The Stanford OHNS Core Center is organized into two Core facilities: Core I - Imaging Core and Core II - Inner Ear Physiology Core. Both cores serve to facilitate interdisciplinary collaborative research on the basic mechanisms of inner ear function, development, and therapeutic strategies. The Core Center user base comprises more than a dozen Principal Investigators working on a broad range of molecular, biophysical, developmental, whole animal, as well as engineering based projects all on topics that are of relevance to the mission of the NIDCD. Three goals are being pursued in both cores with an overarching strategy and philosophy where the Core Center serves as a hub for knowledge and technology (Aim 1) with the goal of generating an informed user base where students, postdoctoral fellows, and faculty share successes but most importantly also problems and solutions. This, in turn, stimulates collaborative research (Aim 2) because it teaches critical thinking and solving problems as a team. Finally, the Core Center makes available access to state-of-the-art technology (Aim 3) uniquely applicable to solving inner ear-related questions and research. These resources would be out of reach for a single laboratory simply because the costs to maintain and appropriately utilize the equipment would be economically unbearable.

Public Health Relevance

The Core Center facilitates collaborative research for 10 qualifying RO1 grants and serves as hub for sharing knowledge and technology among a group of researchers working on topics of relevance for the NIDCD. Focus lies on providing access to state-of-the-art technology to a vibrant collaborative group of users to foster collaborations and to increase the overall quality of research on topics of relevance for the NIDCD. Core I Imaging Core Co-Directors: Anthony J. Ricci, Ph.D. John S. Oghalai, M.D. DESCRIPTION (provided by applicant): The Imaging Core provides access to knowledge and training allowing the user to learn and understand imaging technologies and sample preparation techniques that are tailored toward inner ear-related research. Beside standard equipment for sample preparation and microscopy, it also offers advanced imaging systems that are integrated into a physiology-oriented concept allowing access to a broad base of users of technology that is not available as off the shelf solution. The Imaging Core is integrated into the overall philosophy of the Stanford OHNS Core Center providing access to knowledge and technology (Aim 1), and to stimulate and to inspire discussion among users to identify the best possible imaging solution for a specific question. Previous success supports the strategy that advanced scientific discussions foster and stimulate collaborative research (Aim 2). The Core provides access and training for state-of-the-art confocal systems (Aim 3), each configured for specific tasks, such as routine confocal scanning in upright or inverted configurations, fast high resolution scanning, ultrafast scanning for imaging physiological processes in subcellular structures or spinning disk confocal imaging for living cells. In addition, users have access to a highly flexible 2-photon imaging system that allows imaging of isolated tissues and structures in living animals. For histology, the Core offers access and training to use apparatus for cryosectioning as well as paraffin embedding and sectioning. PUBLIC HEALTH RELEVANCE: Access to equipment that requires expert maintenance and advanced training for optimal use of the individual instruments'capabilities can most efficiently be capitalized through the implemented Core Center. For an individual laboratory, it would be economically unbearable to maintain such broad and up-to-date capabilities. Implementing a philosophy where the user base is being educated to utilize the Core-provided technology in the best possible way creates a stimulating and innovative environment for collaborative research.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
2P30DC010363-06
Application #
8669452
Study Section
Special Emphasis Panel (ZDC1)
Program Officer
Platt, Christopher
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Stanford University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
City
Stanford
State
CA
Country
United States
Zip Code
94304
Ealy, Megan; Ellwanger, Daniel C; Kosaric, Nina et al. (2016) Single-cell analysis delineates a trajectory toward the human early otic lineage. Proc Natl Acad Sci U S A 113:8508-13
Lee, Hee Yoon; Raphael, Patrick D; Xia, Anping et al. (2016) Two-Dimensional Cochlear Micromechanics Measured In Vivo Demonstrate Radial Tuning within the Mouse Organ of Corti. J Neurosci 36:8160-73
Zeng, Wei-Zheng; Grillet, Nicolas; Dewey, James B et al. (2016) Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function. J Neurosci 36:9201-16
Sundaresan, Srividya; Kong, Jee-Hyun; Fang, Qing et al. (2016) Thyroid hormone is required for pruning, functioning and long-term maintenance of afferent inner hair cell synapses. Eur J Neurosci 43:148-61
Sundaresan, S; Balasubbu, S; Mustapha, M (2016) Thyroid hormone is required for the pruning of afferent type II spiral ganglion neurons in the mouse cochlea. Neuroscience 312:165-78
Xia, Anping; Liu, Xiaofang; Raphael, Patrick D et al. (2016) Hair cell force generation does not amplify or tune vibrations within the chicken basilar papilla. Nat Commun 7:13133
Kim, Sangmin; Raphael, Patrick D; Oghalai, John S et al. (2016) High-speed spectral calibration by complex FIR filter in phase-sensitive optical coherence tomography. Biomed Opt Express 7:1430-44
Durruthy-Durruthy, Robert; Heller, Stefan (2015) Applications for single cell trajectory analysis in inner ear development and regeneration. Cell Tissue Res 361:49-57
Huth, Markus E; Han, Kyu-Hee; Sotoudeh, Kayvon et al. (2015) Designer aminoglycosides prevent cochlear hair cell loss and hearing loss. J Clin Invest 125:583-92
Lee, Hee Yoon; Raphael, Patrick D; Park, Jesung et al. (2015) Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea. Proc Natl Acad Sci U S A 112:3128-33

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