The cloning and engineering of genetically encoded fluorescent proteins has allowed the development of many unique tools for studies in cell biology, medicine, and physiology. When combined with intravital microscopy, these probes have the potential to report events from inside living cells in the intact organism. However, the utility of the fluorescent protein biosensors for intravital microscopy has been limited by the poorly characterized behaviors of fluorescent proteins under two-photon excitation. The goal of the Multiphoton Biosensor Development core is to develop and rigorously validate a set of biosensor probes optimized for intravital multiphoton microscopy studies of renal function. Modular probes developed by the core will be incorporated into a toolkit of probes and protocols that will be provided to renal investigators. These probes will provide renal researchers with powerful new tools that will enable them to conduct novel intravital microscopy studies addressing fundamental issues of renal physiology, cell biology and pathophysiology.

Public Health Relevance

The Indiana O'Brien Center is founded upon the mission of developing and implementing methods of microscopy that provide unique and powerful insights into renal function and dysfunction. The Multi-photon Biosensor Development Core will play a critical role in this mission, developing novel biosensors that will support unique microscopy studies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK079312-08
Application #
8726960
Study Section
Special Emphasis Panel (ZDK1-GRB-6)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
8
Fiscal Year
2014
Total Cost
$157,806
Indirect Cost
$56,648
Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
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