FACILITIES, EQUIPMENT AND OTHER RESOURCES MOLECULAR PROFILING AND CHARACTERIZATION CORE NORTHWESTERN UNIVERSITY The research enterprise at Northwestern University is distinguished by its ability to define and lead interdisciplinary research. From the University culture of cooperation and collaboration, as laid out in the University's strategic plan, interdisciplinary research is identified as one of Northwestern's hallmarks and a foundation upon which Northwestern will build its future. Laboratory: The Kelleher research team develops next-generation proteomics using whole proteins (i.e., the "Top-Down" approach) and efficiently translates this frontier approach to Northwestern. The team uses a diversity of commercial and custom sample handling approaches, mass spectrometers and software to apply high performance and cutting-edge proteomics to a variety of cell lines, tissues and mammalian tissues/fluids. The research group has two wet labs equipped with chemical fume hoods, biosafety cabinets, vacuum lines, gas lines, ice machines, four-80C freezers, five -20C freezers, two refrigerators and a walk-in cold room. Further, the group has all of the instruments necessary for producing nano-chromatographic columns as well as equipment and tools for both basic machining and electronic test and measure.

National Institute of Health (NIH)
National Institute on Drug Abuse (NIDA)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDA1-EXL-T (02))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Illinois Urbana-Champaign
United States
Zip Code
Skinner, Owen S; Catherman, Adam D; Early, Bryan P et al. (2014) Fragmentation of integral membrane proteins in the gas phase. Anal Chem 86:4627-34
Southey, Bruce R; Lee, Ji Eun; Zamdborg, Leonid et al. (2014) Comparing label-free quantitative peptidomics approaches to characterize diurnal variation of peptides in the rat suprachiasmatic nucleus. Anal Chem 86:443-52
Liu, Jing-Xin; Aerts, Jordan T; Rubakhin, Stanislav S et al. (2014) Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry. Analyst 139:5835-42
LeDuc, Richard D; Fellers, Ryan T; Early, Bryan P et al. (2014) The C-score: a Bayesian framework to sharply improve proteoform scoring in high-throughput top down proteomics. J Proteome Res 13:3231-40
Akhtar, Malik N; Southey, Bruce R; Andrén, Per E et al. (2014) Identification of best indicators of peptide-spectrum match using a permutation resampling approach. J Bioinform Comput Biol 12:1440001
Moroz, Leonid L; Kocot, Kevin M; Citarella, Mathew R et al. (2014) The ctenophore genome and the evolutionary origins of neural systems. Nature 510:109-14
Akhtar, Malik N; Southey, Bruce R; Andrén, Per E et al. (2014) Accurate assignment of significance to neuropeptide identifications using Monte Carlo k-permuted decoy databases. PLoS One 9:e111112
Cannon, Joe R; Cammarata, Michael B; Robotham, Scott A et al. (2014) Ultraviolet photodissociation for characterization of whole proteins on a chromatographic time scale. Anal Chem 86:2185-92
Ota, Nobutoshi; Rubakhin, Stanislav S; Sweedler, Jonathan V (2014) D-Alanine in the islets of Langerhans of rat pancreas. Biochem Biophys Res Commun 447:328-33
Li, Yihan; Compton, Philip D; Tran, John C et al. (2014) Optimizing capillary electrophoresis for top-down proteomics of 30-80 kDa proteins. Proteomics 14:1158-64

Showing the most recent 10 out of 132 publications