The Genomics Shared Service (GSS) Is a comprehensive resource for supporting genomic studies to investigators at the University of Maryland School of Medicine, including the Marlene and Stewart Greenebaum Cancer Center (UMGCC). Services include DNA sequencing, gene expression studies, genotyping, and high-throughput DNA sequencing. GSS provides access to the informatics resources necessary to provide a seamless pathway from experimental planning and initial analysis of raw data to advanced analysis in a biological context. GSS comprises two genomic-related core laboratories supported by a third resource, the informatics resource center. Instrumentation operated by the GSS is state-of-the-art. DNA sequencing services are supported by six Applied Biosystems model 3730XL automated sequencers. High-throughput DNA sequencing is performed on two Roche 454 FLX pyrosequencers, an lllumina Genome Analyzer IIX, and an lllumina HiSeq2000. Genotyping and gene expression services are supported by an Applied Biosystems model 7900 HT Fast real-time PCR system, and lllumina BeadXpress, and Affymetrix GeneChip system 3000 7G, and an lllumina iScan. Highly skilled informatics faculty and staff provide investigators at the UMGCC with the expertise to analyze multidimensional datasets, using existing software tools, or to develop new, novel software and algorithms as necessary. A key strength of the GSS is the educational opportunities provided to students (graduate and medical) and fellows (postdoctoral and clinical) as well as faculty. These educational opportunities have a profound impact on the success the GSS has in transforming cancer research programs. The effectiveness of this shared service can be measured by the large number of publications supported annually?over 100 publications per year from members of the UMGCC alone. Many of these publications are in high-impact journals, including Cell, Proceedings of the National Academy of Sciences, Science, Nature Genetics, Cancer Research, Biochemistry, and the Journal of Molecular Biology.

Public Health Relevance

The application of genomic technology to cancer research is essential in determining the molecular mechanisms involved in cancer etiology. The field of genomics will undoubtedly play a critical role in detection, treatment, and ultimately prevention of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA134274-05
Application #
8379051
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$114,354
Indirect Cost
$30,306
Name
University of Maryland Baltimore
Department
Type
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201
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Kochel, Tyler J; Fulton, Amy M (2015) Multiple drug resistance-associated protein 4 (MRP4), prostaglandin transporter (PGT), and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) as determinants of PGE2 levels in cancer. Prostaglandins Other Lipid Mediat 116-117:99-103
Rasmussen, Jacob H; Vogelius, Ivan R; Aznar, Marianne C et al. (2015) Spatio-temporal stability of pre-treatment 18F-Fludeoxyglucose uptake in head and neck squamous cell carcinomas sufficient for dose painting. Acta Oncol 54:1416-22
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