The Kimmel Cancer Center (KCC) Flow Cytometry Shared Resource (FCSR) provides state-of-the-art cell sorting, phenotypic analysis and related services to the members of the KCC at Thomas Jefferson University (TJU). The facility is located in a dedicated laboratory space on the sixth floor of the Bluemle Life Sciences Building, which is centrally located on the TJU campus. The FCSR operates a fully upgraded Beckman Coulter MoFlo cell sorter capable of 10-color high-speed cell sorting ranging from single cell deposition up to 4-population purification. In 2010 the FCSR purchased a Becton Dickinson LSR 11 analyzer. The LSRll analyzer is equipped with 4 lasers and is capable of 12-color phenotypic analysis. The facility also utilizes a dual laser Becton Dickinson FACSCalibur, which is capable of 4-eolor phenotypic analysis. In addition, the facility offers use of an Invitrogen Countess automated cell counter, one Nikon Labophot-2 fluorescence microscope, and a 4?C refrigerator with -20?C freezer. Data analysis and figure generation are performed on either a Mac Pro workstation with a Dual-Core Intel Xeon processor or a Dell Optiplex 990 with Intel Core 15 processor and the latest version of FlowJo analysis software. An on-line scheduling system facilitates investigators'planning of experiments using analytical cytometers as well as billing and usage tracking. The FCSR is managed by KCC and directed by Dr. Jianke Zhang a faculty and KCC member. All FCSR instruments are maintained and managed by a dedicated facility manager and a technician. The most commonly used applications are cell surface and intracellular phenotyping and sorting, intracellular cytokine and cell signaling molecule detection, analysis of Ca^ mobilization kinetics, apoptosis studies, cell cycle analysis, sorting of rare and stem cell populations, and single cell sorting for cloning assays or monoclonal antibody generation. The FCSR facility plays a critical role in assisting KCC investigators as they conduct research directly related to the understanding and treatment of cancer and other diseases. 39 Cancer Center Members utilize the Flow Cytometry Shared Facilty.
Flow cytometry is an essential research tool for the study of cancer and related diseases. The KCC FCSR facilitates cancer center investigators in their research leading to discovery of novel strategies against cancer and other diseases.
|Ozaki, Shinji; Vuyyuru, Raja; Kageyama, Ken et al. (2016) Establishment and Characterization of Orthotopic Mouse Models for Human Uveal Melanoma Hepatic Colonization. Am J Pathol 186:43-56|
|Teh, Jessica L F; Purwin, Timothy J; Greenawalt, Evan J et al. (2016) An In Vivo Reporter to Quantitatively and Temporally Analyze the Effects of CDK4/6 Inhibitor-Based Therapies in Melanoma. Cancer Res 76:5455-66|
|Zhao, Yongtong; Shapiro, Sandor S; Eto, Masumi (2016) F-actin clustering and cell dysmotility induced by the pathological W148R missense mutation of filamin B at the actin-binding domain. Am J Physiol Cell Physiol 310:C89-98|
|Lu, Huimin; Wang, Tao; Li, Jing et al. (2016) Î±vÎ²6 Integrin Promotes Castrate-Resistant Prostate Cancer through JNK1-Mediated Activation of Androgen Receptor. Cancer Res 76:5163-74|
|Hutcheson, Jack; Balaji, Uthra; Porembka, Matthew R et al. (2016) Immunologic and Metabolic Features of Pancreatic Ductal Adenocarcinoma Define Prognostic Subtypes of Disease. Clin Cancer Res 22:3606-17|
|Singh, Amrita; Fedele, Carmine; Lu, Huimin et al. (2016) Exosome-mediated Transfer of Î±vÎ²3 Integrin from Tumorigenic to Nontumorigenic Cells Promotes a Migratory Phenotype. Mol Cancer Res 14:1136-1146|
|Zhao, Qian; Deng, Shengqiong; Wang, Guangxue et al. (2016) A direct quantification method for measuring plasma MicroRNAs identified potential biomarkers for detecting metastatic breast cancer. Oncotarget 7:21865-74|
|Pattison, Amanda M; Blomain, Erik S; Merlino, Dante J et al. (2016) Intestinal Enteroids Model Guanylate Cyclase C-Dependent Secretion Induced by Heat-Stable Enterotoxins. Infect Immun 84:3083-91|
|Curry, Joseph M; Tassone, Patrick; Cotzia, Paolo et al. (2016) Multicompartment metabolism in papillary thyroid cancer. Laryngoscope 126:2410-8|
|Dowling, John P; Cai, Yubo; Bertin, John et al. (2016) Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation. Cell Death Dis 7:e2379|
Showing the most recent 10 out of 674 publications