The Optical Biology Shared Resource (Opticore) provides access to state-of-the-art flow cytometry, cell sorting, and laser scanning cytometry for the UC Davis Cancer Center community. The core facility is located on the Davis campus with two satellites in Sacramento. The objectives of this shared resource are to: provide state-of-the-art cell sorting and analytical cytometry;provide consultation about appropriate experimental design and data analysis;provide education about routine as well as cutting-edge cytometry applications;? facilitate interactions among researchers using these techniques;and stimulate development of new approaches for cancer research. This shared resource provides cost-effective services and training. Investigators and their laboratory members work closely with the Technical Director to learn to use multicolor benchtop analyzers. The staff teaches an annual summer flow cytometry course for new users. Multi-laser, high-speed cell sorters are available on both campuses and are operated by Opticore staff. A biohazard containment system permits sorting of unfixed cells. The 3-laser scanning cytometer is configured for multiparameter and high-throughput applications, including analysis of tissue microarrays. These instruments have been upgraded with institutional support and are maintained through ongoing service contracts. The Cancer Center administration has designed and implemented a web-based reservation and reporting system. Dr. Barbara Shacklett is the Scientific Director;the Technical Director, Ms. Oxford, has developed notable expertise in multicolor analysis and high speed sorting. She is assisted by a Technical Manager, Ms. McLaughlin, who supervises operations in Sacramento. An Advisory Committee meets at least once a year to review operations, set policy, and make plans. Since the last submission, the user pool has expanded and Cancer Center members from all six research programs have used this shared resource. Members currently account for over 80% of its use, generating results published in high-impact journals and submitted in grant applications.

Public Health Relevance

The Optical Biology Shared Resource (Opticore) provides access to state-of-the-art flow cytometry, cell sorting, and laser scanning cytometry for the UC Davis Cancer Center research community. This technology is widely used in cancer research to evaluate cells and cell properties, and thus contributes to improved cancer research, and ultimately to better cancer therapies.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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University of California Davis
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Semrad, Thomas; Barzi, Afsaneh; Lenz, Heinz-Josef et al. (2015) Pharmacodynamic separation of gemcitabine and erlotinib in locally advanced or metastatic pancreatic cancer: therapeutic and biomarker results. Int J Clin Oncol 20:518-24
Brostoff, Terza; Dela Cruz Jr, Florante N; Church, Molly E et al. (2014) The raccoon polyomavirus genome and tumor antigen transcription are stable and abundant in neuroglial tumors. J Virol 88:12816-24
Kirschbaum, Mark H; Foon, Kenneth A; Frankel, Paul et al. (2014) A phase 2 study of belinostat (PXD101) in patients with relapsed or refractory acute myeloid leukemia or patients over the age of 60 with newly diagnosed acute myeloid leukemia: a California Cancer Consortium Study. Leuk Lymphoma 55:2301-4
Mayadev, Jyoti; Qi, Lihong; Lentz, Susan et al. (2014) Implant time and process efficiency for CT-guided high-dose-rate brachytherapy for cervical cancer. Brachytherapy 13:233-9
Daly, Megan E; Beckett, Laurel A; Chen, Allen M (2014) Does early posttreatment surveillance imaging affect subsequent management following stereotactic body radiation therapy for early-stage non-small cell lung cancer? Pract Radiat Oncol 4:240-6
Li, Tianhong; Maus, Martin K H; Desai, Sonal J et al. (2014) Large-scale screening and molecular characterization of EML4-ALK fusion variants in archival non-small-cell lung cancer tumor specimens using quantitative reverse transcription polymerase chain reaction assays. J Thorac Oncol 9:18-25
Campbell, Mel; Kim, Kevin Y; Chang, Pei-Ching et al. (2014) A lytic viral long noncoding RNA modulates the function of a latent protein. J Virol 88:1843-8
Li, Tianhong; Kung, Hsing-Jien; Mack, Philip C et al. (2013) Genotyping and genomic profiling of non-small-cell lung cancer: implications for current and future therapies. J Clin Oncol 31:1039-49
Semrad, Thomas J; Eddings, Courtney; Dutia, Mrinal P et al. (2013) Phase I study of the combination of temsirolimus and pazopanib in advanced solid tumors. Anticancer Drugs 24:636-40
Maus, Martin K H; Mack, Philip C; Astrow, Stephanie H et al. (2013) Histology-related associations of ERCC1, RRM1, and TS biomarkers in patients with non-small-cell lung cancer: implications for therapy. J Thorac Oncol 8:582-6

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