Mass spectrometry techniques, particularly those requiring the measurement of stable (non-radioactive) nuclide enrichment in biological samples, have become established tools in the study of human nutrition and metabolism. Such methods, though technically demanding, are uniquely suited to quantitative In vivo studies under various pathophysiologic states. The Core's initiative to combine whole body tracer approaches with tissue specific mobile PET imaging is a novel means to evaluate metabolic alterations and has significant applicability to obese and non-obese subjects. Likewise, the addition of a metabolomics and lipidomics component to the Core will further enhance the scope of nutritional and metabolic studies available to the NORC-H investigators. This Core, its Director and Co-Directors, as well as its senior technician will provide: 1. Assistance in the design of successful nutritional stable isotope, and PET experiments including tracer selection, isotope preparation for infusion, and consultation regarding institutional review board approval. 2. A metabolomics platform capable of biomarker and metabolite discovery, biomarker and metabolite identification and metabolite quantitation. 3. Efficient and equitable access to Core analytic instruments, accurate analyses of samples, and quality control. 4. Assistance in the development of new methods for analyses of compounds in biologic samples including blood, urine, tissues and expired air. 5. An opportunity for new investigators to learn about the application of stable isotope-tracer techniques, PET and metabolomics in metabolic/nutritional research.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30DK040561-18
Application #
8715765
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Oliveira, Adriana L; Azevedo, Debora C; Bredella, Miriam A et al. (2015) Visceral and subcutaneous adipose tissue FDG uptake by PET/CT in metabolically healthy obese subjects. Obesity (Silver Spring) 23:286-9
Houghteling, Pearl D; Walker, W Allan (2015) Why is initial bacterial colonization of the intestine important to infants' and children's health? J Pediatr Gastroenterol Nutr 60:294-307
Shanmugam, Nanda Kumar N; Trebicka, Estela; Fu, Ling-Lin et al. (2014) Intestinal inflammation modulates expression of the iron-regulating hormone hepcidin depending on erythropoietic activity and the commensal microbiota. J Immunol 193:1398-407
Makimura, Hideo; Murphy, Caitlin A; Feldpausch, Meghan N et al. (2014) The effects of tesamorelin on phosphocreatine recovery in obese subjects with reduced GH. J Clin Endocrinol Metab 99:338-43
Duro, Debora; Mitchell, Paul D; Mehta, Nilesh M et al. (2014) Variability of resting energy expenditure in infants and young children with intestinal failure-associated liver disease. J Pediatr Gastroenterol Nutr 58:637-41
Kraus, Daniel; Yang, Qin; Kong, Dong et al. (2014) Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature 508:258-62
Gathungu, Rose M; Bird, Susan S; Sheldon, Diane P et al. (2014) Identification of metabolites from liquid chromatography-coulometric array detection profiling: gas chromatography-mass spectrometry and refractionation provide essential information orthogonal to LC-MS/microNMR. Anal Biochem 454:23-32
Weng, Meiqian; Ganguli, Kriston; Zhu, Weishu et al. (2014) Conditioned medium from Bifidobacteria infantis protects against Cronobacter sakazakii-induced intestinal inflammation in newborn mice. Am J Physiol Gastrointest Liver Physiol 306:G779-87
Sanderson, Ian R (2014) Growth problems in children with IBD. Nat Rev Gastroenterol Hepatol 11:601-10
Su, Libo; Su, Chien-wen; Qi, Yujuan et al. (2014) Coinfection with an intestinal helminth impairs host innate immunity against Salmonella enterica serovar Typhimurium and exacerbates intestinal inflammation in mice. Infect Immun 82:3855-66

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