Core D constitutes a centralized resource for packaging retroviral expression constructs into viral particles of choice (gamma-, lenti-, and foamy viruses). Services provided by this resource include: a) scalable viral production services with viral preps ranging from 2 x 10 to 2 x 10 transducing units, b) titer assessments by either fluorescent marker tracking or by real time PCR analysis, c) consultation for investigators regarding the appropriate design of the retroviral expression construct, and d) provision of control expression constructs. Core D in collaboration with Cores C, E, and F will also investigate new strategies for marking and detecting progeny of individual stem cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK056465-14
Application #
8566005
Study Section
Special Emphasis Panel (ZDK1-GRB-G (O3))
Project Start
1999-09-15
Project End
2015-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
14
Fiscal Year
2012
Total Cost
$182,920
Indirect Cost
$76,907
Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
078200995
City
Seattle
State
WA
Country
United States
Zip Code
98109
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