Microscopy and Imaging - Susette C. Mueller, PhD The objective of the Microscopy and Imaging Shared Resource (MISR) is to provide a supportive environment with state-of-the-art instrumentation for investigators with emphasis on light microscopy and image analysis of tumor cells, including training and education. The MISR also provides a rich environment for interactive exchanges and referrals among Lombardi Comprehensive Cancer Center users. MISR provides instrumentation for bright field microscopy;routine fluorescence microscopy and more advanced applications, including confocal microscopy, microinjection, total internal reflection microscopy (TIRF), multispectral emission imaging, fluorescence lifetime imaging (FLIM), fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), and multiphoton, deep tissue imaging. Live cell, time-lapse imaging is possible using five different wide field and confocal systems. Using Metamorph Offline image analysis, MISR develops automated routines for users carrying out common assays such as for cell tracking, proliferation, mitosis, apoptosis, and angiogenesis experiments. MISR also develops custom journals as requested by users and offers individualized training. The resource also supports cytogenetics and molecular cytogenetics services with expert collaborations directed by Dr. Jan Blancato. Periodic training sessions, open house forums, and seminars are held for users to enhance their expertise in microscopy and provide opportunities to discuss the interpretation of images. Faculty collaborations with MISR provide expertise to others in sophisticated imaging and image analysis approaches. Web-based information and instrument signup calendars enhance use of the shared resource. The MISR provides access to ten major, digital microscope workstations, one stand-alone image analysis workstation and oversees 20 other licenses in individual labs. Since the last submission, MISR added two new sophisticated microscopes: a total internal fluorescence reflection (TIRF) microscope and a confocal/ multiphoton microscope with spectral emission and single photon, fluorescence lifetime imaging capabilities and added a wide field spectral emission camera with software. We plan an upgrade of the fluorescence widefield sceening workstation to include fast, spinning disk confocal, 3D imaging in the spring of 2009. The Microscopy and Imaging Shared Resource (MISR) has been directed by Dr. Susette Mueller since 1991. Dr. Mueller, a tumor cell biologist, became a member of the Lombardi Comprehensive Cancer Center in 1985. Her research on mechanisms of tumor cell invasion and metastasis utilizes a number of microscopic techniques. Dr. Xuehua Xu joined MISR in 2006 with expertise in quantitative dynamic imaging including TIRF, multispectral FRET analysis, and single molecule imaging. Overall, 5 programs and 39 Cancer Center members unfilled this resource in 2008.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA051008-20
Application #
8739829
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-08-15
Project End
2014-04-30
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
20
Fiscal Year
2013
Total Cost
$41,847
Indirect Cost
Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
Bae, Woo Kyun; Yoo, Kyung Hyun; Lee, Ji Shin et al. (2015) The methyltransferase EZH2 is not required for mammary cancer development, although high EZH2 and low H3K27me3 correlate with poor prognosis of ER-positive breast cancers. Mol Carcinog 54:1172-80
Adams, Inez; Christopher, Juleen; Williams, Karen Patricia et al. (2015) What Black Women Know and Want to Know About Counseling and Testing for BRCA1/2. J Cancer Educ 30:344-52
Butrick, Morgan; Kelly, Scott; Peshkin, Beth N et al. (2015) Disparities in uptake of BRCA1/2 genetic testing in a randomized trial of telephone counseling. Genet Med 17:467-75
London, Laricca; Hurtado-de-Mendoza, Alejandra; Song, Minna et al. (2015) Motivators and barriers to Latinas' participation in clinical trials: the role of contextual factors. Contemp Clin Trials 40:74-80
Mays, Darren; Niaura, Raymond S; Evans, W Douglas et al. (2015) Cigarette packaging and health warnings: the impact of plain packaging and message framing on young smokers. Tob Control 24:e87-92
Zhi, Xiuling; Lin, Ling; Yang, Shaoxian et al. (2015) ?II-Spectrin (SPTBN1) suppresses progression of hepatocellular carcinoma and Wnt signaling by regulation of Wnt inhibitor kallistatin. Hepatology 61:598-612
Rush, Christina L; Darling, Margaret; Elliott, Maria Gloria et al. (2015) Engaging Latina cancer survivors, their caregivers, and community partners in a randomized controlled trial: Nueva Vida intervention. Qual Life Res 24:1107-18
Heckler, Mary Mazzotta; Riggins, Rebecca B (2015) ERR? splice variants differentially regulate cell cycle progression. Cell Cycle 14:31-45
Kim, B; Wang, S; Lee, J M et al. (2015) Synthetic lethal screening reveals FGFR as one of the combinatorial targets to overcome resistance to Met-targeted therapy. Oncogene 34:1083-93
Tong, Angie; Kelly, Scott; Nusbaum, Rachel et al. (2015) Intentions for risk-reducing surgery among high-risk women referred for BRCA1/BRCA2 genetic counseling. Psychooncology 24:33-9

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