The Transgenic and ES Cell Core of the Washington University Diabetes Research Center (DRC) is a shared facility whose goal is the efficient and cost-effective development of genetically-altered mouse models to elucidate the pathogenesis of diabetes and its complications and explore related areas of endocrinology and metabolism. These animal models provide an important avenue for pre-clinical studies that will serve as the basis for translating basic research advances into clinical practice. Importantly, in vivo models allow the analysis of gene function in the context of complex intercellular and integrative systemic responses needed for the study of diabetes, a polygenic disorder involving multiple tissue types and environmental influences. Current molecular genetic techniques allow spatially and temporally restricted analysis of gene effects (both inactivation and over-expression) in mice using altered systems alone or in combination, leading to previously unprecedented creativity and resolution in analysis. Although the concepts involved in generating transgenic (Tg) or knockout/knockin (KO/KI) animals are in general straightforward, the equipment, facilities, and personnel with the appropriate technical skills are either impractical or too expensive for most labs to implement independently. With continued enthusiastic demand for assistance in the generation of genetically altered mice, the DRC Transgenic and ES Cell Core will continue to provide the necessary expertise, facilities, equipment, and personnel to efficiently generate Tg and KO/KI mice for DRC members and assist in propagating pooriy breeding animals. Additional services include marker assisted generation of congenic lines and transient Cre of FLP recombinase expression in zygotes by microinjection of expression plasmids. To accomplish this, the Core will utilize pre-existing, in-house, highly successful facilities - the Washington University Transgenic Vectors Core, Siteman Cancer Center ES Cell Core, and Mouse Genetics Core. Utilization of these facilities for vector design/construction, electroporation, and DNA/ES cell microinjection precludes the need for the Transgenic and ES Cell Core to purchase and set up expensive equipment and independently hire/train/manage our own personnel.

Public Health Relevance

Diabetes is an increasing health problem in the US and around the worid. Generation and analysis of small animal models to studies diabetes and its complications has provided a better understanding of the disease in humans and allowed for the testing of potential therapies. Research facilitated by the services of the DRC Transgenic and ES Cell Core will have a great impact on understanding of human diabetes.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-S)
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Washington University
Saint Louis
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Gaut, Joseph P; Crimmins, Dan L; Ohlendorf, Matt F et al. (2014) Development of an immunoassay for the kidney-specific protein myo-inositol oxygenase, a potential biomarker of acute kidney injury. Clin Chem 60:747-57
Hsu, Fong-Fu; Kuhlmann, F Matthew; Turk, John et al. (2014) Multiple-stage linear ion-trap with high resolution mass spectrometry towards complete structural characterization of phosphatidylethanolamines containing cyclopropane fatty acyl chain in Leishmania infantum. J Mass Spectrom 49:201-9
Calderon, Boris; Carrero, Javier A; Unanue, Emil R (2014) The central role of antigen presentation in islets of Langerhans in autoimmune diabetes. Curr Opin Immunol 26:32-40
Vigueira, Patrick A; McCommis, Kyle S; Schweitzer, George G et al. (2014) Mitochondrial pyruvate carrier 2 hypomorphism in mice leads to defects in glucose-stimulated insulin secretion. Cell Rep 7:2042-53
Fabbrini, Elisa; Serafini, Mauro; Colic Baric, Irena et al. (2014) Effect of plasma uric acid on antioxidant capacity, oxidative stress, and insulin sensitivity in obese subjects. Diabetes 63:976-81
Asombang, Akwi W; Rahman, Rubayat; Ibdah, Jamal A (2014) Gastric cancer in Africa: current management and outcomes. World J Gastroenterol 20:3875-9
Yoshino, Jun; Almeda-Valdes, Paloma; Patterson, Bruce W et al. (2014) Diurnal variation in insulin sensitivity of glucose metabolism is associated with diurnal variations in whole-body and cellular fatty acid metabolism in metabolically normal women. J Clin Endocrinol Metab 99:E1666-70
Schugar, Rebecca C; Moll, Ashley R; André d'Avignon, D et al. (2014) Cardiomyocyte-specific deficiency of ketone body metabolism promotes accelerated pathological remodeling. Mol Metab 3:754-69
Aguirre, Lina; Napoli, Nicola; Waters, Debra et al. (2014) Increasing adiposity is associated with higher adipokine levels and lower bone mineral density in obese older adults. J Clin Endocrinol Metab 99:3290-7
Napoli, Nicola; Shah, Krupa; Waters, Debra L et al. (2014) Effect of weight loss, exercise, or both on cognition and quality of life in obese older adults. Am J Clin Nutr 100:189-98

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