In vivo methods in both humas and animals have proven valuable in elucidating the pathophysiology of diabetes and cardiometabolic disease, despite some technological limitations. The Animal Physiology Core (APC) has incorporated recent progress in the miniaturization of instrumentationand and increased sensitivity of monitoring devices to provide tools for measurements in intact undisturbed rodents. The APC brings together four experts in the areas of integrative physiology, cardiovascular physiology, imaging, and animal models who serve as a core resource for the study of diabetes using rodent models. The goal of the APC is to provide easy access to highly-specialized equipment and expertise in the area of body composition, energetics, glucose homeostasis, cardiovascular assessment, imaging, and transgenic animal models and technology, to augment diabetes and cardiometabolic research quality and cost effectiveness.
The Specific Aims of the Core are: 1. To provide expertise in the use of animal models for diabetes and cardiovascular research; 2. To provide state-of-the-art instrumentation and methodology for the determination of: a. Body composition: Whole-body composition analysis by chemical carcass analysis, dual-energy X-ray absorptiometry (DXA), quantitative magnetic resonance (QMR), Faxitron X-ray, and micro-computed tomography (pCT). b. Energy balance: Comprehensive assessments of metabolic rate (indirect calorimetry), food intake, fecal output, activity, and body temperature. c. Glucose homeostasis: Glucose and insulin tolerance testing and hyperinsulinemic-euglycemic clamps. d. Cardiovascular assessment: Echocardiography and blood pressure. e. Imaging: Bioluminescence, fluorescence, and gamma-ray imaging including SPECT, PET, and MRI. f. Transgenic animal models: Assistance with construct preparation, generation of transgenic/knock-out mice, husbandry and colony management, and genotyping.
The high quality, breadth, and cutting-edge nature of the Core's technologies, and responsiveness to the evolving needs of our investigators, have resulted in high rates of utilization by the DRC research base. By promulgating high quality services in small animal phenotyping, the Core is an important strength for assuring that research in the DRC is promoted across the full spectrum of translational research.
|Misra, Devyani; Felson, David T; Silliman, Rebecca A et al. (2015) Knee osteoarthritis and frailty: findings from the Multicenter Osteoarthritis Study and Osteoarthritis Initiative. J Gerontol A Biol Sci Med Sci 70:339-44|
|Panwar, Bhupesh; Hanks, Lynae J; Tanner, Rikki M et al. (2015) Obesity, metabolic health, and the risk of end-stage renal disease. Kidney Int 87:1216-22|
|Casazza, Krista; Brown, Andrew; Astrup, Arne et al. (2015) Weighing the Evidence of Common Beliefs in Obesity Research. Crit Rev Food Sci Nutr 55:2014-53|
|Harper, Lorie M; Tita, Alan; Biggio, Joseph R (2015) The institute of medicine guidelines for gestational weight gain after a diagnosis of gestational diabetes and pregnancy outcomes. Am J Perinatol 32:239-46|
|Cree-Green, Melanie; Newcomer, Bradley R; Brown, Mark S et al. (2015) Delayed skeletal muscle mitochondrial ADP recovery in youth with type 1 diabetes relates to muscle insulin resistance. Diabetes 64:383-92|
|Bone, Robert N; Gai, Ying; Magrioti, Victoria et al. (2015) Inhibition of Ca2+-independent phospholipase A2? (iPLA2?) ameliorates islet infiltration and incidence of diabetes in NOD mice. Diabetes 64:541-54|
|Carson, April P; Steffes, Michael W; Carr, J Jeffrey et al. (2015) Hemoglobin a1c and the progression of coronary artery calcification among adults without diabetes. Diabetes Care 38:66-71|
|Clement, Lionel C; Macé, Camille; Del Nogal Avila, Maria et al. (2015) The proteinuria-hypertriglyceridemia connection as a basis for novel therapeutics for nephrotic syndrome. Transl Res 165:499-504|
|Hanks, Lynae J; Casazza, Krista; Ashraf, Ambika P et al. (2015) Fibroblast growth factor-21, body composition, and insulin resistance in pre-pubertal and early pubertal males and females. Clin Endocrinol (Oxf) 82:550-6|
|Armstrong, Anderson C; Liu, Kiang; Lewis, Cora E et al. (2014) Left atrial dimension and traditional cardiovascular risk factors predict 20-year clinical cardiovascular events in young healthy adults: the CARDIA study. Eur Heart J Cardiovasc Imaging 15:893-9|
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