The Transgenic Core works with Investigators to generate animal models what will increase our understanding of cancer and the function of genes in involved in cancer. This Core was established in 1989 and produces transgenic mice and rats, and gene-targeted mice (knockouts) for Cancer Center members. The Transgenic Core maintains specialized equipment for micromanipulation, mouse embryonic stem (ES) cell culture, embryo cryopreservation, bacterial artificial chromosome (BAC) recombineering. This collaborative Core combines the expertise of Center members in the molecular biology of Important genes with the Core's expertise in producing genetically engineered mice, Unique capabilities that set this Transgenic Core apart are 1) guaranteed production of transgenic mice and rats. 2) routine production of BAC transgenic mice, 3) production of transgenic mice In unique genetic backgrounds, 4) gene targeting In C57BL/6 ES cell lines in addition to 129/Sv ES cells, 5) de novo derivation of mouse ES cell lines, 6) genetic modification of BACs, 7) open access to reagents and equipment, and 8) training in ES cell culture and micromanipulation. Access to the Transgenic Core eliminates the need for Investigators to purchase specialized equipment and train personnel in embryo micromanipulation, ES cell culture, and BAG recomblneering. Consultation on all aspects of transgenic and ES cell research is provided, from the design of transgenes and conditional targeting vectors to mouse breeding and phenotype analysis. We deliver an average of nine transgenic founder mice and guarantee that at least three founders will be produced for each DNA construct submitted to the Core, The Core electroporates totipotent ES cells with targeting vectors, selects 480 ES cell clones, and provides Investigators with ES cell DNA to screen for homologous recombination with targeting vectors. We guarantee that ES cell clones with desired genetic changes will be microinjected Into at least 60 mouse blastocysts to produce ES cell-mouse chimeras. The efficiency of these procedures meets or exceeds published values in the literature. A full suite of assisted reproductive technologies is offered;mouse cryopreservation, mouse In vitro fertilization, sperm cryopreservation, recovery of mice from cryopreserved embryos or sperm. Center members have taken advantage of these capabilities to establish develop models of gastric cancer, pancreatic cancer, skin cancer, colon cancer, prostate cancer, breast cancer, and medulioblastoma.

Public Health Relevance

Mouse and rat models of human cancer can be produced by genetic engineering. Scientists can use animal models to see what causes cancer and come up with ideas for treatments that will help sick people feel better. When new medicines are invented they can be tested in rats and mice to see if they work before people try them.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee B - Comprehensiveness (NCI)
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University of Michigan Ann Arbor
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Skolarus, Ted A; Metreger, Tabitha; Hwang, Soohyun et al. (2017) Optimizing veteran-centered prostate cancer survivorship care: study protocol for a randomized controlled trial. Trials 18:181
Hertz, Daniel L; Speth, Kelly A; Kidwell, Kelley M et al. (2017) Variable aromatase inhibitor plasma concentrations do not correlate with circulating estrogen concentrations in post-menopausal breast cancer patients. Breast Cancer Res Treat 165:659-668
Pinskey, Justine M; Franks, Nicole E; McMellen, Alexandra N et al. (2017) Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif. J Biol Chem 292:15192-15204
Maj, Tomasz; Wang, Wei; Crespo, Joel et al. (2017) Oxidative stress controls regulatory T cell apoptosis and suppressor activity and PD-L1-blockade resistance in tumor. Nat Immunol 18:1332-1341
Zhang, Jie; Feng, Shumei; Su, Wenmei et al. (2017) Overexpression of FAM83H-AS1 indicates poor patient survival and knockdown impairs cell proliferation and invasion via MET/EGFR signaling in lung cancer. Sci Rep 7:42819
Mann, J E; Hoesli, R; Michmerhuizen, N L et al. (2017) Surveilling the Potential for Precision Medicine-driven PD-1/PD-L1-targeted Therapy in HNSCC. J Cancer 8:332-344
Birkeland, Andrew C; Foltin, Susan K; Michmerhuizen, Nicole L et al. (2017) Correlation of Crtc1/3-Maml2 fusion status, grade and survival in mucoepidermoid carcinoma. Oral Oncol 68:5-8
Walline, Heather M; Goudsmit, Christine M; McHugh, Jonathan B et al. (2017) Integration of high-risk human papillomavirus into cellular cancer-related genes in head and neck cancer cell lines. Head Neck 39:840-852
Walline, Heather M; Carey, Thomas E; Goudsmit, Christine M et al. (2017) High-Risk HPV, Biomarkers, and Outcome in Matched Cohorts of Head and Neck Cancer Patients Positive and Negative for HIV. Mol Cancer Res 15:179-188
Chen, Yan; Zhou, Quan; Li, Xue et al. (2017) Ultrasmall Paramagnetic Iron Oxide Nanoprobe Targeting Epidermal Growth Factor Receptor for In Vivo Magnetic Resonance Imaging of Hepatocellular Carcinoma. Bioconjug Chem 28:2794-2803

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