Live Cell Imaging Shared Resource currently houses eleven sophisticated light microscopes and two image analysis workstations that are available to the campus research community for an hourly fee. The instrumentation includes: two laser scanning confocal microscopes, one with two photon capability;one spinning disk laser confocal;two wide field epifluorescence deconvolution microscopes;a Metasystems screening microscope;a fluorescence lifetime microscope for measurement of FRET and two microscopes capable of TIRF. Most of these scopes are equipped with 37?C incubation and four of them also have 5% C02. Five of these microscopes have linear encoded stages for point visiting experiments. The director and staff provide individual training and assistance to users of the facility. Services also include advice on protocol development, troubleshooting of experiments and help with the appropriate interpretation and effective presentation of imaging data. Investigators who wish to use the facility typically meet with the Director to discuss which microscope would be most suitable for their project and to determine appropriate protocols for sample preparation and experimental design. The director serves as consultant or collaborator on projects requiring more than routine microscope imaging. The facility manager is available during normal working hours to assist users. Approved users may check out a key for after hours access. A web-based calendaring system is used to reserve time. It is integrated with a user accounts database and an authentication server for tracking actual usage. The LCIF provides short-term backup of data on a network accessible file server. Since its inception in 2004, the LCIF has trained more than 130 users from 96 laboratories in 26 departments on the UT Southwestern campus, resulting in 63 publications that include data obtained on LCIF instruments. About 30% of LCIF users are Cancer Center investigators. Since 2006, 18 peer-reviewed publications by Cancer Center members include data acquired in the LCIF. Beyond merely providing affordable microscopy, the LCIF actively evaluates emerging technologies and assures their appropriate implementation, as well as promoting scientifically rigorous application of existing technology. This means that both imaging novices and sophisticated users can effectively leverage the facility to avail themselves of the tremendous power of modern light microscopy as a tool for scientific discovery.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA142543-04
Application #
8519962
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
4
Fiscal Year
2013
Total Cost
$68,099
Indirect Cost
$27,467
Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Wong, Mandy S; Wright, Woodring E; Shay, Jerry W (2014) Alternative splicing regulation of telomerase: a new paradigm? Trends Genet 30:430-8
Chau, Vincent; Lim, S Kyun; Mo, Wei et al. (2014) Preclinical therapeutic efficacy of a novel pharmacologic inducer of apoptosis in malignant peripheral nerve sheath tumors. Cancer Res 74:586-97
Iscla, Irene; Wray, Robin; Wei, Shuguang et al. (2014) Streptomycin potency is dependent on MscL channel expression. Nat Commun 5:4891
Fu, Peng; Johnson, Melissa; Chen, Hong et al. (2014) Carpatamides A-C, cytotoxic arylamine derivatives from a marine-derived Streptomyces sp. J Nat Prod 77:1245-8
Park, Jason Y; Gunpat, Joshua; Liu, Li et al. (2014) Red-shifted emission from 1,2-dioxetane-based chemiluminescent reactions. Luminescence 29:553-8
Gerber, David E; Laccetti, Andrew L; Xuan, Lei et al. (2014) Impact of prior cancer on eligibility for lung cancer clinical trials. J Natl Cancer Inst 106:
Yang, Liu; Kwon, Junghee; Popov, Yury et al. (2014) Vascular endothelial growth factor promotes fibrosis resolution and repair in mice. Gastroenterology 146:1339-50.e1
Li, Biaoru; Ding, Lianghao; Yang, Chinrang et al. (2014) Characterization of transcription factor networks involved in umbilical cord blood CD34+ stem cells-derived erythropoiesis. PLoS One 9:e107133
Ludlow, Andrew T; Robin, Jerome D; Sayed, Mohammed et al. (2014) Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution. Nucleic Acids Res 42:e104
King, Kelly A; Gordon-Salant, Sandra; Pawlowski, Karen S et al. (2014) Hearing loss is an early consequence of Npc1 gene deletion in the mouse model of Niemann-Pick disease, type C. J Assoc Res Otolaryngol 15:529-41

Showing the most recent 10 out of 83 publications