The Tissue and Cell Analysis (TCA) Core of The University of Chicago DDRCC provides services vital to the study of intestinal disease, particularly immune-mediated, infectious, neoplastic and inflammatory bowel diseases, in human and animal tissues. In simplest terms, the core components can be broken into seven major areas: (1) anatomic pathology review and consultation;(2) processing of formalin-fixed paraffinembedded mouse tissues;(3) access to human tissues and tissue arrays from the paraffin-embedded tissue archives of the University of Chicago Department of Pathology;(4) high quality banking of snap-frozen tissues from surgical specimens;(5) training and use of laser capture micro-dissection microscopes and cryostats;(6) training and use of the ACIS II automated cellular imaging system;and (7) training and use of 2 photon, confocal, and widefield fluorescence microscopes as well as brightfield photomicroscopes. The TCA Core was created through a 2007 reorganization of the Molecular and Experimental Pathology (MEP) Core in an effort to better meet the needs of the DDRCC membership by integrating tissue processing, imaging, and analysis. This was stimulated by a survey of DDRCC membership, evaluation of core service use, and recognition that several key recommendations made by the reviewers in the 2005 review of this core (as part of the DDRCC renewal) were precisely correct. As was true of the previous MEP Core, the TCA Core has partnered with existing resources to generate significant cost-savings to both the core and the DDRCC membership. The Administrative Director of the Molecular Experimental Pathology Core, Dr. Jerrold Turner M.D., Ph.D., oversees the operations of all components and is directly involved in all human tissue-related operations, provides anatomic pathology review and consultation services, and supervises human and animal tissue immunostaining and tissue array production. The Co-Director of the TCA Core, Dr. Vytautas Bindokas, Ph.D., directs fluorescence microscopy facilities and provides direct assistance and education in image acquisition as well as off-line analysis.

Public Health Relevance

Analysis of human and animal tissues, as well as cultured cells, is required for advanced studies of intestinal disease. The Tissue and Cell Analysis Core provides state-of-the-art imaging resources, a rich library of archival human clinical specimens, essential standard services, and expert consultation to support DDRCC investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK042086-24
Application #
8575334
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
24
Fiscal Year
2014
Total Cost
$101,300
Indirect Cost
$36,364
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Lu, Lei; Claud, Erika C (2018) Intrauterine Inflammation, Epigenetics, and Microbiome Influences on Preterm Infant Health. Curr Pathobiol Rep 6:15-21
Lu, Jing; Synowiec, Sylvia; Lu, Lei et al. (2018) Microbiota influence the development of the brain and behaviors in C57BL/6J mice. PLoS One 13:e0201829
Shiloh, Ruth; Gilad, Yuval; Ber, Yaara et al. (2018) Non-canonical activation of DAPK2 by AMPK constitutes a new pathway linking metabolic stress to autophagy. Nat Commun 9:1759
Wang, Haitao; Cheng, Minying; Dsouza, Melissa et al. (2018) Soil Bacterial Diversity Is Associated with Human Population Density in Urban Greenspaces. Environ Sci Technol 52:5115-5124
Khambu, Bilon; Huda, Nazmul; Chen, Xiaoyun et al. (2018) HMGB1 promotes ductular reaction and tumorigenesis in autophagy-deficient livers. J Clin Invest 128:2419-2435
Cason, Cori A; Dolan, Kyle T; Sharma, Gaurav et al. (2018) Plasma microbiome-modulated indole- and phenyl-derived metabolites associate with advanced atherosclerosis and postoperative outcomes. J Vasc Surg 68:1552-1562.e7
Christensen, B; Micic, D; Gibson, P R et al. (2018) Vedolizumab in patients with concurrent primary sclerosing cholangitis and inflammatory bowel disease does not improve liver biochemistry but is safe and effective for the bowel disease. Aliment Pharmacol Ther 47:753-762
Peñalver Bernabé, Beatriz; Cralle, Lauren; Gilbert, Jack A (2018) Systems biology of the human microbiome. Curr Opin Biotechnol 51:146-153
Amin, Ruhul; Asplin, John; Jung, Daniel et al. (2018) Reduced active transcellular intestinal oxalate secretion contributes to the pathogenesis of obesity-associated hyperoxaluria. Kidney Int 93:1098-1107
Lu, Jing; Lu, Lei; Yu, Yueyue et al. (2018) Effects of Intestinal Microbiota on Brain Development in Humanized Gnotobiotic Mice. Sci Rep 8:5443

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