Protein identification and proteomics are important new tools in cancer research. The Protein Mass Spectrometry Shared Resource will provide high-level mass spectrometry of biomolecules and protein analyses. This Shared Resource is based around a pair of tandem mass analyzers (the AB 4700 MALDITOF/ TOF and Thermo LTQ nanoESI-QIT), with access to as many as ten HPLC systems containing 10 quaternary and two binary pumps, seven PCs running at least 21 software packages, and numerous smaller items. The Facility is able to routinely identify proteins in slices from polyacryamide gels. In addition, it can routinely identify proteins in pull-down mixtures, complex virions, and whole cell extracts. They have done multi-dimensional (up to three dimensions) of tandem HPLC prior to LC-MALDI analysis. This capability includes two-dimensional protein fractionation (PF2D) and two and three dimensions of tandem peptide fractionation. This Facility provides an exciting springboard to the frontiers of state-of-the art proteomics. The Protein Mass Spectrometry Facility has been supported by the Cancer Center as a developing Shared Resource for the past three years. Permanent funds are now requested for support as a full Shared Resource.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA062203-18
Application #
8740838
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-09-11
Project End
2014-01-31
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
18
Fiscal Year
2013
Total Cost
$47,611
Indirect Cost
$16,503
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Cox-Muranami, Wesley A; Nelson, Edward L; Li, G P et al. (2016) Large area magnetic micropallet arrays for cell colony sorting. Lab Chip 16:172-81
Klempner, Samuel J; Gershenhorn, Bruce; Tran, Phu et al. (2016) BRAFV600E Mutations in High-Grade Colorectal Neuroendocrine Tumors May Predict Responsiveness to BRAF-MEK Combination Therapy. Cancer Discov 6:594-600
Li, N; Pasricha, S; Bulsiewicz, W J et al. (2016) Effects of preceding endoscopic mucosal resection on the efficacy and safety of radiofrequency ablation for treatment of Barrett's esophagus: results from the United States Radiofrequency Ablation Registry. Dis Esophagus 29:537-43
Nolen, Shantell C; Lee, Brian; Shantharam, Shruti et al. (2016) The effects of sequential treatments on hippocampal volumes in malignant glioma patients. J Neurooncol 129:433-41
Bota, Daniela A; Davies, Kelvin J A (2016) Mitochondrial Lon protease in human disease and aging: Including an etiologic classification of Lon-related diseases and disorders. Free Radic Biol Med 100:188-198
Pedram, Ali; Razandi, Mahnaz; Blumberg, Bruce et al. (2016) Membrane and nuclear estrogen receptor α collaborate to suppress adipogenesis but not triglyceride content. FASEB J 30:230-40
Greene, Christina L; Worrell, Stephanie G; Attwood, Stephen E et al. (2016) Emerging Concepts for the Endoscopic Management of Superficial Esophageal Adenocarcinoma. J Gastrointest Surg 20:851-60
Iyer, Neel S; Osann, Kathryn; Hsieh, Susie et al. (2016) Health Behaviors in Cervical Cancer Survivors and Associations with Quality of Life. Clin Ther 38:467-75
Leproux, Anaïs; Kim, You Me; Min, Jun Won et al. (2016) Differential diagnosis of breast masses in South Korean premenopausal women using diffuse optical spectroscopic imaging. J Biomed Opt 21:74001
McLaren, Christine E; Barton, James C; Subramaniam, V Nathan et al. (2016) Reply. Hepatology 63:2058-60

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