The High Throughput Screening Shared Resource allows members of the Cancer Center to use the techniques of high-throughput screening (HTS) of compounds or genomic siRNA libraries. The HTS laboratory provides target assessment and assay evaluation, assay development and testing, screening of chemical compounds or siRNA libraries, data analysis and storage, and "hit" validation. To gain access to the HTS facility, Cancer Center members must have a project evaluated by the Cancer Center HTS Oversight Committee, which will assess both the practicality and the scientific and therapeutic value of targets for screening. For approved projects, the HTS staff will then assess the cost and practicality of the proposed assay and work with the initiating laboratory to develop the assay into a form suitable for HTS that reproducibly achieves the desired signal to noise statistics. The HTS laboratory works to minimize the cost of the experiment, which often requires changing reagents and sometimes completely redesigning the assay. Once an assay has an acceptable signal to noise ratio in small scale experiments, for compound screens multiple assay plates are treated with DMSO (the compound diluent) to determine reproducibility under conditions similar to HTS screening and then are screened against a test compound library to determine the "hit" rate. Genomic siRNA experiments are developed in a similar manner, except that they are run in 96 well format in replicate plates and no test library is used before the genomic screen is begun. Just as with compounds, siRNA experimental controls must achieve a reproducible signal to background ratio in runs of multiple plates before the project is allowed to screen the genomic library.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA142543-04
Application #
8519961
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
4
Fiscal Year
2013
Total Cost
$73,129
Indirect Cost
$27,467
Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Huang, Xiumei; Motea, Edward A; Moore, Zachary R et al. (2016) Leveraging an NQO1 Bioactivatable Drug for Tumor-Selective Use of Poly(ADP-ribose) Polymerase Inhibitors. Cancer Cell 30:940-952
Singal, Amit G; Gupta, Samir; Tiro, Jasmin A et al. (2016) Outreach invitations for FIT and colonoscopy improve colorectal cancer screening rates: A randomized controlled trial in a safety-net health system. Cancer 122:456-63
Knudsen, Erik S; O'Reilly, Eileen M; Brody, Jonathan R et al. (2016) Genetic Diversity of Pancreatic Ductal Adenocarcinoma and Opportunities for Precision Medicine. Gastroenterology 150:48-63
Gallardo-Montejano, Violeta I; Saxena, Geetu; Kusminski, Christine M et al. (2016) Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1α/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat Commun 7:12723
Skinner, Celette Sugg; Gupta, Samir; Halm, Ethan A et al. (2016) Development of the Parkland-UT Southwestern Colonoscopy Reporting System (CoRS) for evidence-based colon cancer surveillance recommendations. J Am Med Inform Assoc 23:402-6
McNamara, Ryan P; Reeder, Jonathan E; McMillan, Elizabeth A et al. (2016) KAP1 Recruitment of the 7SK snRNP Complex to Promoters Enables Transcription Elongation by RNA Polymerase II. Mol Cell 61:39-53
Belfatto, Antonella; White, Derek A; Mason, Ralph P et al. (2016) Tumor radio-sensitivity assessment by means of volume data and magnetic resonance indices measured on prostate tumor bearing rats. Med Phys 43:1275-84
Wylie, Annika; Jones, Amanda E; D'Brot, Alejandro et al. (2016) p53 genes function to restrain mobile elements. Genes Dev 30:64-77
Fernando, W Shirangi; Martins, André F; Zhao, Piyu et al. (2016) Breaking the Barrier to Slow Water Exchange Rates for Optimal Magnetic Resonance Detection of paraCEST Agents. Inorg Chem 55:3007-14
Singal, Amit G; Tiro, Jasmin A; Marrero, Jorge A et al. (2016) Mailed Outreach Program Increases Ultrasound Screening of Patients with Cirrhosis for Hepatocellular Carcinoma. Gastroenterology :

Showing the most recent 10 out of 323 publications