The Tissue and Cell Analysis (TCA) Core of The University of Chicago DDRCC provides services vital to the study of intestinal disease, particularly immune-mediated, infectious, neoplastic and inflammatory bowel diseases, in human and animal tissues. In simplest terms, the core components can be broken into seven major areas: (1) anatomic pathology review and consultation;(2) processing of formalin-fixed paraffinembedded mouse tissues;(3) access to human tissues and tissue arrays from the paraffin-embedded tissue archives of the University of Chicago Department of Pathology;(4) high quality banking of snap-frozen tissues from surgical specimens;(5) training and use of laser capture micro-dissection microscopes and cryostats;(6) training and use of the ACIS II automated cellular imaging system;and (7) training and use of 2 photon, confocal, and widefield fluorescence microscopes as well as brightfield photomicroscopes. The TCA Core was created through a 2007 reorganization of the Molecular and Experimental Pathology (MEP) Core in an effort to better meet the needs of the DDRCC membership by integrating tissue processing, imaging, and analysis. This was stimulated by a survey of DDRCC membership, evaluation of core service use, and recognition that several key recommendations made by the reviewers in the 2005 review of this core (as part of the DDRCC renewal) were precisely correct. As was true of the previous MEP Core, the TCA Core has partnered with existing resources to generate significant cost-savings to both the core and the DDRCC membership. The Administrative Director of the Molecular Experimental Pathology Core, Dr. Jerrold Turner M.D., Ph.D., oversees the operations of all components and is directly involved in all human tissue-related operations, provides anatomic pathology review and consultation services, and supervises human and animal tissue immunostaining and tissue array production. The Co-Director of the TCA Core, Dr. Vytautas Bindokas, Ph.D., directs fluorescence microscopy facilities and provides direct assistance and education in image acquisition as well as off-line analysis.

Public Health Relevance

Analysis of human and animal tissues, as well as cultured cells, is required for advanced studies of intestinal disease. The Tissue and Cell Analysis Core provides state-of-the-art imaging resources, a rich library of archival human clinical specimens, essential standard services, and expert consultation to support DDRCC investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK042086-24
Application #
8575334
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
24
Fiscal Year
2014
Total Cost
$101,300
Indirect Cost
$36,364
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
An, Gary; Kulkarni, Swati (2015) An agent-based modeling framework linking inflammation and cancer using evolutionary principles: description of a generative hierarchy for the hallmarks of cancer and developing a bridge between mechanism and epidemiological data. Math Biosci 260:16-24
Wu, Shaoping; Zhang, Yong-Guo; Lu, Rong et al. (2015) Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis. Gut 64:1082-94
Jin, H R; Liao, Y; Li, X et al. (2014) Anticancer compound Oplopantriol A kills cancer cells through inducing ER stress and BH3 proteins Bim and Noxa. Cell Death Dis 5:e1190
Lu, R; Wu, S; Zhang, Y-G et al. (2014) Enteric bacterial protein AvrA promotes colonic tumorigenesis and activates colonic beta-catenin signaling pathway. Oncogenesis 3:e105
McDonald, Benjamin D; Bunker, Jeffrey J; Ishizuka, Isabel E et al. (2014) Elevated T cell receptor signaling identifies a thymic precursor to the TCR??(+)CD4(-)CD8?(-) intraepithelial lymphocyte lineage. Immunity 41:219-29
Li, Yan Chun (2014) Discovery of vitamin D hormone as a negative regulator of the renin-angiotensin system. Clin Chem 60:561-2
Chuang, Alice Y; Chuang, Jim C; Zhai, Zili et al. (2014) NOD2 expression is regulated by microRNAs in colonic epithelial HCT116 cells. Inflamm Bowel Dis 20:126-35
Leone, Vanessa A; Cham, Candace M; Chang, Eugene B (2014) Diet, gut microbes, and genetics in immune function: can we leverage our current knowledge to achieve better outcomes in inflammatory bowel diseases? Curr Opin Immunol 31:16-23
Evans, Christian C; LePard, Kathy J; Kwak, Jeff W et al. (2014) Exercise prevents weight gain and alters the gut microbiota in a mouse model of high fat diet-induced obesity. PLoS One 9:e92193
Jabri, Bana; Chen, Xi; Sollid, Ludvig M (2014) How T cells taste gluten in celiac disease. Nat Struct Mol Biol 21:429-31

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