The overarching goal of the Sampling and Separation Core is to support diverse measurements of signaling peptides and proteins using a range of approaches and then provide these samples to the Protein Identification Core for analysis. The chemical, temporal and spatial diversity of cell-cell signaling molecules requires significant expertise and planning to allow successful peptidomics and proteomics measurements. To profile signaling peptides and proteins in specific brain tissues and cells, this core specializes in: (1) collecting and fractionating peptides and proteins from biological samples, (2) creating directed sampling methodologies for single cells, spatially distinct brain regions, and biological fluids, (3) optimizing sample fractionation approaches including low-volume multidimensional separations, and (4) preparing tissues for mass spectrometry imaging. In cases where protocols and techniques do not exist for to accomplish the measurements goals, the appropriate technologies are developed. This suite of technologies will be applied to profile the signaling peptides and proteins within the nervous system in the study of fundamental neuroscience as well as pathological conditions such as drug-of-abuse-induced changes. Samples range from intact brain regions to single cells, from probing the chemical content of a tissue to measuring the subset that undergoes activity-dependent release, and from acquiring information on spatial to temporal changes in the signaling peptide complement. The core offers the flexibility to individually tailor isolation and separation methodologies for signaling peptides and related proteins inherently unique to the neurobiological phenomena under investigation by each major user.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Center Core Grants (P30)
Project #
5P30DA018310-10
Application #
8471680
Study Section
Special Emphasis Panel (ZDA1-RXL-E)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
10
Fiscal Year
2013
Total Cost
$233,797
Indirect Cost
$82,553
Name
University of Illinois Urbana-Champaign
Department
Type
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820
Lee, Chang Young; Fan, Yi; Rubakhin, Stanislav S et al. (2016) A neuron-in-capillary platform for facile collection and mass spectrometric characterization of a secreted neuropeptide. Sci Rep 6:26940
Hu, Caroline K; Southey, Bruce R; Romanova, Elena V et al. (2016) Identification of prohormones and pituitary neuropeptides in the African cichlid, Astatotilapia burtoni. BMC Genomics 17:660
Durbin, Kenneth R; Fornelli, Luca; Fellers, Ryan T et al. (2016) Quantitation and Identification of Thousands of Human Proteoforms below 30 kDa. J Proteome Res 15:976-82
Southey, Bruce R; Zhu, Ping; Carr-Markell, Morgan K et al. (2016) Characterization of Genomic Variants Associated with Scout and Recruit Behavioral Castes in Honey Bees Using Whole-Genome Sequencing. PLoS One 11:e0146430
Wu, Qian; Comi, Troy J; Li, Bin et al. (2016) On-Tissue Derivatization via Electrospray Deposition for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Endogenous Fatty Acids in Rat Brain Tissues. Anal Chem 88:5988-95
Gonzalez-Pena, Dianelys; Nixon, Scott E; O'Connor, Jason C et al. (2016) Microglia Transcriptome Changes in a Model of Depressive Behavior after Immune Challenge. PLoS One 11:e0150858
Jansson, Erik T; Comi, Troy J; Rubakhin, Stanislav S et al. (2016) Single Cell Peptide Heterogeneity of Rat Islets of Langerhans. ACS Chem Biol 11:2588-95
Savaryn, John Paul; Skinner, Owen S; Fornelli, Luca et al. (2016) Targeted analysis of recombinant NF kappa B (RelA/p65) by denaturing and native top down mass spectrometry. J Proteomics 134:76-84
Ong, Ta-Hsuan; Romanova, Elena V; Roberts-Galbraith, Rachel H et al. (2016) Mass Spectrometry Imaging and Identification of Peptides Associated with Cephalic Ganglia Regeneration in Schmidtea mediterranea. J Biol Chem 291:8109-20
Livnat, Itamar; Tai, Hua-Chia; Jansson, Erik T et al. (2016) A d-Amino Acid-Containing Neuropeptide Discovery Funnel. Anal Chem 88:11868-11876

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