Cell Morphology and Pathology Core: The goals of the Cell Morphology and Pathology Core are to provide services and instruction to investigators of the Gene Therapy Center and to new investigators interested in developing gene transfer approaches to cystic fibrosis (CF) and other genetic diseases. We strive to provide expert assistance in the interpretation of disease pathology as investigators use gene transfer tools in new large and small animal models of human disease. To facilitate these goals the Core provides: 1) technical assistance for labor- intensive techniques such as the preparation of sections from tissue or cell cultures, 2) ready access to equipment, 3) economic benefits through centralization of equipment and facilities, and 4) consultation and instruction in specialized morphologic techniques and methods of image analysis. These goals are met through oversight by the Core Director, an established CF scientist with experience in a variety of microscopy techniques that are useful in gene therapy research.
The specific aims of the Cell Morphology and Pathology Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding, sectioning and routine staining for Center investigators. 2) For investigators who require detection of reporter proteins, to provide assistance with staining techniques, such as detection and cell-specific localization of (J-galactosidase activity. 3) To provide assistance with antibody labeling of proteins using histochemical and fluorescence techniques to investigators who require cell and tissue detection of specific proteins. 4) For investigators who require electron microscopy (TEM or SEM) or confocal microscopy, to provide tissue fixation, processing, and assistance in photo micrography, computerized analysis and interpretation of results. 5) For investigators who require in situ hybridization, to provide instruction and assistance in tissue preparation and detection of the probe. 6) To provide expert veterinary pathology support for the examination and interpretation of microscopic to gross specimens in animal models of disease and their response to gene-based therapies. 7) To provide assistance to investigators in the development and use of new and/or specialized morphological methods relevant to gene transfer.
|Hisert, Katherine B; Schoenfelt, Kelly Q; Cooke, Gordon et al. (2016) Ivacaftor-Induced Proteomic Changes Suggest Monocyte Defects May Contribute to the Pathogenesis of Cystic Fibrosis. Am J Respir Cell Mol Biol 54:594-7|
|Paharik, Alexandra E; Salgado-Pabon, Wilmara; Meyerholz, David K et al. (2016) The Spl Serine Proteases Modulate Staphylococcus aureus Protein Production and Virulence in a Rabbit Model of Pneumonia. mSphere 1:|
|Guevara, Claudia; Zhang, Chengxian; Gaddy, Jennifer A et al. (2016) Highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussis. Infect Dis (Lond) 48:177-88|
|Pohl, John; Morinville, Veronique; Husain, Sohail Z et al. (2016) Toxic-Metabolic Risk Factors Are Uncommon in Pediatric Chronic Pancreatitis. J Pediatr Gastroenterol Nutr 62:e66-7|
|Yang, Tian; Tal-Gan, Yftah; Paharik, Alexandra E et al. (2016) Structure-Function Analyses of a Staphylococcus epidermidis Autoinducing Peptide Reveals Motifs Critical for AgrC-type Receptor Modulation. ACS Chem Biol 11:1982-91|
|Mou, Hongmei; Vinarsky, Vladimir; Tata, Purushothama Rao et al. (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells. Cell Stem Cell 19:217-31|
|Reznikov, Leah R; Meyerholz, David K; Adam, Ryan J et al. (2016) Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice. PLoS One 11:e0166089|
|Bartlett, Jennifer A; Ramachandran, Shyam; Wohlford-Lenane, Christine L et al. (2016) Newborn Cystic Fibrosis Pigs Have a Blunted Early Response to an Inflammatory Stimulus. Am J Respir Crit Care Med 194:845-854|
|Hammond, Emily; Newell Jr, John D; Dilger, Samantha K N et al. (2016) Computed Tomography and Magnetic Resonance Imaging for Longitudinal Characterization of Lung Structure Changes in a Yucatan Miniature Pig Silicosis Model. Toxicol Pathol 44:373-81|
|Bhatt, Surya P; Bodduluri, Sandeep; Newell, John D et al. (2016) CT-derived Biomechanical Metrics Improve Agreement Between Spirometry and Emphysema. Acad Radiol 23:1255-63|
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