Imaging/Histopathology Core. This core has had excellent productivity for the past five years of support. Numerous investigators have used the core, increased their collaborative efforts, and have greatly increased their own productivity. Because a major strength of this Core grant is publications, a list of 170 of 215 total for years 2008-2012 years of funding for this Core grant is provided (See Appendix, Publications) to concretely demonstrate the activity, and collaborative efforts for vision research made possible by this facility at WSU. Eighteen investigators (12 with 15 NEI. ROl grants) utilized the facility. Seven utilized the l/H core extensively, nine moderately and 2 had limited usage. Table 2 lists past investigators, and their degree of usage. Annual detailed reports submitted to the NEI fully document the past progress and collaborative efforts enhanced by this core also and are available for each year of P30 funding. Therefore, the following section only briefly highlights some of the studies that have been facilitated by this core either by use of its equipment and/or research personnel. The l/H core is integral to NEI funded studies and has also, albeit at lower priority, assisted more junior vision investigators with pilot studies (e.g., Drs. Huang, Singh, Kumar, Thummel and Tkatchenko). Specifically, the core has assisted in studies on: comparative histological analyses and ultra-high resolution MRI of retina; histopathological studies of new orthotopic models of choroidal melanoma;in situ hybridization against the segmentation gene wingless;paraffin embedment and sectioning of retinas and corneas for immunolabeling; slit lamp of normal and infected mouse and rat eyes;the immunochemical and histological characterization of transgenic mouse retinas by light and electron microscopy;studies of human type-2 diabetes in a rat model; histopathological evaluation of retinal ischemia models;histopathology of recombinant protein and antibody neutralized mice;characterization of cytokine knockout vs. wild-type mice in P. aeruginosa keratitis, with focus on growth factors, their receptors and modulation of Toll-like receptors;confocal microscopic dual immunolabeling for VIP and macrophage expression of its receptor;transmission EM of RNAi knockdown in grasshopper eyes for analysis of gene function during postembryonic retinal development;bacterial flagellin studies in P. aeruginosa keratitis;training of research personnel in cryosectioning, confocal laser scanning microscopy and use of the Apotome and Leica microscopes as well as poster printing and assistance with graphics for publications. For all of these studies and many others not included here, this core and its research assistants, Ronald Barrett and Norbert Wolf, provided excellent technical expertise. Concrete evidence of this support from 2008- 2012, is reflected by the list of 170 representative papers shown of a total of 215. In this regard, 142 of the 215 publications had assistance from this facility. In addition, another 33 papers (of 215 total) had assistance from both the l/H and Tc/Mol core, described below. (See Appendix, Publications).

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
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Study Section
Special Emphasis Panel (ZEY1-VSN (03))
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Wayne State University
United States
Zip Code
Curtiss, Elizabeth; Jiang, Youde; Liu, Li et al. (2018) Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines. Mediators Inflamm 2018:3809092
Singh, Pawan Kumar; Khatri, Indu; Jha, Alokkumar et al. (2018) Determination of system level alterations in host transcriptome due to Zika virus (ZIKV) Infection in retinal pigment epithelium. Sci Rep 8:11209
Han, Jing; Li, Yue; Liu, Xiuli et al. (2018) Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo. PLoS One 13:e0193031
Berkowitz, Bruce A; Podolsky, Robert H; Berri, Ali M et al. (2018) Dark Rearing Does Not Prevent Rod Oxidative Stress In Vivo in Pde6brd10 Mice. Invest Ophthalmol Vis Sci 59:1659-1665
Jiang, Youde; Liu, Li; Steinle, Jena J (2018) miRNA15a regulates insulin signal transduction in the retinal vasculature. Cell Signal 44:28-32
Liu, Li; Patel, Paragi; Steinle, Jena J (2018) PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose. Inflamm Res 67:1013-1019
Carion, Thomas W; Greenwood, Matthew; Ebrahim, Abdul Shukkur et al. (2018) Immunoregulatory role of 15-lipoxygenase in the pathogenesis of bacterial keratitis. FASEB J 32:5026-5038
Shi, Haoshen; Berger, Elizabeth A (2018) Characterization of Site-Specific Phosphorylation of NF-?B p65 in Retinal Cells in Response to High Glucose and Cytokine Polarization. Mediators Inflamm 2018:3020675
Guest, John M; Singh, Pawan Kumar; Revankar, Sanjay G et al. (2018) Isavuconazole for Treatment of Experimental Fungal Endophthalmitis Caused by Aspergillus fumigatus. Antimicrob Agents Chemother 62:
Berkowitz, Bruce A; Podolsky, Robert H; Qian, Haohua et al. (2018) Mitochondrial Respiration in Outer Retina Contributes to Light-Evoked Increase in Hydration In Vivo. Invest Ophthalmol Vis Sci 59:5957-5964

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