The functions of this Module have evolved over the years in response to changing technology and changing needs. At the beginning of the current cycle the 'molecular biology'component of the Module was very active, notably the Module's sequencing service. However, since sequencing is now largely outsourced and the research of our group increasingly emphasizes protein biochemistry, the functions of our Module now emphasize this activity, while maintaining some molecular biology support services. The major functions of the Module are twofold: (1) to provide access to and training in the use of shared-use instruments for biochemical and molecular analyses that are well maintained, and (2) to provide skilled technical support to facilitate access to the school's core research resources. The benefit to users of the first function include not only the consistent readiness of equipment, but also the savings in space, in training time, and in expense by avoiding unnecessary equipment duplication. Regarding the second Module function, facilitating access to institutional resources by our Core Grant was a strategic decision of the Core Advisory Committee during the current cycle (as indicated in the Overview section). This was seen as a way to increase innovation in our labs by making available state-of-the-art research instruments. It also effectively leverages our Core resources and reduces expenditures by Core investigators who want to use fee-for-service institutional cores. Additionally, it has the intangible but important benefit of integrating eye/vision scientists with the broader scientific community on campus. The Biochemistry-Molecular Biology Module specifically increases access to three MCW core resources: the Innovation Center (Mass Spectrometry Facility), the Biochemistry facility for Surface Plasmon Resonance (BIAcore 3000) analysis, and the Free Radical Research Center.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY001931-37
Application #
8502502
Study Section
Special Emphasis Panel (ZEY1-VSN)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
37
Fiscal Year
2013
Total Cost
$126,944
Indirect Cost
$43,974
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Kalitzeos, Angelos; Samra, Ranjit; Kasilian, Melissa et al. (2017) CELLULAR IMAGING OF THE TAPETAL-LIKE REFLEX IN CARRIERS OF RPGR-ASSOCIATED RETINOPATHY. Retina :
Baghaie, Ahmadreza; Yu, Zeyun; D'Souza, Roshan M (2017) Involuntary eye motion correction in retinal optical coherence tomography: Hardware or software solution? Med Image Anal 37:129-145
Lewis, Tylor R; Kundinger, Sean R; Pavlovich, Amira L et al. (2017) Cos2/Kif7 and Osm-3/Kif17 regulate onset of outer segment development in zebrafish photoreceptors through distinct mechanisms. Dev Biol 425:176-190
Vogel, Ryan N; Langlo, Christopher S; Scoles, Drew et al. (2017) High-Resolution Imaging of Intraretinal Structures in Active and Resolved Central Serous Chorioretinopathy. Invest Ophthalmol Vis Sci 58:42-49
Weh, Eric; Takeuchi, Hideyuki; Muheisen, Sanaa et al. (2017) Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome. PLoS One 12:e0184903
Cunefare, David; Fang, Leyuan; Cooper, Robert F et al. (2017) Open source software for automatic detection of cone photoreceptors in adaptive optics ophthalmoscopy using convolutional neural networks. Sci Rep 7:6620
Mainali, Laxman; Raguz, Marija; O'Brien, William J et al. (2017) Changes in the Properties and Organization of Human Lens Lipid Membranes Occurring with Age. Curr Eye Res 42:721-731
Protas, Meredith E; Weh, Eric; Footz, Tim et al. (2017) Mutations of conserved non-coding elements of PITX2 in patients with ocular dysgenesis and developmental glaucoma. Hum Mol Genet 26:3630-3638
Tanna, Preena; Kasilian, Melissa; Strauss, Rupert et al. (2017) Reliability and Repeatability of Cone Density Measurements in Patients With Stargardt Disease and RPGR-Associated Retinopathy. Invest Ophthalmol Vis Sci 58:3608-3615
Salmon, Alexander E; Cooper, Robert F; Langlo, Christopher S et al. (2017) An Automated Reference Frame Selection (ARFS) Algorithm for Cone Imaging with Adaptive Optics Scanning Light Ophthalmoscopy. Transl Vis Sci Technol 6:9

Showing the most recent 10 out of 486 publications