The Cell Biology/Morphology Core provides individual investigators with technical support, equipment, and expertise for cell biological, morphological and immunocytochemical studies related to the mission of the BADERC grant. The expertise of the PI and his colleagues spans a wide range of imaging procedures, from basic and confocal light/fluorescence microscopic analysis of antigen localization, to high resolution electron microscopy and immunogold labeling on ultrathin frozen sections of cells and tissues, and quantitative live cell imaging and ratiometric imaging. The Core will, as a major objective, provide an environment appropriate for the training of key personnel from participating laboratories. The rationale for the Core lies in the necessity to provide an integrated approach to problems in Diabetes and other designated research areas, in an era when no single laboratory can develop the highest level of technical competence in several important and specialized areas. In addition, the Cell Biology Core is designed to maximize the use and availability of several specialized items of equipment, that are not only prohibitively expensive for many laboratories, but that also require expensive and regular maintenance. The techniques offered by the Core require a high level of sophisticated technology that is constantly being updated to maintain state-of-the-art performance. Thus, investigators are able to explore the use of a varied and constantly expanding arsenal of methods in order to achieve success in their studies. These procedures are an essential part of the modern multidisciplinary approach to cell biological questions as they relate to Diabetes and Endocrinology.

Public Health Relevance

This Core facility provides access to spohisticated equipment that is necessary to help individual labs and investigators pursue their work on studies related to diabetes and endocrinology. The large and expensive microscopy equipment is largely beyond the budget and expertize of individual investigstors to purchase and maintain and this Core, therefore, provides a valuable and unique resource to the BADERC community.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-2)
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Massachusetts General Hospital
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Krashes, Michael J; Shah, Bhavik P; Madara, Joseph C et al. (2014) An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature 507:238-42
Nomura, Naohiro; Nunes, Paula; Bouley, Richard et al. (2014) High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration. Am J Physiol Cell Physiol 307:C597-605
Lee, Seung-Hwan; Huang, Hu; Choi, Kangduk et al. (2014) ROCK1 isoform-specific deletion reveals a role for diet-induced insulin resistance. Am J Physiol Endocrinol Metab 306:E332-43
Yuen, Grace J; Ausubel, Frederick M (2014) Enterococcus infection biology: lessons from invertebrate host models. J Microbiol 52:200-10
Chiappini, Franck; Catalano, Karyn J; Lee, Jennifer et al. (2014) Ventromedial hypothalamus-specific Ptpn1 deletion exacerbates diet-induced obesity in female mice. J Clin Invest 124:3781-92
Cohen, Paul; Levy, Julia D; Zhang, Yingying et al. (2014) Ablation of PRDM16 and beige adipose causes metabolic dysfunction and a subcutaneous to visceral fat switch. Cell 156:304-16
Wei, Nancy; Pan, Jessica; Pop-Busui, Rodica et al. (2014) Altered sphingoid base profiles in type 1 compared to type 2 diabetes. Lipids Health Dis 13:161
Kraus, Daniel; Yang, Qin; Kong, Dong et al. (2014) Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature 508:258-62
Oshiro, Noriko; Rapley, Joseph; Avruch, Joseph (2014) Amino acids activate mammalian target of rapamycin (mTOR) complex 1 without changing Rag GTPase guanyl nucleotide charging. J Biol Chem 289:2658-74
Ruan, Ye Chun; Wang, Yan; Da Silva, Nicolas et al. (2014) CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation through the ZONAB pathway. J Cell Sci 127:4396-408

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