7C. GENOMICS AND IMAGING FACILITIES CORE INTRODUCTION AND SPECIFIC AIMS The CEHS Genomics and Bioinformatics Facilities Core was created in September 2001 to provide CEHS members with integrated facilities for high-throughput, data-intensive genomics as well as bioinformatics analysis, large-scale database storage and management, data mining and data modeling required to fully implement systems approaches to studying the biological impact of environmental factors. In response to the evolution of Center member research needs, a new CEHS Imaging Facility was integrated with the CEHS Genomics and Bioinformatics Facilities Core last year, and this CEHS facilities core has been renamed the "Genomics and Imaging Facilities Core." The Core now provides CEHS members with the tools and expertise for high-resolution and high-throughput imaging integrated with data-intensive genomics and bioinformatics. This powerful combination is coupled with flexible and accessible data sharing and a strong commitment to new technology development and deployment to keep the CEHS at the cutting edge of high-throughput imaging and analytical methods. The Genomics and Imaging Facilities Core operates as both a training resource and as a service and developmental laboratory, with the following Specific Aims: (1) To provide integrated support for genomics technologies including massively parallel sequencing and oligonucleotide arrays. (2) To provide customized imaging options and the resources for high-resolution images and high-throughput screening using imaging capabilities. (3) To provide integrative support for high-throughput screening of factors with environmental impact on cellular function, including support for flexible automated fluidics, highly parallel real-time PCR, fluorescence, luminescence, absorbance, and single-cell imaging and quantification. (4) To support the bioinformatic frameworks necessary to interpret and manage these large data sets. (5) To provide and maintain network servers and storage devices to facilitate the transfer and analysis of information-rich data sets. (6) To provide training with new genomic and imaging technologies as well as commercial and open-source bioinformatics software. (7) To work with Center members to develop and acquire new technologies and to apply their results to develop data models and quality control procedures and error modeling.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES002109-34
Application #
8650837
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
34
Fiscal Year
2014
Total Cost
$238,941
Indirect Cost
$84,012
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Mannion, Anthony; Shen, Zeli; Feng, Yan et al. (2016) Draft Genome Sequences of Five Novel Polyketide Synthetase-Containing Mouse Escherichia coli Strains. Genome Announc 4:
Shabab, Mohammed; Arnold, Markus F F; Penterman, Jon et al. (2016) Disulfide cross-linking influences symbiotic activities of nodule peptide NCR247. Proc Natl Acad Sci U S A 113:10157-62
Milani, Pamela; Escalante-Chong, Renan; Shelley, Brandon C et al. (2016) Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells. Sci Rep 6:25474
Din, M Omar; Danino, Tal; Prindle, Arthur et al. (2016) Synchronized cycles of bacterial lysis for in vivo delivery. Nature 536:81-5
Yu, Amy Marie; Calvo, Jennifer A; Muthupalani, Suresh et al. (2016) The Mbd4 DNA glycosylase protects mice from inflammation-driven colon cancer and tissue injury. Oncotarget 7:28624-36
Manley, Leigh J; Ma, Duanduan; Levine, Stuart S (2016) Monitoring Error Rates In Illumina Sequencing. J Biomol Tech 27:125-128
Woods, Stephanie E; Ek, Courtney; Shen, Zeli et al. (2016) Male Syrian Hamsters Experimentally Infected with Helicobacter spp. of the H. bilis Cluster Develop MALT-Associated Gastrointestinal Lymphomas. Helicobacter 21:201-17
Spencer, Sarah J; Tamminen, Manu V; Preheim, Sarah P et al. (2016) Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers. ISME J 10:427-36
Mackos, A R; Galley, J D; Eubank, T D et al. (2016) Social stress-enhanced severity of Citrobacter rodentium-induced colitis is CCL2-dependent and attenuated by probiotic Lactobacillus reuteri. Mucosal Immunol 9:515-26
Zimanyi, Christina M; Chen, Percival Yang-Ting; Kang, Gyunghoon et al. (2016) Molecular basis for allosteric specificity regulation in class Ia ribonucleotide reductase from Escherichia coli. Elife 5:e07141

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