The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. Core C has been successful in supporting the kidney research community. Since the inception of Core C, more than 8,500 assays have been performed for 48 principal investigators involving 56 projects. Of the 48 investigators, 44 were non-core personnel. The number of investigators using Core C each year is increasing, as is the annual publication rate. Core C has also supported the research efforts of 7 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 23 peer-reviewed publications. The Core will participate in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature from 2013-2017 will be an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics). Finally, the Bioanalytical Core, in concert with the O'Brien Center leadership, will identify new bioanalytical needs of investigators and implement them for the Center.

Public Health Relevance

Acute kidney injury is associated with severe consequences including death, prolonged hospitalization and can lead to chronic kidney disease. Core C of this Center provides investigators key resources required to enable a better understanding of the underlying mechanisms and determine best approaches to diagnose and manage this disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK079337-07
Application #
8733667
Study Section
Special Emphasis Panel (ZDK1-GRB-6)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
7
Fiscal Year
2014
Total Cost
$231,244
Indirect Cost
$51,626
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Boddu, Ravindra; Fan, Chunlan; Rangarajan, Sunil et al. (2017) Unique sex- and age-dependent effects in protective pathways in acute kidney injury. Am J Physiol Renal Physiol 313:F740-F755
Bolisetty, Subhashini; Zarjou, Abolfazl; Agarwal, Anupam (2017) Heme Oxygenase 1 as a Therapeutic Target in Acute Kidney Injury. Am J Kidney Dis 69:531-545
Balena-Borneman, Jessica; Ambalavanan, Namasivayam; Tiwari, Hemant K et al. (2017) Biomarkers associated with bronchopulmonary dysplasia/mortality in premature infants. Pediatr Res 81:519-525
Sun, Yong; Byon, Chang Hyun; Yang, Youfeng et al. (2017) Dietary potassium regulates vascular calcification and arterial stiffness. JCI Insight 2:
Soni, Hitesh; Adebiyi, Adebowale (2017) Early septic insult in neonatal pigs increases serum and urinary soluble Fas ligand and decreases kidney function without inducing significant renal apoptosis. Ren Fail 39:83-91
Leaf, David E; Jacob, Kirolos A; Srivastava, Anand et al. (2017) Fibroblast Growth Factor 23 Levels Associate with AKI and Death in Critical Illness. J Am Soc Nephrol 28:1877-1885
Pike, Daniel; Shiu, Yan-Ting; Somarathna, Maheshika et al. (2017) High resolution hemodynamic profiling of murine arteriovenous fistula using magnetic resonance imaging and computational fluid dynamics. Theor Biol Med Model 14:5
Cerdá, Jorge; Mohan, Sumit; Garcia-Garcia, Guillermo et al. (2017) Acute Kidney Injury Recognition in Low- and Middle-Income Countries. Kidney Int Rep 2:530-543
Alasmari, Fawaz; Crotty Alexander, Laura E; Nelson, Jessica A et al. (2017) Effects of chronic inhalation of electronic cigarettes containing nicotine on glial glutamate transporters and ?-7 nicotinic acetylcholine receptor in female CD-1 mice. Prog Neuropsychopharmacol Biol Psychiatry 77:1-8
Prasain, J K; Wilson, L S; Arabshahi, A et al. (2017) Mass spectrometric evidence for the modification of small molecules in a cobalt-60-irradiated rodent diet. J Mass Spectrom 52:507-516

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