The purpose of the Joslin Animal Physiology Core is to provide technically advanced physiological evaluation of rodents for the study of diabetes, obesity, and their associated complications. The study of animal models is an integral part of Joslin Research, and DRC invesfigators maintain more than 10,000 mice and rats in the Joslin Animal Facility, and more than 7,000 mice offsite. Joslin DRC invesfigators have generated approximately 150 strains of genefically engineered mice during the last 15 years, the majority of which have been studied using the Animal Physiology Core. To better serve Joslin DRC investigators and enable outside users, a new facility for the Animal Physiology Core Laboratory will be built in 2012 within the Joslin. This new facility will consolidate services in one facility and expand services provided by the Core. Core services provided by the Animal Physiology Core include: Comprehensive Laboratory Monitoring Systems (CLAMS) for the measurement of in vivo energy expenditure, activity and fuel utilization;Dual Energy X-ray Absorptiometry (DEXA) for assessment of body composifion;blood pressure measurement by tail vein and telemetry;systemic blood perfusion studies using Laser Doppler Perfusion Imaging system (LDPI);and hypoxia and hyperoxia using an OxyCycler. New services that are proposed for the Animal Physiology Core include: IVIS Spectrum CT for in vivo animal imaging;diurnal incubator for studies of cold exposure;and rodent treadmill and wheel cages for studies of exercise. The Animal Physiology Core has consistently provided service to DRC investigators (approximately 14/year) and has been essenfial in the publicafion of multiple papers in high impact journals such as Cell Metabolism, the Journal of Clinical Investigation and Nature Medicine. Joslin support for the Animal Physiology Core has been substantial, with total cost for new and replacement instruments of $400,000 in the last three years. In addifion, Joslin has committed to build a new Animal Physiology Core facility and to purchase new equipment that will continue to keep Joslin animal-based research at the forefront of diabetes research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK036836-28
Application #
8725123
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
28
Fiscal Year
2014
Total Cost
$142,571
Indirect Cost
$62,951
Name
Joslin Diabetes Center
Department
Type
DUNS #
071723084
City
Boston
State
MA
Country
United States
Zip Code
02215
Karatepe, Kutay; Zhu, Haiyan; Zhang, Xiaoyu et al. (2018) Proteinase 3 Limits the Number of Hematopoietic Stem and Progenitor Cells in Murine Bone Marrow. Stem Cell Reports 11:1092-1105
Sustarsic, Elahu G; Ma, Tao; Lynes, Matthew D et al. (2018) Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis. Cell Metab 28:159-174.e11
Lessard, Sarah J; MacDonald, Tara L; Pathak, Prerana et al. (2018) JNK regulates muscle remodeling via myostatin/SMAD inhibition. Nat Commun 9:3030
Cardamone, Maria Dafne; Tanasa, Bogdan; Cederquist, Carly T et al. (2018) Mitochondrial Retrograde Signaling in Mammals Is Mediated by the Transcriptional Cofactor GPS2 via Direct Mitochondria-to-Nucleus Translocation. Mol Cell 69:757-772.e7
Solheim, Marie H; Winnay, Jonathon N; Batista, Thiago M et al. (2018) Mice Carrying a Dominant-Negative Human PI3K Mutation Are Protected From Obesity and Hepatic Steatosis but Not Diabetes. Diabetes 67:1297-1309
Stanford, Kristin I; Lynes, Matthew D; Takahashi, Hirokazu et al. (2018) 12,13-diHOME: An Exercise-Induced Lipokine that Increases Skeletal Muscle Fatty Acid Uptake. Cell Metab 27:1111-1120.e3
McGill, Dayna E; Volkening, Lisa K; Butler, Deborah A et al. (2018) Baseline Psychosocial Characteristics Predict Frequency of Continuous Glucose Monitoring in Youth with Type 1 Diabetes. Diabetes Technol Ther 20:434-439
Weir, Gordon C; Ehlers, Mario R; Harris, Kristina M et al. (2018) Alpha-1 antitrypsin treatment of new-onset type 1 diabetes: An open-label, phase I clinical trial (RETAIN) to assess safety and pharmacokinetics. Pediatr Diabetes 19:945-954
Qi, Weier; Li, Qian; Gordin, Daniel et al. (2018) Preservation of renal function in chronic diabetes by enhancing glomerular glucose metabolism. J Mol Med (Berl) 96:373-381
Adachi, Yusuke; De Sousa-Coelho, Ana Luisa; Harata, Ikue et al. (2018) l-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. Mol Metab 17:61-70

Showing the most recent 10 out of 1120 publications