The overarching goal of the Sampling and Separation Core is to support diverse measurements of signaling peptides and proteins using a range of approaches and then provide these samples to the Protein Identification Core for analysis. The chemical, temporal and spatial diversity of cell-cell signaling molecules requires significant expertise and planning to allow successful peptidomics and proteomics measurements. To profile signaling peptides and proteins in specific brain tissues and cells, this core specializes in: (1) collecting and fractionating peptides and proteins from biological samples, (2) creating directed sampling methodologies for single cells, spatially distinct brain regions, and biological fluids, (3) optimizing sample fractionation approaches including low-volume multidimensional separations, and (4) preparing tissues for mass spectrometry imaging. In cases where protocols and techniques do not exist for to accomplish the measurements goals, the appropriate technologies are developed. This suite of technologies will be applied to profile the signaling peptides and proteins within the nervous system in the study of fundamental neuroscience as well as pathological conditions such as drug-of-abuse-induced changes. Samples range from intact brain regions to single cells, from probing the chemical content of a tissue to measuring the subset that undergoes activity-dependent release, and from acquiring information on spatial to temporal changes in the signaling peptide complement. The core offers the flexibility to individually tailor isolation and separation methodologies for signaling peptides and related proteins inherently unique to the neurobiological phenomena under investigation by each major user.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Center Core Grants (P30)
Project #
5P30DA018310-10
Application #
8471680
Study Section
Special Emphasis Panel (ZDA1-RXL-E)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
10
Fiscal Year
2013
Total Cost
$233,797
Indirect Cost
$82,553
Name
University of Illinois Urbana-Champaign
Department
Type
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820
Skinner, Owen S; Catherman, Adam D; Early, Bryan P et al. (2014) Fragmentation of integral membrane proteins in the gas phase. Anal Chem 86:4627-34
Southey, Bruce R; Lee, Ji Eun; Zamdborg, Leonid et al. (2014) Comparing label-free quantitative peptidomics approaches to characterize diurnal variation of peptides in the rat suprachiasmatic nucleus. Anal Chem 86:443-52
Liu, Jing-Xin; Aerts, Jordan T; Rubakhin, Stanislav S et al. (2014) Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry. Analyst 139:5835-42
LeDuc, Richard D; Fellers, Ryan T; Early, Bryan P et al. (2014) The C-score: a Bayesian framework to sharply improve proteoform scoring in high-throughput top down proteomics. J Proteome Res 13:3231-40
Akhtar, Malik N; Southey, Bruce R; Andrén, Per E et al. (2014) Identification of best indicators of peptide-spectrum match using a permutation resampling approach. J Bioinform Comput Biol 12:1440001
Moroz, Leonid L; Kocot, Kevin M; Citarella, Mathew R et al. (2014) The ctenophore genome and the evolutionary origins of neural systems. Nature 510:109-14
Akhtar, Malik N; Southey, Bruce R; Andrén, Per E et al. (2014) Accurate assignment of significance to neuropeptide identifications using Monte Carlo k-permuted decoy databases. PLoS One 9:e111112
Cannon, Joe R; Cammarata, Michael B; Robotham, Scott A et al. (2014) Ultraviolet photodissociation for characterization of whole proteins on a chromatographic time scale. Anal Chem 86:2185-92
Ota, Nobutoshi; Rubakhin, Stanislav S; Sweedler, Jonathan V (2014) D-Alanine in the islets of Langerhans of rat pancreas. Biochem Biophys Res Commun 447:328-33
Li, Yihan; Compton, Philip D; Tran, John C et al. (2014) Optimizing capillary electrophoresis for top-down proteomics of 30-80 kDa proteins. Proteomics 14:1158-64

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