The Metabolic Physiology Shared Resource (MPSR) provides investigators with a platform for the design, execution, and interpretation of highly specialized procedures for conducting experiments in vivo. The MPSR facilitates research for investigators in the range of species spanning from mouse to humans. Many of the basic tenets of experimental design are species-Independent permitting resources and expertise to be pooled under the common MPSR umbrella. This philosophy alleviates experimental constraints by providing access to a variety of model systems and provides for seamless translation of basic experimental findings to humans. The impact and benefits of the MPSR are greater than the sum of Its constituent parts. The MPSR uses a defined mechanism for the design and optimization of experimental protocols using an established Studio format. The Studio (i) brings together Vanderbilt scientists with specific expertise to review a proposal;(ii) identifies potential limitations on the front-end;and (iii) leads to the most efficient use of resources including animals and human volunteers. The MPSR makes complex In vivo experiments feasible by providing specialized animal surgical (e.g. catheter placement, bariatric surgery) and experimental (e.g. clamps, energy balance) services. Advancements in the present cycle have led to the development of bariatric surgery procedures and expansion of resources for energy balance measurements In animals that parallel procedures used In human studies conducted by the MPSR. The MPSR has also added a human clamp component. The addition of these vital services fills out the scope of services leading to comprehensive analyses of insulin action and energy balance from rodents to large animals to human subjects. MPSR services will channel into analytical, statistical, and bioinformatics services, thereby enhancing the utility of this resource.

Public Health Relevance

The MPSR gives DRTC investigators studying obesity and diabetes access to novel in vivo animal model systems which can be extended to human subjects. Conversely findings in patient populations can be studied in the MPSR at a more basic level in animals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020593-36
Application #
8636430
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
36
Fiscal Year
2014
Total Cost
$197,518
Indirect Cost
$70,904
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
King-Morris, Kelli R; Deger, Serpil Muge; Hung, Adriana M et al. (2016) Measurement and Correlation of Indices of Insulin Resistance in Patients on Peritoneal Dialysis. Perit Dial Int 36:433-41
Mani, Bharath K; Osborne-Lawrence, Sherri; Vijayaraghavan, Prasanna et al. (2016) β1-Adrenergic receptor deficiency in ghrelin-expressing cells causes hypoglycemia in susceptible individuals. J Clin Invest 126:3467-78
Gamboa, Jorge L; Billings 4th, Frederic T; Bojanowski, Matthew T et al. (2016) Mitochondrial dysfunction and oxidative stress in patients with chronic kidney disease. Physiol Rep 4:
(2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12:1-222
Crowder, Spencer W; Balikov, Daniel A; Boire, Timothy C et al. (2016) Copolymer-Mediated Cell Aggregation Promotes a Proangiogenic Stem Cell Phenotype In Vitro and In Vivo. Adv Healthc Mater 5:2866-2871
Beavers, Kelsey R; Werfel, Thomas A; Shen, Tianwei et al. (2016) Porous Silicon and Polymer Nanocomposites for Delivery of Peptide Nucleic Acids as Anti-MicroRNA Therapies. Adv Mater 28:7984-7992
Conrad, Elizabeth; Dai, Chunhua; Spaeth, Jason et al. (2016) The MAFB transcription factor impacts islet α-cell function in rodents and represents a unique signature of primate islet β-cells. Am J Physiol Endocrinol Metab 310:E91-E102
Shaffer, Carrie L; Good, James A D; Kumar, Santosh et al. (2016) Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial Pathogens. MBio 7:e00221-16
Delong, Thomas; Wiles, Timothy A; Baker, Rocky L et al. (2016) Pathogenic CD4 T cells in type 1 diabetes recognize epitopes formed by peptide fusion. Science 351:711-4
Leamy, Alexandra K; Hasenour, Clinton M; Egnatchik, Robert A et al. (2016) Knockdown of triglyceride synthesis does not enhance palmitate lipotoxicity or prevent oleate-mediated rescue in rat hepatocytes. Biochim Biophys Acta 1861:1005-14

Showing the most recent 10 out of 536 publications