The Transgenic and ES Cell Core of the JHU-UMD DRC will enable Members at Johns Hopkins University and the University of Maryland to efficiently generate transgenic and knock-out/in mice. Fredric E. Wondisford, M.D. will serve as Director, and Sally Radovick, M.D. will serve as Co-Director. Both are highly qualified to direct this core and their direct involvement in diabetes and diabetes-related research should further enhance transgenic/knock-out services to JHU-UMD investigators. The Center will utilize the existing Transgenic Core at Johns Hopkins University School of Medicine with the addition of dedicated technical support to expedite generation of mouse models for DRC Members and at a lesser cost. The DRC will continue to generate targeted ES cells using highly reliable and efficient protocols. The intellectual aspects associated with the generation of transgenic and knock-out/knock-in constructs and the reagents necessary for ES cells generation have proved to important for many investigators. By concentrating experience and expertise in this Core facility, the Core will markedly accelerate the progress of discovery for Center investigators. Transgenic services will be located in JHU space that is already allocated to the existing facility, and this facility has adequate equipment. ES cell services will be located in the connected CMSC building, adjacent to Dr. Wondisford's laboratories, in 500 sq. ft. of space. Drs. Wondisford and Radovick will assist in the design of targeting constructs. The Core will be managed by Dr. Sidhaye, who will oversee the introduction of constructs into ES cells, identifying ES clones that have undergone homologous recombination along with the respective investigator's laboratory and preparation of cells for injection into blastocysts. Facilitating transport of genetically altered mice from JHU to UMD has been part of the Core's function. Alternatively, budgeted into the Core'are short-term housing costs at JHU for mice made for UMD Members, if their study protocol requires imaging or metabolic studies.
The Transgenic and ES Cell Core of the JHU-UMD DRC enables Members at Johns Hopkins University and the University of Maryland to efficiently generate transgenic and knock-out/in mice for use in the study of diabetes mechanisms. This proposal seeks funding to continue this core acitivty.
|Shin, Mi-Kyung; Han, Woobum; Bevans-Fonti, Shannon et al. (2014) The effect of adrenal medullectomy on metabolic responses to chronic intermittent hypoxia. Respir Physiol Neurobiol 203:60-7|
|Min, Jungwon; Li, Zhu; Liu, Xia et al. (2014) The association between early menarche and offspring's obesity risk in early childhood was modified by gestational weight gain. Obesity (Silver Spring) 22:19-23|
|Kuang, Zheng; Cai, Ling; Zhang, Xuekui et al. (2014) High-temporal-resolution view of transcription and chromatin states across distinct metabolic states in budding yeast. Nat Struct Mol Biol 21:854-63|
|Ivey, Frederick M; Ryan, Alice S (2014) Resistive training improves insulin sensitivity after stroke. J Stroke Cerebrovasc Dis 23:225-9|
|Gabelli, Sandra B; Echeverria, Ignacia; Alexander, Megan et al. (2014) Activation of PI3K? by physiological effectors and by oncogenic mutations: structural and dynamic effects. Biophys Rev 6:89-95|
|Chatterjee, Ranee; Brancati, Frederick L; Shafi, Tariq et al. (2014) Non-traditional risk factors are important contributors to the racial disparity in diabetes risk: the atherosclerosis risk in communities study. J Gen Intern Med 29:290-7|
|Albert, Jessica S; Yerges-Armstrong, Laura M; Horenstein, Richard B et al. (2014) Null mutation in hormone-sensitive lipase gene and risk of type 2 diabetes. N Engl J Med 370:2307-15|
|Zhang, Tao; Baldie, Gerard; Periz, Goran et al. (2014) RNA-processing protein TDP-43 regulates FOXO-dependent protein quality control in stress response. PLoS Genet 10:e1004693|
|Cortassa, Sonia; O'Rourke, Brian; Aon, Miguel A (2014) Redox-optimized ROS balance and the relationship between mitochondrial respiration and ROS. Biochim Biophys Acta 1837:287-95|
|Xie, Hui; Cui, Zhuang; Wang, Long et al. (2014) PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis. Nat Med 20:1270-8|
Showing the most recent 10 out of 125 publications