In accordance with our strategic plan and endorsed by our external advisors, the former Mouse Models Core has expanded to offer multiple imaging modalities and thus reorganized into the Small Animal Modeling and Imaging (SAMI) Core to reflect this improved, integrated focus. The primary purpose of the SAMI core is to provide proficient generation and examination of rodent models of cancer for basic and translational research. SAMI continues to provide specialized consultations and services for the derivation, characterization, and preservation of genetically-engineered mice, and a variety of tumor cell lines for xenograft and syngeneic transplant mouse models. Tumor development, progression, metastasis, and response to therapy in transgenic or transplanted mouse models of cancer can be evaluated using modern multimodality imaging including bioluminescent and biofluorescent imaging, ultrasound, magnetic resonance imaging, and beta particle imaging. Within the last funding period, the core has experienced significant growth, including the addition of imaging sen/ices, expert personnel, a new Scientific Director, acquisition of new lab space and capital equipment, and provision of several new services including an MRI, an ultrasound, bioluminescence and biofluorescence, beta-imaging, intravital microscopy, cryopreservation and rederivation, and ES cell culture. The Core requests CCSG Suppori of $17S,286, which is S1% of its operational budget. Over 80% of users were Moffitt members and peer-reviewed. Usage increased in all services. The core derived 66 founders or chimeras for 12 genetically engineered mouse models and cryopreserved or rederived 8 mouse lines. Genomic DNA was prepared from 774 mouse tail biopsies and genotyped via 20 hybridization blots. Twelve luciferase-expressing tumor cell lines were distributed for use in xenograft mouse models. In FY 2010, the Caliper IVIS workstations were utilized for 519 hours by 15 members;4S4 hours on the IVIS-200 and 85 hours on the IVIS-100. The Visual Sonics Vevo 2100 US system was used for 165 hours by six Pis. The Varian 7T MRI instrument was used for 486 hours by five investigators.

Public Health Relevance

The SAMI core provides proficient generation and examination of rodent models and tumor development, progression, metastasis, and response to therapy in transgenic or transplanted mouse models of cancer can be evaluated using modern multimodality imaging including bioluminescent and biofluorescent imaging, ultrasound, magnetic resonance imaging, and beta particle imaging.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA076292-15
Application #
8495987
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
15
Fiscal Year
2013
Total Cost
$97,989
Indirect Cost
$39,835
Name
H. Lee Moffitt Cancer Center & Research Institute
Department
Type
DUNS #
139301956
City
Tampa
State
FL
Country
United States
Zip Code
33612
Davis, Stacy N; Govindaraju, Swapamthi; Jackson, Brittany et al. (2018) Recruitment Techniques and Strategies in a Community-Based Colorectal Cancer Screening Study of Men and Women of African Ancestry. Nurs Res 67:212-221
Martínez, Úrsula; Brandon, Thomas H; Sutton, Steven K et al. (2018) Associations between the smoking-relatedness of a cancer type, cessation attitudes and beliefs, and future abstinence among recent quitters. Psychooncology 27:2104-2110
Perales-Puchalt, Alfredo; Perez-Sanz, Jairo; Payne, Kyle K et al. (2018) Frontline Science: Microbiota reconstitution restores intestinal integrity after cisplatin therapy. J Leukoc Biol 103:799-805
Nelson, Ashley M; Jim, Heather S L; Small, Brent J et al. (2018) Sleep disruption among cancer patients following autologous hematopoietic cell transplantation. Bone Marrow Transplant 53:307-314
Singh, Kshipra; Coburn, Lori A; Asim, Mohammad et al. (2018) Ornithine Decarboxylase in Macrophages Exacerbates Colitis and Promotes Colitis-Associated Colon Carcinogenesis by Impairing M1 Immune Responses. Cancer Res 78:4303-4315
Kasting, Monica L; Giuliano, Anna R; Reich, Richard R et al. (2018) Hepatitis C Virus Screening Trends: Serial Cross-Sectional Analysis of the National Health Interview Survey Population, 2013-2015. Cancer Epidemiol Biomarkers Prev 27:503-513
Denson, Aaron; Burke, Nancy; Wapinsky, Georgine et al. (2018) Clinical Outcomes of Patients With Gastrointestinal Malignancies Participating in Phase I Clinical Trials. Am J Clin Oncol 41:133-139
Betts, Brian C; Bastian, David; Iamsawat, Supinya et al. (2018) Targeting JAK2 reduces GVHD and xenograft rejection through regulation of T cell differentiation. Proc Natl Acad Sci U S A 115:1582-1587
Pidala, Joseph; Beato, Francisca; Kim, Jongphil et al. (2018) In vivo IL-12/IL-23p40 neutralization blocks Th1/Th17 response after allogeneic hematopoietic cell transplantation. Haematologica 103:531-539
Hampras, S S; Tommasino, M; Zhao, Y et al. (2018) Cross-sectional associations between cutaneous viral infections and regulatory T lymphocytes in circulation. Br J Dermatol :

Showing the most recent 10 out of 1254 publications