The DDBTRCC Imaging Core B is designed to provide multiple tj^jes of cutting edge fluorescence microscopy and electron microscopy (EM) techniques to study the physiology ofepithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles to our Research Base investigators. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca measurements at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. Each microscope is equipped with temperature controlled perfusion chambers either for tissue or for cell culture studies. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorometer) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation.
The DDBTRCC Imaging Core provides advanced Imaging (confocal and two-photon microscopy with FRAP, FRET, TIRF) and routine imaging (fluorescence of polarized epithelial and symmetric cells studied at the single cell or whole organ level by fluorometer or microscopy based systems in which access to apical and basolateral surface can be accomplished) plus EM to our Research Base to allow characterization of the structural aspects of their projects.
|Bettridge, John; Na, Chan Hyun; Pandey, Akhilesh et al. (2017) H3K4me3 induces allosteric conformational changes in the DNA-binding and catalytic regions of the V(D)J recombinase. Proc Natl Acad Sci U S A 114:1904-1909|
|Blutt, Sarah E; Broughman, James R; Zou, Winnie et al. (2017) Gastrointestinal microphysiological systems. Exp Biol Med (Maywood) 242:1633-1642|
|Sunuwar, Laxmi; Asraf, Hila; Donowitz, Mark et al. (2017) The Zn2+-sensing receptor, ZnR/GPR39, upregulates colonocytic Cl- absorption, via basolateral KCC1, and reduces fluid loss. Biochim Biophys Acta 1863:947-960|
|Jin, Peng; Wang, De-Zhi; Lyu, Chen-Xi et al. (2017) Mismatch Repair Protein hMLH1, but not hMSH2, Enhances Estrogen-Induced Apoptosis of Colon Cancer Cells. J Cancer 8:3232-3241|
|Anderson, Karen L; Page, Christopher; Swift, Mark F et al. (2017) Nano-scale actin-network characterization of fibroblast cells lacking functional Arp2/3 complex. J Struct Biol 197:312-321|
|Li, Ling; Piontek, Klaus; Ishida, Masaharu et al. (2017) Extracellular vesicles carry microRNA-195 to intrahepatic cholangiocarcinoma and improve survival in a rat model. Hepatology 65:501-514|
|Hosoda, Waki; Chianchiano, Peter; Griffin, James F et al. (2017) Genetic analyses of isolated high-grade pancreatic intraepithelial neoplasia (HG-PanIN) reveal paucity of alterations in TP53 and SMAD4. J Pathol 242:16-23|
|Cha, Boyoung; Yang, Jianbo; Singh, Varsha et al. (2017) PDZ domain-dependent regulation of NHE3 protein by both internal Class II and C-terminal Class I PDZ-binding motifs. J Biol Chem 292:8279-8290|
|Cil, Onur; Phuan, Puay-Wah; Son, Jung-Ho et al. (2017) Phenylquinoxalinone CFTR activator as potential prosecretory therapy for constipation. Transl Res 182:14-26.e4|
|Becker, Laren; Nguyen, Linh; Gill, Jaspreet et al. (2017) Age-dependent shift in macrophage polarisation causes inflammation-mediated degeneration of enteric nervous system. Gut :|
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