The Optical Morphology Core will play an essential role in supporting the overall goals of the Center.
The Specific Aims of this core are three-fold. First, to provide reliable, accessible, and state-of-the-art microscopic technology to all Center members that will facilitate their study of Gl cellular signaling cascades. Second, to educate and train Center members in the use of both basic and sophisticated cellular imaging methods. Emphasis will be placed on providing technical instruction as well as educating faculty about how such approaches can expand the scope and breadth of their scientific programs. Third, development and application of state-of-the-art optical imaging technologies to Gl tissues/cells including;high resolution, real-time computer/video imaging of live cells;confocal microscopy coupled with computer-based 3-D image reconstruction;Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein-protein interactions;Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover;expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids;and the development and application of specific photoactivatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK084567-05
Application #
8517109
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
5
Fiscal Year
2013
Total Cost
$210,316
Indirect Cost
$71,126
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
006471700
City
Rochester
State
MN
Country
United States
Zip Code
55905
Yang, Liu; Kwon, Junghee; Popov, Yury et al. (2014) Vascular endothelial growth factor promotes fibrosis resolution and repair in mice. Gastroenterology 146:1339-50.e1
Guenzel, Adam J; Hillestad, Matthew L; Matern, Dietrich et al. (2014) Effects of adeno-associated virus serotype and tissue-specific expression on circulating biomarkers of propionic acidemia. Hum Gene Ther 25:837-43
Bi, Yan; Mukhopadhyay, Dhriti; Drinane, Mary et al. (2014) Endocytosis of collagen by hepatic stellate cells regulates extracellular matrix dynamics. Am J Physiol Cell Physiol 307:C622-33
White, Thomas A; LeBrasseur, Nathan K (2014) Myostatin and sarcopenia: opportunities and challenges - a mini-review. Gerontology 60:289-93
Peng, Ying; Clark, Karl J; Campbell, Jarryd M et al. (2014) Making designer mutants in model organisms. Development 141:4042-54
Yaqoob, Usman; Jagavelu, Kumaravelu; Shergill, Uday et al. (2014) FGF21 promotes endothelial cell angiogenesis through a dynamin-2 and Rab5 dependent pathway. PLoS One 9:e98130
Razumilava, Nataliya; Gradilone, Sergio A; Smoot, Rory L et al. (2014) Non-canonical Hedgehog signaling contributes to chemotaxis in cholangiocarcinoma. J Hepatol 60:599-605
Koh, Kwi Hye; Pan, Xian; Zhang, Wei et al. (2014) Kr├╝ppel-like factor 9 promotes hepatic cytochrome P450 2D6 expression during pregnancy in CYP2D6-humanized mice. Mol Pharmacol 86:727-35
Tabibian, James H; O'Hara, Steven P; Splinter, Patrick L et al. (2014) Cholangiocyte senescence by way of N-ras activation is a characteristic of primary sclerosing cholangitis. Hepatology 59:2263-75
Bhat, Mamatha; Chaiteerakij, Roongruedee; Harmsen, William S et al. (2014) Metformin does not improve survival in patients with hepatocellular carcinoma. World J Gastroenterol 20:15750-5

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