In recent years, it has become necessary to take multiple approaches to comprehensively address a question or test a hypothesis in biomedical research. For labs that do not have biochemical expertise, the Biochemical Pharmacology Core offers help that facilitates their research. In the past nine years, the Core has offered short- and long-term assistance to mainly chemists and behavioral scientists in doing receptor binding, immunoblotting and Signaling following receptor activation. The work has resulted in many publications and provided preliminary results for several grant applications.
The specific aims of this application are for the Core to prove the following services: (1) Receptor binding: opioid. dopamine and nicotinic receptors and others when necessary;(2) Receptor autoradiography: opioid receptors and others when required;(3) Assessment of receptor activation by determination of cAMP level. [35S]GTPgS binding, and p44/42 MAP kinase phosphorylation;(4) Protein gel electrophoresis and immunoblotting;(5) Assessment of suitability of receptor antibodies for immunoblotting: opioid receptors initially and others when needed. The services that the Core will provide are valuable to researchers in the drug abuse field, both within and outside of Temple University. The latter group includes Drs. David Y.-W. Lee, Bruce Cohen and Cecile Beguin of Harvard Medical School, Dr. Julie Blendy, University of Pennsylvania, Dr. Michelle Ehrlich of Mt. Sinai School of Medicine. Dr. Sari Izenwasser of University of Miami. Since Dr. Liu-Chen's lab has a great deal of experience in these techniques, the data are of high quality. Dr Liu-Chen's lab has generated and purified MOPR antibodies and has worked out conditions that the antibodies worked well for immunoblotting. A new addition in this renewal is that the Core will perform immunoblotting of endogenous MOPR and/or will provide purified MOPR antibodies for researchers. Moreover, the Core will evaluate antibodies against other molecules of interest in the drug abuse area (opioid receptors and possibly others) for immunoblotting. If so, these antibodies will be valuable tools for researchers in the field. Thus, the Core is a valuable resource for researchers in the drug abuse field whose expertise is outside of biochemical area.

Public Health Relevance

The core provides biochemical pharmacology services to scientists in the drug abuse field whose expertise is non-biochemical in nature. The services will facilitate the progress of many research programs and advance our knowledge of the mechanisms underlying drug abuse and addiction.

National Institute of Health (NIH)
National Institute on Drug Abuse (NIDA)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDA1-EXL-T)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Temple University
United States
Zip Code
Brailoiu, G Cristina; Deliu, Elena; Console-Bram, Linda M et al. (2016) Cocaine inhibits store-operated Ca2+ entry in brain microvascular endothelial cells: critical role for sigma-1 receptors. Biochem J 473:1-5
Nayak, Sunil; Roberts, Adam; Bires, Kristofer et al. (2016) Benzodiazepine inhibits anxiogenic-like response in cocaine or ethanol withdrawn planarians. Behav Pharmacol 27:556-8
Reichenbach, Zachary Wilmer; Li, Hongbo; Ward, Sara Jane et al. (2016) The CB1 antagonist, SR141716A, is protective in permanent photothrombotic cerebral ischemia. Neurosci Lett 630:9-15
Park, Soonhong; Ahuja, Malini; Kim, Min Seuk et al. (2016) Fusion of lysosomes with secretory organelles leads to uncontrolled exocytosis in the lysosomal storage disease mucolipidosis type IV. EMBO Rep 17:266-78
Hudry, E; Martin, C; Gandhi, S et al. (2016) Exosome-associated AAV vector as a robust and convenient neuroscience tool. Gene Ther 23:380-92
Meza-Aviña, Maria Elena; Lingerfelt, Mary A; Console-Bram, Linda M et al. (2016) Design, synthesis, and analysis of antagonists of GPR55: Piperidine-substituted 1,3,4-oxadiazol-2-ones. Bioorg Med Chem Lett 26:1827-30
Cornwell, William D; Wagner, Wendeline; Lewis, Mark G et al. (2016) Effect of chronic morphine administration on circulating dendritic cells in SIV-infected rhesus macaques. J Neuroimmunol 295-296:30-40
Holliday, Erica D; Nucero, Paul; Kutlu, Munir G et al. (2016) Long-term effects of chronic nicotine on emotional and cognitive behaviors and hippocampus cell morphology in mice: comparisons of adult and adolescent nicotine exposure. Eur J Neurosci :
Persidsky, Yuri; Hill, Jeremy; Zhang, Ming et al. (2016) Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab 36:794-807
Gregg, Ryan A; Hicks, Callum; Nayak, Sunil U et al. (2016) Synthetic cathinone MDPV downregulates glutamate transporter subtype I (GLT-1) and produces rewarding and locomotor-activating effects that are reduced by a GLT-1 activator. Neuropharmacology 108:111-9

Showing the most recent 10 out of 287 publications