and zebrafish model systems developed in the Center. These technologies, fluorescence activated ceil sorting (FACS) and epigenetic analysis on small numbers of cells, will provide an unprecedented view of hematopoiesis. The recent development of technologies such as chromatin immunoprecipitation and next generation sequencing (ChlP-seq) promises to dramatically enhance our understanding of gene expression in hematopoietic cells. CORE C will provide Center members with ready access to the latest, most advanced cell sorting capabilities, and will further develop, apply, and make widely available the most innovative approaches to epigenetic analyses on limiting numbers of primary cells. The efforts of CORE C will significantly lower the barrier to application of these technologies. Significance: A detailed understanding of blood cell development during normal hematopoiesis and various disease states has direct relevance to human disease and will aid in the development of new therapeutic approaches.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK049216-19
Application #
8565741
Study Section
Special Emphasis Panel (ZDK1-GRB-G (O3))
Project Start
1997-09-05
Project End
2015-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
19
Fiscal Year
2012
Total Cost
$313,856
Indirect Cost
$127,754
Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115
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