. Hepatology 63:1576-91

Showing the most recent 10 out of 425 publications