of the core: The Tc/Mol core, located in dedicated space in the Department of Anatomy/Cell Biology, provides a centralized facility for maintenance and availability of immortalized cell lines and assistance with tissue and organ culture. In addition, the core will continue to assist investigators in the use of various techniques in molecular biology. [New to the core is the addition of virus production capability, which will include assistance on HEK cell line culture for AAV vector packaging and purification, spearheaded by Dr. Pan, the core director]. An experienced research assistant, Kerry Vistisen, will be available to assist investigators and their staff who have little expertise in these areas to develop experimental methods relevant to their studies. In addition, she will assist investigators with performance of molecular and other (Western blot) techniques. Projects proposed requiring core use will be presented to the core director (Dr. Pan) for consultation. Once approved, the projects will be prioritized, with NEI R01 funded projects given priority and undertaken by the core research assistant. This may involve performance of the task, or training of personnel-or both. If two projects require similar personnel training, for example, the research assistant will consolidate training to be most efficient whenever possible. Space and equipment for molecular studies have also expanded and these changes have enhanced the use of this core. In fact, R01 funded investigators who lack experience in the technology required for molecular biology, tissue/organ culture, and/or virus production will still rely heavily on the equipment and availability of the research assistant for technical advice or hands on assistance for their projects. Those knowledgeable in the technical aspects involved will use the facility to enhance their own studies and to begin pilot or collaborative research projects. This centralized core will thus continue to provide a reasonable, cost efficient mechanism to facilitate and enhance numerous NEI R0l funded research projects as its top priority. It is also the goal of the core to enhance, at lower priority, collaboration and productivity of those who are performing pilot studies in vision, by providing some financial offset (See letter of support. Dr. Stanton and Administrative core) which will encourage interaction with scientists from diverse fields, with those in vision and potentially attract them to vision research.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY004068-31A1
Application #
8877736
Study Section
Special Emphasis Panel (ZEY1-VSN (03))
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
31
Fiscal Year
2014
Total Cost
$107,831
Indirect Cost
$37,083
Name
Wayne State University
Department
Type
DUNS #
001962224
City
Detroit
State
MI
Country
United States
Zip Code
48202
Li, Cui; McClellan, Sharon A; Barrett, Ronald et al. (2014) Interleukin 17 regulates Mer tyrosine kinase-positive cells in Pseudomonas aeruginosa keratitis. Invest Ophthalmol Vis Sci 55:6886-900
Hazlett, Linda D; Jiang, Xiaoyu; McClellan, Sharon A (2014) IL-10 function, regulation, and in bacterial keratitis. J Ocul Pharmacol Ther 30:373-80
Jiang, Xiaoyu; McClellan, Sharon A; Barrett, Ronald et al. (2014) HGF signaling impacts severity of Pseudomonas aeruginosa keratitis. Invest Ophthalmol Vis Sci 55:2180-90
Ivanova, Elena; Lee, Patrick; Pan, Zhuo-Hua (2013) Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line. J Comp Neurol 521:2165-80
Lu, Qi; Ivanova, Elena; Ganjawala, Tushar H et al. (2013) Cre-mediated recombination efficiency and transgene expression patterns of three retinal bipolar cell-expressing Cre transgenic mouse lines. Mol Vis 19:1310-20
Deng, Qiuchan; Sun, Mingxia; Yang, Kun et al. (2013) MRP8/14 enhances corneal susceptibility to Pseudomonas aeruginosa Infection by amplifying inflammatory responses. Invest Ophthalmol Vis Sci 54:1227-34
Foldenauer, Megan E B; McClellan, Sharon A; Berger, Elizabeth A et al. (2013) Mammalian target of rapamycin regulates IL-10 and resistance to Pseudomonas aeruginosa corneal infection. J Immunol 190:5649-58
Devi, Takhellambam S; Hosoya, Ken-Ichi; Terasaki, Tetsuya et al. (2013) Critical role of TXNIP in oxidative stress, DNA damage and retinal pericyte apoptosis under high glucose: implications for diabetic retinopathy. Exp Cell Res 319:1001-12
Thomas, Jennifer L; Thummel, Ryan (2013) A novel light damage paradigm for use in retinal regeneration studies in adult zebrafish. J Vis Exp :e51017
Singh, Lalit P (2013) Thioredoxin Interacting Protein (TXNIP) and Pathogenesis of Diabetic Retinopathy. J Clin Exp Ophthalmol 4:

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