The Biomolecular Nuclear Magnetic Resonance (BNMR) Shared Resource provides a broad range of NMRbased services for Huntsman Cancer Institute (HCI) members and the entire University of Utah research community. The Resource provides high-level technical support and access to state-of-the-art NMR software and hardware, including spectrometers operating at 400, 500, 600, 800, and 900 MHz for protein/nucleic acid structure determination as well as routine analytical NMR services. The first three instruments are located at the University of Utah in centralized space and the latter two instruments in Colorado-based regional system (Boulder, 800;Denver, 900). The 400 MHz spectrometer is dedicated to small molecule, organic, and natural products NMR applications. The 500 MHz and 600 MHz spectrometers were recently upgraded to increase BNMR Shared Resource capability. The 600 MHz spectrometer is the highest field spectrometer in Utah. The BNMR Shared Resource manages Sun, SGI, and LINUX workstations for data processing, analysis, and structure determination through the Colorado-based regional system. Commonly used software for NMR spectroscopy is provided, including CYANA2.1, FELIX2004, MOLMOL, NMRPIPE, PYMOL, SPARKY, VNMRJ,andXPLOR-NIH. The BNMR Shared Resource is directed by Jack Skalicky, PhD, a Research Assistant Professor in Biochemistry, who provides technical support for the most demanding NMR projects and assists researchers with data collection, processing, and analysis. The Resource has a full-time NMR technician responsible for training new users, performing scheduled maintenance, and recording small-molecule NMR experiments on the 400 and 500 MHz spectrometers. A shared hardware technician provides repairs. The Resource serves members of all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation;Cell Response and Regulation;Imaging, Diagnostics, and Therapeutics;and Gastrointestinal Cancers) to characterize molecules, particulariy proteins, that play roles in cancer mechanisms. In addition, characterization of small compounds, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The BNMR Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). Use of the BNMR Shared Resource by Cancer Center members with peer-reviewed funding is 60 percent. Funds are requested from the CCSG to cover 16 percent ($42,543) of the proposed Resource budget. The BNMR Shared Resource has ample capacity for additional use by Cancer Center members.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA042014-24
Application #
8465118
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
24
Fiscal Year
2013
Total Cost
$46,253
Indirect Cost
$21,830
Name
University of Utah
Department
Type
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Fuller, Andrew K; Bice, Benjamin D; Venancio, Ashlee R et al. (2018) A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model. J Vis Exp :
Wang, Sophia S; Carrington, Mary; Berndt, Sonja I et al. (2018) HLA Class I and II Diversity Contributes to the Etiologic Heterogeneity of Non-Hodgkin Lymphoma Subtypes. Cancer Res 78:4086-4096
Faham, Najme; Zhao, Ling; Welm, Alana L (2018) mTORC1 is a key mediator of RON-dependent breast cancer metastasis with therapeutic potential. NPJ Breast Cancer 4:36
Rengifo-Cam, William; Shepherd, Hailey M; Jasperson, Kory W et al. (2018) Colon Pathology Characteristics in Li-Fraumeni Syndrome. Clin Gastroenterol Hepatol 16:140-141
Solomon, Benjamin L; Garrido-Laguna, Ignacio (2018) Upper gastrointestinal malignancies in 2017: current perspectives and future approaches. Future Oncol 14:947-962
Gaffney, David K; Hashibe, Mia; Kepka, Deanna et al. (2018) Too many women are dying from cervix cancer: Problems and solutions. Gynecol Oncol 151:547-554
Hardy, Christopher M; Burke, Molly K; Everett, Logan J et al. (2018) Genome-Wide Analysis of Starvation-Selected Drosophila melanogaster-A Genetic Model of Obesity. Mol Biol Evol 35:50-65
Gilcrease, Eddie B; Casjens, Sherwood R (2018) The genome sequence of Escherichia coli tailed phage D6 and the diversity of Enterobacteriales circular plasmid prophages. Virology 515:203-214
Park, Jihye; Blackburn, Brenna E; Rowe, Kerry et al. (2018) Rural-metropolitan disparities in ovarian cancer survival: a statewide population-based study. Ann Epidemiol 28:377-384
Fowler, Brynn; Ding, Qian; Pappas, Lisa et al. (2018) Utah Cancer Survivors: A Comprehensive Comparison of Health-Related Outcomes Between Survivors and Individuals Without a History of Cancer. J Cancer Educ 33:214-221

Showing the most recent 10 out of 1193 publications