The Tissue/Cell Culture Core (TC core) produces in vitro models for study of cochlear development, function, and response to deafening or trauma. The goals of the TC core are to (1) assist investigators in producing cultures for use in their research on the cochlea;(2) assist investigators in genetic manipulation of cultured cochlear cells and tissues using transfection, virus-mediated gene transfer, and the use of genetically modified animals;(3) assist investigators in developing and optimizing new in vitro model systems;(4) train investigators, students, postdocs, residents, and staff in culture techniques and troubleshoot problems;(5) coordinate multidisciplinary experiments among investigators and the Genomics and Histology &Imaging cores, which will use live cultures prepared in the TC core;(6) promote and support collaborations among investigators to use multidisciplinary approaches with in vitro models or parallel in vivo and in vitro models. To these ends the TC core will maintain two well-equipped tissue culture facilities in which production of cultures and training can be carried out. The TC core co-Directors Drs. Steven Green and Marian Hansen have almost unequaled experience in rat and mouse cochlear culture systems including dissociated cell cultures from spiral ganglion, cocultures of spiral ganglion neurons and hair cells, and intact organotypic cochlear explant cultures. The co-Directors have experience in gene transfer into cultured cells by transfection, gene gun, adenoviral and lentiviral vectors, and in manipulating gene expression in cultures from transgenic mice with conditional Cre recombinase expression. Thus, the TC core will be able to achieve the stated goals to assist and train investigators and catalyze pilot projects to develop new methods and establish new collaborations. To further promote information sharing and collaborations, the TC core will conduct classes in TC techniques, in addition to informal training, and participate in the semiannual Center symposia at which investigators will present and discuss research results and techniques. Drs. Robin Davis and Stefan Heller, experts in cochlear culture and auditory neuronal stem cells are members of the P30 external advisory committee who will annually review TC core operation and advise the co-Directors.
Tissue and cell culture have been an essential part of neurobiology nearly since the beginnings of systematic experimentation because these methods allow experimental manipulations not possible in vivo. The TC core will exploit our distinctive experience in cochlear cultures to facilitate use of in vitro experimental systems, greatly expanding the types of experiments that can be done and enhancing the network of collaborations.
|Fritzsch, Bernd; Pan, Ning; Jahan, Israt et al. (2015) Inner ear development: building a spiral ganglion and an organ of Corti out of unspecified ectoderm. Cell Tissue Res 361:24-Jul|
|Fritzsch, Bernd; Jahan, Israt; Pan, Ning et al. (2015) Evolving gene regulatory networks into cellular networks guiding adaptive behavior: an outline how single cells could have evolved into a centralized neurosensory system. Cell Tissue Res 359:295-313|
|Tuft, Bradley W; Xu, Linjing; White, Scott P et al. (2014) Neural pathfinding on uni- and multidirectional photopolymerized micropatterns. ACS Appl Mater Interfaces 6:11265-76|
|Ahmad, Iram; Yue, Wei Ying; Fernando, Augusta et al. (2014) p75NTR is highly expressed in vestibular schwannomas and promotes cell survival by activating nuclear transcription factor ?B. Glia 62:1699-712|
|Swiderski, Ruth E; Nakano, Yoko; Mullins, Robert F et al. (2014) A mutation in the mouse ttc26 gene leads to impaired hedgehog signaling. PLoS Genet 10:e1004689|
|Boekhoff-Falk, Grace; Eberl, Daniel F (2014) The Drosophila auditory system. Wiley Interdiscip Rev Dev Biol 3:179-91|
|Bailey, Erin M; Green, Steven H (2014) Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats. J Neurosci 34:13110-26|
|Mao, YanYan; Reiprich, Simone; Wegner, Michael et al. (2014) Targeted deletion of Sox10 by Wnt1-cre defects neuronal migration and projection in the mouse inner ear. PLoS One 9:e94580|
|Christie, Kevin W; Eberl, Daniel F (2014) Noise-induced hearing loss: new animal models. Curr Opin Otolaryngol Head Neck Surg 22:374-83|
|Inagaki, Akira; Frank, C Andrew; Usachev, Yuriy M et al. (2014) Pharmacological correction of gating defects in the voltage-gated Ca(v)2.1 Ca²? channel due to a familial hemiplegic migraine mutation. Neuron 81:91-102|
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