The Pathology Core enables City of Hope Cancer Center investigators to directly study protein and genetic material from human tumors and normal tissues to validate specific hypotheses generated from pre-clinical studies. It also provides analyses of specific protein expression in tumor and normal tissues to enable scientists to discern the distribution patterns of these proteins in specific tissue or cell types. The Pathology Core provides services primarily in the areas of expertise of the Department of Anatomic Pathology. They fall into the following areas: (1) performance and assistance in the routine histological processing of tissues, including paraffin embedding, sectioning, and hematoxylin and eosin (H&E) staining of human tissue, animal tissue, and preparations from cell lines, as well as specialized histological services such as preparation of multi-tumor blocks or tissue arrays to the specifications of the researchers;(2) performance and interpretation of immunohistochemistry for the detection of proteins in normal and tumor tissues, including evaluation and technical development of new antibodies as well as the technology to detect multiple antigens in the same histological section;(3) performance and assistance in Laser Capture Microdissection from paraffin or frozen sections to obtain enrichment of specific cell types, including isolation of malignant cells, for the analysis of proteins, RNA and/or DNA content;(4) assistance in preparing formalin-fixed tissues for conventional PCR and genome sequencing;and (5) consultation services to Cancer Center investigators. Expertise in the pathological basis of disease, the histological manifestations of disease, and the use of human tissues for studying disease states is available for the preparation of grants and papers and in the design of experiments.
The overall goal of the Pathology Core facility is to facilitate research from Cancer Center investigators in animal models and human malignancies by providing access to pathology services and archiving tumors and normal tissues collected from excess surgical materials. The tissue tumor bank includes more than 14,000 samples accumulated over 30 years. This goal enhances the Cancer Center's mission of developing innovative therapeutic strategies in the battle against cancer.
|Kuo, Ching-Ying; Cheng, Chun-Ting; Hou, Peifeng et al. (2016) HIF-1-alpha links mitochondrial perturbation to the dynamic acquisition of breast cancer tumorigenicity. Oncotarget 7:34052-69|
|Nakamura, Ryotaro; La Rosa, Corinna; Longmate, Jeffrey et al. (2016) Viraemia, immunogenicity, and survival outcomes of cytomegalovirus chimeric epitope vaccine supplemented with PF03512676 (CMVPepVax) in allogeneic haemopoietic stem-cell transplantation: randomised phase 1b trial. Lancet Haematol 3:e87-98|
|Wittenberg, Elaine; Ferrell, Betty; Goldsmith, Joy et al. (2016) Family Caregiver Communication Tool: a new measure for tailoring communication with cancer caregivers. Psychooncology :|
|Zhou, Ting; Pan, Feiyan; Cao, Yan et al. (2016) R152C DNA Pol Î² mutation impairs base excision repair and induces cellular transformation. Oncotarget 7:6902-15|
|Sun, Virginia; Ruel, Nora; Chung, Vincent et al. (2016) Pilot study of an interdisciplinary supportive care planning intervention in pancreatic cancer. Support Care Cancer 24:3417-24|
|Li, Zhongqi; Oganesyan, Diana; Mooney, Rachael et al. (2016) L-MYC Expression Maintains Self-Renewal and Prolongs Multipotency ofÂ Primary Human Neural Stem Cells. Stem Cell Reports 7:483-95|
|Reid, Michael A; Lowman, Xazmin H; Pan, Min et al. (2016) IKKÎ² promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. Genes Dev 30:1837-51|
|Leung, Amy; Trac, Candi; Du, Juan et al. (2016) Persistent Chromatin Modifications Induced by High Fat Diet. J Biol Chem 291:10446-55|
|Thomas, Carissa M; Saulnier, Delphine M A; Spinler, Jennifer K et al. (2016) FolC2-mediated folate metabolism contributes to suppression of inflammation by probiotic Lactobacillus reuteri. Microbiologyopen 5:802-818|
|Champer, Jackson; Ito, James I; Clemons, Karl V et al. (2016) Proteomic Analysis of Pathogenic Fungi Reveals Highly Expressed Conserved Cell Wall Proteins. J Fungi (Basel) 2:|
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