In vivo methods in both humas and animals have proven valuable in elucidating the pathophysiology of diabetes and cardiometabolic disease, despite some technological limitations. The Animal Physiology Core (APC) has incorporated recent progress in the miniaturization of instrumentationand and increased sensitivity of monitoring devices to provide tools for measurements in intact undisturbed rodents. The APC brings together four experts in the areas of integrative physiology, cardiovascular physiology, imaging, and animal models who serve as a core resource for the study of diabetes using rodent models. The goal of the APC is to provide easy access to highly-specialized equipment and expertise in the area of body composition, energetics, glucose homeostasis, cardiovascular assessment, imaging, and transgenic animal models and technology, to augment diabetes and cardiometabolic research quality and cost effectiveness.
The Specific Aims of the Core are: 1. To provide expertise in the use of animal models for diabetes and cardiovascular research; 2. To provide state-of-the-art instrumentation and methodology for the determination of: a. Body composition: Whole-body composition analysis by chemical carcass analysis, dual-energy X-ray absorptiometry (DXA), quantitative magnetic resonance (QMR), Faxitron X-ray, and micro-computed tomography (pCT). b. Energy balance: Comprehensive assessments of metabolic rate (indirect calorimetry), food intake, fecal output, activity, and body temperature. c. Glucose homeostasis: Glucose and insulin tolerance testing and hyperinsulinemic-euglycemic clamps. d. Cardiovascular assessment: Echocardiography and blood pressure. e. Imaging: Bioluminescence, fluorescence, and gamma-ray imaging including SPECT, PET, and MRI. f. Transgenic animal models: Assistance with construct preparation, generation of transgenic/knock-out mice, husbandry and colony management, and genotyping.

Public Health Relevance

The high quality, breadth, and cutting-edge nature of the Core's technologies, and responsiveness to the evolving needs of our investigators, have resulted in high rates of utilization by the DRC research base. By promulgating high quality services in small animal phenotyping, the Core is an important strength for assuring that research in the DRC is promoted across the full spectrum of translational research.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30DK079626-07
Application #
8640160
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
DUNS #
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Jones, Kristian T; Shelton, Richard C; Wan, Jun et al. (2016) Impact of acute psychological stress on cardiovascular risk factors in face of insulin resistance. Stress 19:585-592
Wende, Adam R; Young, Martin E; Chatham, John et al. (2016) Redox biology and the interface between bioenergetics, autophagy and circadian control of metabolism. Free Radic Biol Med 100:94-107
Goldsby, TaShauna U; George, Brandon J; Yeager, Valerie A et al. (2016) Urban Park Development and Pediatric Obesity Rates: A Quasi-Experiment Using Electronic Health Record Data. Int J Environ Res Public Health 13:411
Hong, Kyunghee; Xu, Guanlan; Grayson, Truman B et al. (2016) Cytokines Regulate β-Cell Thioredoxin-interacting Protein (TXNIP) via Distinct Mechanisms and Pathways. J Biol Chem 291:8428-39
Howard, George; Moy, Claudia S; Howard, Virginia J et al. (2016) Where to Focus Efforts to Reduce the Black-White Disparity in Stroke Mortality: Incidence Versus Case Fatality? Stroke 47:1893-8
Sweatt, S Katherine; Roy, Jane; Chandler-Laney, Paula et al. (2016) Ethnic differences in the consistency and accuracy of perceived exertion. Am J Hum Biol 28:398-404
Cunningham, Glenn R; Stephens-Shields, Alisa J; Rosen, Raymond C et al. (2016) Testosterone Treatment and Sexual Function in Older Men With Low Testosterone Levels. J Clin Endocrinol Metab 101:3096-104
Cardel, M I; Johnson, S L; Beck, J et al. (2016) The effects of experimentally manipulated social status on acute eating behavior: A randomized, crossover pilot study. Physiol Behav 162:93-101
Krzywanski, David M; Moellering, Douglas R; Westbrook, David G et al. (2016) Endothelial Cell Bioenergetics and Mitochondrial DNA Damage Differ in Humans Having African or West Eurasian Maternal Ancestry. Circ Cardiovasc Genet 9:26-36
Quon, Bradley S; Rowe, Steven M (2016) New and emerging targeted therapies for cystic fibrosis. BMJ 352:i859

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