The Kimmel Cancer Center (KCC) Flow Cytometry Shared Resource (FCSR) provides state-of-the-art cell sorting, phenotypic analysis and related services to the members of the KCC at Thomas Jefferson University (TJU). The facility is located in a dedicated laboratory space on the sixth floor of the Bluemle Life Sciences Building, which is centrally located on the TJU campus. The FCSR operates a fully upgraded Beckman Coulter MoFlo cell sorter capable of 10-color high-speed cell sorting ranging from single cell deposition up to 4-population purification. In 2010 the FCSR purchased a Becton Dickinson LSR 11 analyzer. The LSRll analyzer is equipped with 4 lasers and is capable of 12-color phenotypic analysis. The facility also utilizes a dual laser Becton Dickinson FACSCalibur, which is capable of 4-eolor phenotypic analysis. In addition, the facility offers use of an Invitrogen Countess automated cell counter, one Nikon Labophot-2 fluorescence microscope, and a 4?C refrigerator with -20?C freezer. Data analysis and figure generation are performed on either a Mac Pro workstation with a Dual-Core Intel Xeon processor or a Dell Optiplex 990 with Intel Core 15 processor and the latest version of FlowJo analysis software. An on-line scheduling system facilitates investigators'planning of experiments using analytical cytometers as well as billing and usage tracking. The FCSR is managed by KCC and directed by Dr. Jianke Zhang a faculty and KCC member. All FCSR instruments are maintained and managed by a dedicated facility manager and a technician. The most commonly used applications are cell surface and intracellular phenotyping and sorting, intracellular cytokine and cell signaling molecule detection, analysis of Ca^ mobilization kinetics, apoptosis studies, cell cycle analysis, sorting of rare and stem cell populations, and single cell sorting for cloning assays or monoclonal antibody generation. The FCSR facility plays a critical role in assisting KCC investigators as they conduct research directly related to the understanding and treatment of cancer and other diseases. 39 Cancer Center Members utilize the Flow Cytometry Shared Facilty.

Public Health Relevance

Flow cytometry is an essential research tool for the study of cancer and related diseases. The KCC FCSR facilitates cancer center investigators in their research leading to discovery of novel strategies against cancer and other diseases.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA056036-15
Application #
8753665
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
15
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
Hussain, Maha; Daignault-Newton, Stephanie; Twardowski, Przemyslaw W et al. (2018) Targeting Androgen Receptor and DNA Repair in Metastatic Castration-Resistant Prostate Cancer: Results From NCI 9012. J Clin Oncol 36:991-999
Shafi, Ayesha A; Schiewer, Matthew J; de Leeuw, Renée et al. (2018) Patient-derived Models Reveal Impact of the Tumor Microenvironment on Therapeutic Response. Eur Urol Oncol 1:325-337
Meyer, Sara E; Muench, David E; Rogers, Andrew M et al. (2018) miR-196b target screen reveals mechanisms maintaining leukemia stemness with therapeutic potential. J Exp Med 215:2115-2136
Mazina, Olga M; Mazin, Alexander V (2018) Reconstituting the 4-Strand DNA Strand Exchange. Methods Enzymol 600:285-305
Magee, Michael S; Abraham, Tara S; Baybutt, Trevor R et al. (2018) Human GUCY2C-Targeted Chimeric Antigen Receptor (CAR)-Expressing T Cells Eliminate Colorectal Cancer Metastases. Cancer Immunol Res 6:509-516
Chervoneva, Inna; Freydin, Boris; Hyslop, Terry et al. (2018) Modeling qRT-PCR dynamics with application to cancer biomarker quantification. Stat Methods Med Res 27:2581-2595
Capparelli, Claudia; Purwin, Timothy J; Heilman, Shea A et al. (2018) ErbB3 Targeting Enhances the Effects of MEK Inhibitor in Wild-Type BRAF/NRAS Melanoma. Cancer Res 78:5680-5693
Nevler, Avinoam; Muller, Alexander J; Cozzitorto, Joseph A et al. (2018) A Sub-Type of Familial Pancreatic Cancer: Evidence and Implications of Loss-of-Function Polymorphisms in Indoleamine-2,3-Dioxygenase-2. J Am Coll Surg 226:596-603
Peng, Weidan; Furuuchi, Narumi; Aslanukova, Ludmila et al. (2018) Elevated HuR in Pancreas Promotes a Pancreatitis-Like Inflammatory Microenvironment That Facilitates Tumor Development. Mol Cell Biol 38:
Waldman, Scott A; Camilleri, Michael (2018) Guanylate cyclase-C as a therapeutic target in gastrointestinal disorders. Gut 67:1543-1552

Showing the most recent 10 out of 807 publications