9.6.1 ABSTRACT: MOUSE GENETICS LABORATORY Director: David Largaespada, Ph.D. The Mouse Genetics Laboratory (MGL) shared resource provides cancer center members with access to stateof- the-art technologies required to create and efficiently study genetically modified mice. Genetically modified mice have been vital tools for cancer studies for many years, allowing one to determine the role of specific genes in cancer-relevant traits such as the immune responses, tumor initiation, and metastasis to name a few. Therefore, access to these technologies is critical to the ongoing work of the University of Minnesota Cancer Center. Cancer Center members have used this shared resource to create mouse models of myeloid leukemia, multiple myeloma, colorectal cancer and infant leukemia. Transgenic mice produced by this shared resource have been used to create a system for performing forward genetic screens for cancer genes in mice. Members have studied aspects of T and B cell development and immunity using transgehic mice created by this shared resource. These studies have relevance for understanding tumor surveillance and methods to break tolerance for anti-tumor immunity. We anticipate that this shared resource will continue to serve investigators that require in vivo genetic confirmation of important hypotheses touching many aspects of cancer biology. The MGL has served the Cancer Center for 10 years, indeed since the first Cancer Center Support Grant (CCSG) application. The MGL provides many interrelated services that require manipulation of mouse embryos or embryonic stem (ES) cells. We perform pronuclear microinjection for the creation of transgenic mice, blastocyst injection for projects that involve genetic manipulation of ES cells, in vitro fertilization, sperm/embryo cyropreservation, and any service that requires manipulation of early mouse embryos. We recently added another service, which is ES cell gene targeting and expansion. The MGL provides free ES cells, drugresistant mouse embryo fibroblasts (MEFs), and plasmid vectors. Finally, we provide free consultation on mouse husbandry and strain maintenance, the design of ES cell gene targeting experiments, and the use of mouse models for cancer-related research in general. The MGL shared resource leader is Dr. David Largaespada, an Associate Professor in the Department of Genetics, Cell Biology and Development. He has been this shared resource's leader or co-leader since the beginning of the MGL and sets priorities for scientific use of the MGL in consultation with Cancer Center member users and the Rodent Advisory Committee (RAC) of the University of Minnesota. The MGL currently consists of three full time staff members in addition to Dr. Largaespada.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Minnesota Twin Cities
United States
Zip Code
Abbott, Kenneth L; Nyre, Erik T; Abrahante, Juan et al. (2015) The Candidate Cancer Gene Database: a database of cancer driver genes from forward genetic screens in mice. Nucleic Acids Res 43:D844-8
Yee, Douglas (2015) A tale of two receptors: insulin and insulin-like growth factor signaling in cancer. Clin Cancer Res 21:667-9
Daniel, A R; Gaviglio, A L; Knutson, T P et al. (2015) Progesterone receptor-B enhances estrogen responsiveness of breast cancer cells via scaffolding PELP1- and estrogen receptor-containing transcription complexes. Oncogene 34:506-15
Upadhyaya, Pramod; Hecht, Stephen S (2015) Quantitative analysis of 3'-hydroxynorcotinine in human urine. Nicotine Tob Res 17:524-9
Patel, Yesha M; Stram, Daniel O; Wilkens, Lynne R et al. (2015) The contribution of common genetic variation to nicotine and cotinine glucuronidation in multiple ethnic/racial populations. Cancer Epidemiol Biomarkers Prev 24:119-27
Takahashi, Yutaka; Hui, Susanta K (2014) Fast, simple, and informative patient-specific dose verification method for intensity modulated total marrow irradiation with helical tomotherapy. Radiat Oncol 9:34
Cooley, Sarah; Weisdorf, Daniel J; Guethlein, Lisbeth A et al. (2014) Donor killer cell Ig-like receptor B haplotypes, recipient HLA-C1, and HLA-C mismatch enhance the clinical benefit of unrelated transplantation for acute myelogenous leukemia. J Immunol 192:4592-600
Chen, Liddy M; Ibrahim, Joseph G; Chu, Haitao (2014) Flexible stopping boundaries when changing primary endpoints after unblinded interim analyses. J Biopharm Stat 24:817-33
Landman, Sean R; Hwang, Tae Hyun; Silverstein, Kevin A T et al. (2014) SHEAR: sample heterogeneity estimation and assembly by reference. BMC Genomics 15:84
Gates, Leah A; Phillips, Martin B; Matter, Brock A et al. (2014) Comparative metabolism of furan in rodent and human cryopreserved hepatocytes. Drug Metab Dispos 42:1132-6

Showing the most recent 10 out of 319 publications