The primary objective of the Physiology Core is to provide DRC members with access to centralized facilities, services and technical expertise to address complex metabolic questions related to diabetes using normal, diabetic or genetically modified rodent models (including rats, and in some cases mice). This fee-for service Core consists of two Sub-cores, the Animal Surgery and Experimental Procedure Sub-core and the Analytical Sub-core, each of which contains specialized equipment and key personnel to help DRC investigators and/or their trainees achieve their tasks in the most efficient and cost-effective manner. It also serves as a forum for collaboration between members with different research backgrounds but a common interest in studying diabetes. Through the Animal Surgery and Experimental Procedure Sub-core, DRC investigators can access training courses, equipment, laboratory facilities and technical expertise to perform surgeries for stereotaxis and the placement of vascular catheters and other implantables, as well as carry out complex metabolic studies using specialized experimental methodologies (e.g. glucose clamps, tracers, microdialysis and amperometric studies) in conscious rodents - skills that are not easily accessible to investigators without previous training or experience. The Analytical Sub-core provides DRC members with a central facility for the measurement of glucoregulatory hormones, cytokines and neurotransmitters derived from the animal studies. This component of the Physiology Core benefits from the expertise and equipment of an on-going and prolific radioimmunoassay and HPLC facility which has recently incorporated Luminex technology and tandem mass spectrometry for measuring cytokines and neurotransmitters, respectively. In addition, DRC investigators can now profile a focused panel of genes using PCR array technology through this sub-core. Together, these two sub-cores provide DRC members with the unique opportunity to systematically address pertinent mechanistic questions in vivo and to assess metabolic changes in both the central nervous system and peripheral tissues in the most efficient and economical manner.

Public Health Relevance

The Physiology Core aims to promote innovative and collaborative research amongst its members by providing the basic infrastructure to assist those who wish to direct their unique expertise towards understanding the pathophysiology of diabetes and its complications using in vivo physiological approaches.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK045735-21
Application #
8446557
Study Section
Special Emphasis Panel (ZDK1-GRB-S (O2))
Project Start
Project End
Budget Start
2013-03-15
Budget End
2014-01-31
Support Year
21
Fiscal Year
2013
Total Cost
$200,606
Indirect Cost
$78,197
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Zhang, Yongdeng; Lara-Tejero, María; Bewersdorf, Jörg et al. (2017) Visualization and characterization of individual type III protein secretion machines in live bacteria. Proc Natl Acad Sci U S A 114:6098-6103
Varela, Luis; Suyama, Shigetomo; Huang, Yan et al. (2017) Endothelial HIF-1? Enables Hypothalamic Glucose Uptake to Drive POMC Neurons. Diabetes 66:1511-1520
Park, Jin-Kyu; Shao, Mingjie; Kim, Moon Young et al. (2017) An endoplasmic reticulum protein, Nogo-B, facilitates alcoholic liver disease through regulation of kupffer cell polarization. Hepatology 65:1720-1734
Thompson, Alexander D; Bewersdorf, Joerg; Toomre, Derek et al. (2017) HIDE Probes: A New Toolkit for Visualizing Organelle Dynamics, Longer and at Super-Resolution. Biochemistry 56:5194-5201
Jelenik, Tomas; Kaul, Kirti; Séquaris, Gilles et al. (2017) Mechanisms of Insulin Resistance in Primary and Secondary Nonalcoholic Fatty Liver. Diabetes 66:2241-2253
Perry, Rachel J; Peng, Liang; Abulizi, Abudukadier et al. (2017) Mechanism for leptin's acute insulin-independent effect to reverse diabetic ketoacidosis. J Clin Invest 127:657-669
Lees, Joshua A; Messa, Mirko; Sun, Elizabeth Wen et al. (2017) Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion. Science 355:
Chowdhury, Golam M I; Wang, Peili; Ciardi, Alisha et al. (2017) Impaired Glutamatergic Neurotransmission in the Ventromedial Hypothalamus May Contribute to Defective Counterregulation in Recurrently Hypoglycemic Rats. Diabetes 66:1979-1989
Sun, Emily W; de Fontgalland, Dayan; Rabbitt, Philippa et al. (2017) Mechanisms Controlling Glucose-Induced GLP-1 Secretion in Human Small Intestine. Diabetes 66:2144-2149
Lee, Hui-Young; Lee, Jae Sung; Alves, Tiago et al. (2017) Mitochondrial-Targeted Catalase Protects Against High-Fat Diet-Induced Muscle Insulin Resistance by Decreasing Intramuscular Lipid Accumulation. Diabetes 66:2072-2081

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