The Proteomics Facility Core provides analytical proteomics services to investigators in the P30 EHS Core Center Analytical proteomics integrates tools for protein and peptide separations, mass spectrometry (MS) analysis, and bioinformatics to characterize proteomes and their component proteins {1-3). Proteomics is the study of proteins and proteomes, the functional complements to genomes which comprise diverse structural and functional multiprotein machines (3). Proteomics approaches offer new tools for the molecular analysis of biological systems, the identification of therapeutic targets and mechanisms, and the identification of markers for disease and therapeutic response. In the context of this P30 EHS Core Center, the Proteomics Facility Core provides essential support for the characterization of chemical modification of proteins, including protein adducts derived from xenobiotics and reactive intermediates generated in situ, as well as regulatory post-translational modifications (4). The Proteomics Facility Core also provides essential support for the identification of interacting partners of proteins in multiprotein complexes, as well as analyses of complex proteomes and subproteomes. Research Interactions The Proteomics Core also provides a research technology platform for more elaborate, challenging, and advanced studies that require customized experimental designs, analytical approaches, and expertise. These "research functions" of the core are defined as those analytical tasks that fall outside the usual scope of the fee-for-service functions and require the sustained interaction of a Center Investigator's laboratory with a Proteomics Associate Director and skilled staff supported by the Proteomics Facility Core component of the Center grant. Because the following research services may involve an open-ended commitment of time by Core faculty, professional effort will be recorded and traced to individual projects so that no single investigator places an undue burden on the professional staff in excess of the support provided by the P30 EHS Core Center For examples of past research interactions of Center Investigators in the Proteomics Facility Core, see the Progress Report section, pp. 304-311.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES000267-47
Application #
8651460
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
47
Fiscal Year
2014
Total Cost
$140,492
Indirect Cost
$50,433
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Aboud, Asad A; Tidball, Andrew M; Kumar, Kevin K et al. (2015) PARK2 patient neuroprogenitors show increased mitochondrial sensitivity to copper. Neurobiol Dis 73:204-12
Mullins, Elwood A; Rubinson, Emily H; Eichman, Brandt F (2014) The substrate binding interface of alkylpurine DNA glycosylase AlkD. DNA Repair (Amst) 13:50-4
Davies, Sean S; Guo, Lilu (2014) Lipid peroxidation generates biologically active phospholipids including oxidatively N-modified phospholipids. Chem Phys Lipids 181:1-33
Stavros, Kallie M; Hawkins, Edward K; Rizzo, Carmelo J et al. (2014) Base-displaced intercalation of the 2-amino-3-methylimidazo[4,5-f]quinolone N2-dG adduct in the NarI DNA recognition sequence. Nucleic Acids Res 42:3450-63
Ehrmann, David C; Rose, Kristie; Calcutt, M Wade et al. (2014) Glutathionylated ?G and ?A subunits of hemoglobin F: a novel post-translational modification found in extremely premature infants by LC-MS and nanoLC-MS/MS. J Mass Spectrom 49:178-83
Liu, Wei; Xu, Libin; Lamberson, Connor et al. (2014) A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome. J Lipid Res 55:329-37
Bowman, Aaron B; Aschner, Michael (2014) Considerations on manganese (Mn) treatments for in vitro studies. Neurotoxicology 41:141-2
Brinkhaus, Sigrid Grosse; Bornhorst, Julia; Chakraborty, Sudipta et al. (2014) Elemental bioimaging of manganese uptake in C. elegans. Metallomics 6:617-21
Lamberson, Connor R; Xu, Libin; Muchalski, Hubert et al. (2014) Unusual kinetic isotope effects of deuterium reinforced polyunsaturated fatty acids in tocopherol-mediated free radical chain oxidations. J Am Chem Soc 136:838-41
Petrova, Katya V; Millsap, Amy D; Stec, Donald F et al. (2014) Characterization of the deoxyguanosine-lysine cross-link of methylglyoxal. Chem Res Toxicol 27:1019-29

Showing the most recent 10 out of 555 publications