State-of-the-art imaging technologies are powerful tools that can be leveraged to study various cellular and molecular mechanisms in cancer progression and how tumor cells interact with the tumor microenvironment. The Cell Imaging Facility provides imaging modalities that cover the range from single molecule microscopy to whole animals, and from brightfield, to fluorescence and bioluminescence imaging. The Cell Imaging Facility offers bioluminescene/fluorescence in vivo imagers, microprobe lens-driven intravital microscope, multiphoton microscope, various fluorescence microscopes including macroview stereoscope, high content slide scanner and tissue cytometer, spinning disk confocal microscope, multispectral confocals with resonant scanner, integrated incubator microscope, structured illumination super-resolution microscope, laser microdissection system, and transmission electron microscope with 3D tomography capability. Collectively, this vast instrument portfolio offers core services in advanced microscopy on single molecules, macromolecular networks, live cells, FRET, FRAP, FLIP, photoconversion, TIRF, large tissue sections in bright field and fluorescence, specific tissue procurement, intravital imaging, and whole animal imaging. This creates a unique environment in which investigators can design workflow-based correlative experiments to study their cancer specimens all the way from whole animals to single molecules. This wide-ranging microscopy instrumentation and services is fully supported by the technical expertise of a team that has been consecutively recognized by the Northwestern University Office for Research as Outstanding Core for multiple years. The team is led by the facility director. Dr. Teng-Leong Chew. Dr. Chew is an expert in advanced live cell imaging, and has pioneered the development of several biosensors and imaging applications. Together the team provides active training and educational outreach in personalized instrument training, experimental consultation, seminars, journal clubs, hands-on workshops, as well as image analysis and programming boot camps. This 24-hr facility serves more than 180 labs throughout the university, and we maintain our leadership position in novel imaging technologies through a two-pronged approach: (i) aggressive and successful effort in securing shared instrument funds. We have added ten instruments over $300K since the last CCSG cycle;(ii) leveraging our expertise to attract leading manufacturers to establish beta test sites in the imaging center. These efforts have culminated in the facility being recognized by Nikon as one of only eight Nikon Imaging Centers in the world, allowing the cancer center to benefit immensely from the investment of Nikon's latest technologies. In 2012, we were selected by EuroBioimaging Consortium as the first American imaging center to join this trans-Atlantic multinational imaging network. These achievements place the Cancer Center uniquely at the forefront of cancer imaging technologies.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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Northwestern University at Chicago
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Yang, Ruiguo; LemaƮtre, Vincent; Huang, Changjin et al. (2018) Monoclonal Cell Line Generation and CRISPR/Cas9 Manipulation via Single-Cell Electroporation. Small 14:e1702495
Buglak, Nicholas E; Jiang, Wulin; Bahnson, Edward S M (2018) Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats. Redox Biol 19:166-178
Takahashi, Satoe; Sun, Willy; Zhou, Yingjie et al. (2018) Prestin Contributes to Membrane Compartmentalization and Is Required for Normal Innervation of Outer Hair Cells. Front Cell Neurosci 12:211
Zheng, Jianbin; Chen, Long; Skinner, Owen S et al. (2018) ?-Glucocerebrosidase Modulators Promote Dimerization of ?-Glucocerebrosidase and Reveal an Allosteric Binding Site. J Am Chem Soc 140:5914-5924
Kenney, Grace E; Dassama, Laura M K; Pandelia, Maria-Eirini et al. (2018) The biosynthesis of methanobactin. Science 359:1411-1416
Joyce, Brian T; Zheng, Yinan; Zhang, Zhou et al. (2018) miRNA-Processing Gene Methylation and Cancer Risk. Cancer Epidemiol Biomarkers Prev 27:550-557
Chu, Lan H; Indramohan, Mohanalaxmi; Ratsimandresy, Rojo A et al. (2018) The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun 9:996
Symes, Yael R; Barrington, Clare; Austin, Jane et al. (2018) Advice to patients undergoing stem cell transplant: Content analysis of survivor peer support narratives. J Health Psychol 23:818-828
Lewis, Phillip L; Green, Richard M; Shah, Ramille N (2018) 3D-printed gelatin scaffolds of differing pore geometry modulate hepatocyte function and gene expression. Acta Biomater 69:63-70
Ugolkov, Andrey V; Bondarenko, Gennadiy I; Dubrovskyi, Oleksii et al. (2018) 9-ING-41, a small-molecule glycogen synthase kinase-3 inhibitor, is active in neuroblastoma. Anticancer Drugs 29:717-724

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