The Hormone Assay and Analytical Services Core (HAASC) provides assistance to investigators in the measurement of hormones, amino acids ( concentration and specific activity), glucose enrichment, lipids, nucleotides, and markers of oxidative stress in biologic fluids and tissue samples. The core provides space, equipment and personnel that performs sample analysis and method development. Investigators pay a fee for service that covers the cost of regents, supplies, a percentage of personnel salary and pro-rated service contracts. Over the last grant cycle this core has dramatically evolved. With support from the institution the core has purchased equipment that will lower overall cost and decrease turnaround time for our standard high throughput assays and are continuing to offer cost effective new hormone assays to our investigators. The core has developed NMR assays to assess the enrichment of glucose (C2/C5), which is a marker of gluconeogenesis. We have expanded the scope of our services as the needs of VDRTC members change. The core assayed over 30,000 samples in the past year for 32 Vanderbilt investigators and 7 non-Vanderbilt investigators. Over the past grant cycle it provided data to support over 150 publications. The HAASC is jointly supported by the VDRTC and the NIDDK-funded Mouse Metabolic Phenotyping Center. This cooperative arrangement allows the core to offer a wide range of services in a non-overlapping, cost efficient manner. The core is part of the Vanderbilt Core Ordering &Reporting Enterprise Systemtm, which provides an efficient billing system and oversight and governance for the core. The Hormone Assay and Analytical Services Core, in operation for more than 30 years, continues to provide essential services that support the research of DRTC-affiliated investigators in the next funding cycle.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020593-35
Application #
8469486
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
35
Fiscal Year
2013
Total Cost
$211,509
Indirect Cost
$75,927
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Moore, Mary Courtney; Smith, Marta S; Farmer, Ben et al. (2018) Morning Hyperinsulinemia Primes the Liver for Glucose Uptake and Glycogen Storage Later in the Day. Diabetes 67:1237-1245
Cooke, Allison L; Morris, Jamie; Melchior, John T et al. (2018) A thumbwheel mechanism for APOA1 activation of LCAT activity in HDL. J Lipid Res 59:1244-1255
Moore, Mary Courtney; Kelley, David E; Camacho, Raul C et al. (2018) Superior Glycemic Control With a Glucose-Responsive Insulin Analog: Hepatic and Nonhepatic Impacts. Diabetes 67:1173-1181
Funkhouser-Jones, Lisa J; van Opstal, Edward J; Sharma, Ananya et al. (2018) The Maternal Effect Gene Wds Controls Wolbachia Titer in Nasonia. Curr Biol 28:1692-1702.e6
Hart, Nathaniel J; Aramandla, Radhika; Poffenberger, Gregory et al. (2018) Cystic fibrosis-related diabetes is caused by islet loss and inflammation. JCI Insight 3:
Uddin, Md Imam; Jayagopal, Ashwath; Wong, Alexis et al. (2018) Real-time imaging of VCAM-1 mRNA in TNF-? activated retinal microvascular endothelial cells using antisense hairpin-DNA functionalized gold nanoparticles. Nanomedicine 14:63-71
Sui, Lina; Danzl, Nichole; Campbell, Sean R et al. (2018) ?-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells. Diabetes 67:26-35
Beach, Lauren B; Elasy, Tom A; Gonzales, Gilbert (2018) Prevalence of Self-Reported Diabetes by Sexual Orientation: Results from the 2014 Behavioral Risk Factor Surveillance System. LGBT Health 5:121-130
Dutter, Brendan F; Ender, Anna; Sulikowski, Gary A et al. (2018) Rhodol-based thallium sensors for cellular imaging of potassium channel activity. Org Biomol Chem 16:5575-5579
Herrick, Mary K; Favela, Kristin M; Simerly, Richard B et al. (2018) Attenuation of diet-induced hypothalamic inflammation following bariatric surgery in female mice. Mol Med 24:56

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