The Molecular Recognition Shared Resource (MRSR) is a newly established core facility. The goal of the MRSR is to provide investigators with reagents, equipment, and expertise for the production, detection and characterization of biomolecules. The MRSR is composed of three individual Resources , the Antibody Resource, the Protein Expression Resource and the BIAcore Resource. Each of these resources provides unique, cost- effective services and expertise for Vanderbilt Cancer Center (VCC) and University Investigators. The goals and the objectives of three resources are described below. The goal of the Antibody Resource is to provide the expertise, reagents and equipment for the production, detection, characterization, purification and labeling of traditional polyclonal, monoclonal and phage-displayed or soluble ScFv recombinant antibodies. Assays incorporating antibodies can also be developed to detect and/or quantitate proteins, carbohydrates or nucleic acids which occur individually or as components of a biological milieu. The objective of the Antibody Resource is to provide antibodies and antibody-based assays to VCC and University Investigators on a timely and cost-effective basis. The goal of the Protein Expression Resource is to provide VCC and University Investigators with reagents and equipment for large scale protein expression using the baculovirus expression system. Additionally, Investigators are provided with equipment necessary to harvest large amounts of cellular material. The goal and objective of the BIAcore Resource is to provide VCC and University Investigators with access to the BIAcore 2000, an instrument from BIAcore, AB., that performs real-time biomolecular interaction analysis using surface plasmon resonance technology.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA068485-05S2
Application #
6398978
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2000
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37203
Gilchuk, Pavlo; Kuzmina, Natalia; Ilinykh, Philipp A et al. (2018) Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein. Immunity 49:363-374.e10
Hebron, Katie E; Li, Elizabeth Y; Arnold Egloff, Shanna A et al. (2018) Alternative splicing of ALCAM enables tunable regulation of cell-cell adhesion through differential proteolysis. Sci Rep 8:3208
Bangaru, Sandhya; Zhang, Heng; Gilchuk, Iuliia M et al. (2018) A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA. Nat Commun 9:2669
Means, Anna L; Freeman, Tanner J; Zhu, Jing et al. (2018) Epithelial Smad4 Deletion Up-Regulates Inflammation and Promotes Inflammation-Associated Cancer. Cell Mol Gastroenterol Hepatol 6:257-276
Du, Zhenfang; Lovly, Christine M (2018) Mechanisms of receptor tyrosine kinase activation in cancer. Mol Cancer 17:58
Zhang, Qin; Jeppesen, Dennis K; Higginbotham, James N et al. (2018) Mutant KRAS Exosomes Alter the Metabolic State of Recipient Colonic Epithelial Cells. Cell Mol Gastroenterol Hepatol 5:627-629.e6
Elion, David L; Cook, Rebecca S (2018) Harnessing RIG-I and intrinsic immunity in the tumor microenvironment for therapeutic cancer treatment. Oncotarget 9:29007-29017
Kook, Seunghyi; Qi, Aidong; Wang, Ping et al. (2018) Gene-edited MLE-15 Cells as a Model for the Hermansky-Pudlak Syndromes. Am J Respir Cell Mol Biol 58:566-574
Choksi, Yash A; Reddy, Vishruth K; Singh, Kshipra et al. (2018) BVES is required for maintenance of colonic epithelial integrity in experimental colitis by modifying intestinal permeability. Mucosal Immunol 11:1363-1374
Cooke, Allison L; Morris, Jamie; Melchior, John T et al. (2018) A thumbwheel mechanism for APOA1 activation of LCAT activity in HDL. J Lipid Res 59:1244-1255

Showing the most recent 10 out of 2462 publications