During the past funding period, the Cell Culture Core has continued to provide isolation of mouse and rat hepatocytes from normal and diseased livers, cell line banking, and distribution of human hepatocytes. A separately funded (through NIAAA beginning in mid-2001) Non-parenchymal Liver Cell Core provides freshly isolated and cultured normal or diseased rat Kupffer cells, hepatic stellate cells, and sinusoidal endothelial cells. Effective 9/1/04, the Cell Culture Core stopped preparing human hepatocytes but instead distributes human hepatocytes prepared by a commercial source (CellzDirect, Tucson, Az). This is because an agreement was made between the USC liver surgeons and CellzDirect, which supports the liver tissue repository at USC and provides human hepatocytes free of charge to Liver Center investigators. The Cell Culture Core has continued to provide a vital service to the Center members and has remained near steady state from the previoijs to the current grant cycle in terms of usage (number of preps, investigators and grants) and productivity (number of publications). Sixteen members and affiliated members used the Cell Culture Core from 1998 to 2003 with 47 publications. In the current cycle, 2003 to 2008, 18 full members used the Core and generated 50 publications. In particular, the Cell Culture Core has been instrumental in assisting young investigators in establishing their independent research programs (e.g. H.P. Yang, D. Han, C. Ji and Z.X. Liu).

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK048522-17
Application #
8216478
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
17
Fiscal Year
2011
Total Cost
$283,514
Indirect Cost
Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Matsushita, Noriko; Hassanein, Mohamed T; Martinez-Clemente, Marcos et al. (2017) Gender difference in NASH susceptibility: Roles of hepatocyte Ikk? and Sult1e1. PLoS One 12:e0181052
Tomita, Kyoko; Kohli, Rohit; MacLaurin, Brittany L et al. (2017) Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis. JCI Insight 2:
Elmasry, Sandra; Wadhwa, Sanya; Bang, Bo-Ram et al. (2017) Detection of Occult Hepatitis C Virus Infection in Patients Who Achieved a Sustained Virologic Response to Direct-Acting Antiviral Agents for Recurrent Infection After Liver Transplantation. Gastroenterology 152:550-553.e8
Lee, Amy S; Brandhorst, Sebastian; Rangel, Daisy F et al. (2017) Effects of Prolonged GRP78 Haploinsufficiency on Organ Homeostasis, Behavior, Cancer and Chemotoxic Resistance in Aged Mice. Sci Rep 7:40919
Sakiyama, Ryoichi; Blau, Brandon J; Miki, Toshio (2017) Clinical translation of bioartificial liver support systems with human pluripotent stem cell-derived hepatic cells. World J Gastroenterol 23:1974-1979
Alonso, Cristina; Fernández-Ramos, David; Varela-Rey, Marta et al. (2017) Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis. Gastroenterology 152:1449-1461.e7
Pyzik, Michal; Rath, Timo; Kuo, Timothy T et al. (2017) Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury. Proc Natl Acad Sci U S A 114:E2862-E2871
Moeini, Aida; Machida, Hiroko; Takiuchi, Tsuyoshi et al. (2017) Association of Nonalcoholic Fatty Liver Disease and Venous Thromboembolism in Women With Endometrial Cancer. Clin Appl Thromb Hemost 23:1018-1027
Johnson, Sandra A S; Lin, Justin J; Walkey, Christopher J et al. (2017) Elevated TATA-binding protein expression drives vascular endothelial growth factor expression in colon cancer. Oncotarget 8:48832-48845
Dhandhukia, Jugal P; Li, Zhe; Peddi, Santosh et al. (2017) Berunda Polypeptides: Multi-Headed Fusion Proteins Promote Subcutaneous Administration of Rapamycin to Breast Cancer In Vivo. Theranostics 7:3856-3872

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