Abstract

Protein identification and proteomics are important new tools in cancer research. The Protein Mass Spectrometry Shared Resource will provide high-level mass spectrometry of biomolecules and protein analyses. This Shared Resource is based around a pair of tandem mass analyzers (the AB 4700 MALDITOF/ TOF and Thermo LTQ nanoESI-QIT), with access to as many as ten HPLC systems containing 10 quaternary and two binary pumps, seven PCs running at least 21 software packages, and numerous smaller items. The Facility is able to routinely identify proteins in slices from polyacryamide gels. In addition, it can routinely identify proteins in pull-down mixtures, complex virions, and whole cell extracts. They have done multi-dimensional (up to three dimensions) of tandem HPLC prior to LC-MALDI analysis. This capability includes two-dimensional protein fractionation (PF2D) and two and three dimensions of tandem peptide fractionation. This Facility provides an exciting springboard to the frontiers of state-of-the art proteomics. The Protein Mass Spectrometry Facility has been supported by the Cancer Center as a developing Shared Resource for the past three years. Permanent funds are now requested for support as a full Shared Resource.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA062203-18
Application #
8740838
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-09-11
Project End
2014-01-31
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
18
Fiscal Year
2013
Total Cost
$47,611
Indirect Cost
$16,503

  Institution

Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Kim, Seong M; Nguyen, Tricia T; Ravi, Archna et al. (2018) PTEN Deficiency and AMPK Activation Promote Nutrient Scavenging and Anabolism in Prostate Cancer Cells. Cancer Discov 8:866-883
Qiu, Xiaolong; Huang, Jen-Huang; Westerhof, Trisha M et al. (2018) Microfluidic channel optimization to improve hydrodynamic dissociation of cell aggregates and tissue. Sci Rep 8:2774
Zhu, Yong; Wang, Xiuye; Forouzmand, Elmira et al. (2018) Molecular Mechanisms for CFIm-Mediated Regulation of mRNA Alternative Polyadenylation. Mol Cell 69:62-74.e4
Mishra, Birendra; Lawson, Gregory W; Ripperdan, Ryan et al. (2018) Charged-Iron-Particles Found in Galactic Cosmic Rays are Potent Inducers of Epithelial Ovarian Tumors. Radiat Res 190:142-150
Song, Wan; Zsindely, Nóra; Faragó, Anikó et al. (2018) Systematic genetic interaction studies identify histone demethylase Utx as potential target for ameliorating Huntington's disease. Hum Mol Genet 27:649-666
Lin, Xiaoxiao; Itoga, Christy A; Taha, Sharif et al. (2018) c-Fos mapping of brain regions activated by multi-modal and electric foot shock stress. Neurobiol Stress 8:92-102
Kim, Chang Soo; Ingato, Dominique; Wilder-Smith, Petra et al. (2018) Stimuli-disassembling gold nanoclusters for diagnosis of early stage oral cancer by optical coherence tomography. Nano Converg 5:3
Chakraborty, Mahul; VanKuren, Nicholas W; Zhao, Roy et al. (2018) Hidden genetic variation shapes the structure of functional elements in Drosophila. Nat Genet 50:20-25
Morozko, Eva L; Ochaba, Joseph; Hernandez, Sarah J et al. (2018) Longitudinal Biochemical Assay Analysis of Mutant Huntingtin Exon 1 Protein in R6/2 Mice. J Huntingtons Dis 7:321-335
Carpenter, Philip M; Ziogas, Argyrios; Markham, Emma M et al. (2018) Laminin 332 expression and prognosis in breast cancer. Hum Pathol 82:289-296

Showing the most recent 10 out of 1106 publications