The Metabolism Core is a state-of-the art facility for assessing human energy expenditure, body composition,? and risk for chronic metabolic disease. It provides expertise, resources, and training in:? ? Energy expenditure (free-living energy expenditure by doubly-labeled water and isotope-ratio mass? spectrometry);? ? Body composition and fat distribution [(underwater weighing and BodPod for measurement of whole? body density; dual-energy X-ray absorptiometry (DXA) for measurement of regional and whole-body? bone, fat, and soft lean tissue; isotope dilution for assessment of total body water; multi-compartment? models of body composition; analysis of regional body composition (intra-abdominal and subcutaneous? abdominal adipose tissue from computed tomography scans)];? ? Insulin sensitivity testing (frequently-sampled, intravenous, glucose tolerance test with minimal? modeling); oral glucose tolerance test with analysis of insulin and glucose;? ? Analysis of glucose, lipids, and hormones (including obesity-related hormones, e.g., leptin, adiponectin;? diabetes-related hormones, e.g., insulin, C-peptide, glucagon, GLP-1, GIP; a reproductive-endocrine? panel, e.g., estradiol, testosterone, other steroid hormones, gonadotropins; elements of the IGF system,? e.g., IGF-1 and its binding proteins; cytokines and markers of inflammation, e.g., TNF-a and its? receptors, IL-6, CRP);? ? Analysis of isotopically-labeled glucose and amino acids by GC/MS for use in in vivo metabolic studies? (e.g., endogenous glucose production, protein turnover).? Since inception in 2000, Core A has supported 65 investigators and 102 research projects. Current core use? includes 24 investigators from 13 departments/divisions across DAB, and seven investigators from outside? of DAB. These investigators have a total of 47 funded projects that use Core services. Of these projects, 38? (81%) are federally-funded. Two CNRU P/F studies presently use the Metabolism Core.? New Methods Development includes, 1) the validation of a non-invasive test for insulin sensitivity in children? using a mixed-meal, and, 2) implementation of methodology for assessing insulin secretion and clearance? using data resulting from a mixed-meal test.
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